Articles

Cloning and Sequencing of Leishmania major Thiol-Specific-Antioxidant Antigen (TSA) Gene

Abstract

Background: Leishmaniasis is caused by parasitic protozoa of the genus Leishmania which, in the infected host are obli­gate intracellular parasite. TSA is the immuno-dominant antigen of Leishmania major which is considered as the most promising molecule for a recombinant or DNA vaccine against leishmaniasis.

Methods: Genomic DNA of TSA protein was extracted and amplificated as a template. Then the PCR product was cloned into pTZ57R/T vector. Finally, the recombinant plasmid was extracted from transformed Escherichia coli (TG1 strain) and sequenced.

Results: MRHO/IR/75/ER (an Iranian strain) of L. major and TSA gene (Accession number LmjF15.1080) were used. Se­quence analysis of cloned TSA gene into pTZ57R/T vector showed high homology of 90% with LmjF15.1080 (TSA gene) and strain "LV39" (Accession no. AF069386) and strain "Friedlin" (Accession no.AF044679).

Conclusion: We cloned TSA gene of L. major successfully. Recombinant plasmid was confirmed. It is ready to express recombinant protein for further studies.

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IssueVol 2 No 4 (2007) QRcode
SectionArticles
Keywords
Sequencing TSA
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Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
How to Cite
1.
F Tabatabaie, F Ghaffari far, A Dalimi, Z Sharifi, A Zavaran Hoseini. Cloning and Sequencing of Leishmania major Thiol-Specific-Antioxidant Antigen (TSA) Gene. Iran J Parasitol. 1;2(4):30-41.