Tehran University of Medical Sciences Iranian Journal of Parasitology 1735-7020 2 4 2007 12 15 30 41 F Tabatabaie F Ghaffari far A Dalimi Z Sharifi A Zavaran Hoseini 2015 10 03 Background: Leishmaniasis is caused by parasitic protozoa of the genus Leishmania which, in the infected host are obli­gate intracellular parasite. TSA is the immuno-dominant antigen of Leishmania major which is considered as the most promising molecule for a recombinant or DNA vaccine against leishmaniasis. Methods: Genomic DNA of TSA protein was extracted and amplificated as a template. Then the PCR product was cloned into pTZ57R/T vector. Finally, the recombinant plasmid was extracted from transformed Escherichia coli (TG1 strain) and sequenced. Results: MRHO/IR/75/ER (an Iranian strain) of L. major and TSA gene (Accession number LmjF15.1080) were used. Se­quence analysis of cloned TSA gene into pTZ57R/T vector showed high homology of 90% with LmjF15.1080 (TSA gene) and strain "LV39" (Accession no. AF069386) and strain "Friedlin" (Accession no.AF044679). Conclusion: We cloned TSA gene of L. major successfully. Recombinant plasmid was confirmed. It is ready to express recombinant protein for further studies. https://ijpa.tums.ac.ir/index.php/ijpa/article/view/40 https://ijpa.tums.ac.ir/index.php/ijpa/article/download/40/39