Original Article

Serological study of Human Fasciolosis in Patients Referring to the School of Public Health, Tehran University of Medical Sciences, Tehran, Iran during 2008-2014


Background: Fascioliasis is a zoonotic disease of livestock and human caused by Fasciola species. Here in, the results of serological evaluation of fascioliasis in peo­ple referring to the School of Public Health, Tehran University of Medical Sci­ences during 2008-2014 are presented.

Methods: Demographic characterizations, symptoms and eosinophil rate were registered for every patient. Using somatic antigen of Fasciola, ELISA was per­formed and the results were analyzed. Data of questioners were analyzed as well.

Results: Among 206 applicants, 24.8% were seropositive for fascioliasis, included 21% female and 28.3% male. Mean range of age of patients was between 13 to 67 yr. The highest rate of seropositivity was found among 20-30 yr old patients. Most of the patients had hypereosinophilia. All patients had history of eating raw vegeta­bles, or drinking unsafe water. Patients were referring from different provinces of Iran, including Gilan, Mazandaran, Tehran, Ardabil, Khuzestan, Lorestan, North Khorasan, Kermanshah, Azerbaijan, Fars, Kordestan, Hamedan and Markazi.

Conclusion: During recent years, variety of provinces in Iran, where patients with fascioliasis are referred, has been increased. Patients coming from Gilan and Mazanda­ran provinces were referred early after the onset of their symptoms. Most probably, physicians in Gilan and Mazandaran are more alert on fascioliasis than other provinces. Previous wrong diagnosis was more common among patients refer­ring from other provinces than Gilan and Mazandaran provinces.

Rokni MB, Lotfy WM, Ashrafi K,Murrell KD (2014). Fasciolosis in the MENA Region. In: Neglected Tropical Diseases - Middle East and North Africa. Ed(s), McDowell MA, Rafati S: Springer Vienna.

Rokni M (2008). The present status of human helminthic diseases in Iran. Ann Trop Med Parasitol, 102:283-295.

Mas-Coma S, Bargues MD,Valero MA (2005). Fascioliasis and other plant-borne trematode zoonoses. Int J Parasitol, 35:1255-1278.

Rokni MB, Massoud J. O'Neill SM, Parkinson M, Dalton JP. Diagnosis of human fasciolosis in the Gilan province of Northern Iran: application of cathepsin L-ELISA. Diag Microbiol Infect Dis. 2002;44:175.

Dalton JP. Fasciolosis. 1st ed. CABI Publishing Wallingford, UK, Oxon. 1999.

Rokni MB, Aminian B. Evaluation of the Enzyme-linked Immuno-electro Transfer Blot (EITB) technique using hydatid cyst antigens B/5 and total IgG antibodies in laboratory diagnosis of human hydatidosis. Pak J Med Sci. 2006;22:127-131.

Mas-Coma S, Bargues M. Human liver flukes: a review. Res Rev Parasitol. 1997; 57:145-218.

Rokni M, Lesan S, Massoud J, Kia E, Gh M. Comparative evaluation of Fast enzyme linked immunosorbent assay (Fast-ELISA) and standard-ELISA for the diagnosis of human hydatidosis. Iran J Public Health. 2006; 35(2):29-32.

Rokni M, Massoud J, Hanilo A. Comparison of adult somatic and cysteine proteinase antigens of Fasciola gigantica in enzyme linked immunosorbent assay for serodiagnosis of human fasciolosis. Acta Trop. 2003;88:69-75.

Awad WS, Ibrahim AK, Salib FA. Using indirect ELISA to assess different antigens for the serodiagnosis of Fasciola gigantica infection in cattle, sheep and donkeys. Res Vet Sci. 2009;86:466-471.

Hillyer GV. Immunodiagnosis of human and animal fasciolosis. In: Fasciolosis. Ed(s), Dalton JP. Oxon: CABI Publishing, Wallin-gford, UK, 1998; p. 435-48.

Massoud J. Fasciolosis outbreak in man and drug test (Triclabandazole) in Caspian littoral, northern part of Iran. Bull Soc France Parasitol. 1989; 8:438.

Assmar M, Milaninia A, Amir-Khani A, Yadegari D, Forghan-Parast K, Nahravanian H, Piazak N, Esmayli A, Hovanesian A,Valadk-hani Z. Seroepidemiological investigation of fascioliasis in northern Iran. Medical Journal of the Islamic Republic of Iran. 1991;5:23-27.

Forghan-Parast K,Ashrafi K. Comparison of the formalin-ether and Kato-Katz in the parasitological diagnosis of human fascioliasis. J Med Fac Gilan Univ Med Sci Iran. 2001; 9:1-6.

Ashrafi K, Saadat F, Rahmati B. Evaluating the level of endemicity of human fascioliasis in rural communities of Bandar-e-Anzali district; an important step for implementation of disease control programs. First International and 8th National Congress of Parasitology and Parasitic Diseases in Iran, Kerman, Iran. 2012; p. 121.

WHO. (2010) The" Neglected" Neglected Worms. Action Against Worms. December 2007, issue 10.

Sarkari B, Ghobakhloo N, Moshfea A, Eilami O. Seroprevalence of human fasciolosis in a new-emerging focus of fasciolosis in yasuj district, southwest of iran. Iran J Parasitol. 2012; 7:15-20.

Hatami H, Asmar M, Masoud J, Mansouri F, Namdaritabar H, Ramazankhani A. The First Epidemic and New-emerging Human Fascioliasis in Kermanshah (Western Iran) and a Ten-year Follow Up, 1998-2008. Int J Prev Med. 2012; 3:266-72.

Rokni MB. Fasciola hepatica and F. gigantica. In: Encyclopedia of Food Safety. Ed(s), Motarjemi Y: ELSEVIER Publication. 2014.

IssueVol 10 No 4 (2015) QRcode
SectionOriginal Article(s)
Fascioliasis ELISA Raw Vegetable Iran

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
How to Cite
ARYAEIPOUR M, KIA EB, HEIDARI Z, SAYYAD TALAIE Z, ROKNI MB. Serological study of Human Fasciolosis in Patients Referring to the School of Public Health, Tehran University of Medical Sciences, Tehran, Iran during 2008-2014. Iran J Parasitol. 2015;10(4):517-522.