Molecular and Morphological Data Confirmed First Record of Abbreviata kazakhstanica Markov and Paraskiv, 1956 (Spirurida: Physalopteridea) in Iran
-
Morteza Ghanbari Johkool
,
Aida Vafae Eslahi
,
Milad Badri
,
Elham Hooshmand
,
Majid Pirestani
,
Daniel Jablonski
,
Rasool Jafari
,
Abdolhossein Dalimi
Abstract
Background: The genus Abbreviata (Spirurida: Physalopteridea) currently contains 47 species. Physalopteridae nematodes infect a large number of vertebrates, including mammals, birds, reptiles and amphibians. The current study is a report of the first morphological and molecular identification of A. kazakhstanica (Spirurida: Physalopteridea) in Pseudopus apodus in Iran.
Methods: Eleven road-killed P. apodus, were collected from, Iran during 2016-2018. The nematodes were isolated from stomach. After morphological study, the genomic DNA of the parasites was extracted using CTAB method. The DNA was used for PCR amplification of cytochrome c oxidase subunit I (cox1). The PCR products were sequenced, the sequence data were analyzed and multiple alignments were conducted using the Clustal Omega.
Results: After detailed microscopic examination, the A. kazakhstanica was identified. The cox1 sequences confirmed the species of helminth. The new sequences of A. kazakhstanica were submitted to GenBank under the accession number MK578751-2.
Conclusion: Regarding the limited data on parasitological status of Iranian reptiles, more specific and comprehensive investigations are needed to identify the parasitic fauna.
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19. Yap K, Thompson R. CTAB precipitation of cestode DNA. Parasitol Today. 1987; 3(7):220-2.
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21. Gafurov A, Lunkina E. A study of the developmental cycle of Abbreviata kazachstanica Markov & Paraskiv, 1956 (Nematoda: Spirurata). Izvestiya Akademii Nauk Tadzhikskoi SSR (Ahboroti Akademijai Fanhoi RSS Tociston) Otdelenie Biologicheskikh Nauk. 1970; 4(41)):100-104.
22. Kabilov T, Siddikov BK, editors. Identification of intermediate hosts of nematode Abbreviata kazakhstanica Markov et Paraskiv 1956. Dokl Akad Nauk Uzb SSR; 1978.
23. Vakker V, Brushko Z, Kolbintsev V. Parasite fauna of Ophisaurus apodus in the Kazakh SSR, USSR Izv. An Kazakhskoi SSR Biol. 1985; 4:36-9.
24. Markov G, Paraskiv K. The helminth fauna of reptiles in Kazakhstan. Trudy Instituta Zoologii Akademiya Nauk Kazakhskoi SSR. 1956; 5:120-8.
25. Kabilov T. [Life cycle of the nematode, Abbreviata kazachstanica]. Parazitologiia. 1980; 14(3):263-70.
2. Rastegar-Pouyani N, Kami HG, Rajabzadeh M, et al. Annotated checklist of amphibians and reptiles of Iran. Iranian Journal of Animal Biosystematics (Ijab) . 2008; 4(1): 7–30.
3. Çiçek K, Tok CV, Hayretdağ S, et al. Data on the food composition of European Glass Lizard, Pseudopus apodus (Pallas, 1775)(Squamata: Anguidae) from Çanakkale (Western Anatolia, Turkey). Acta Zool Bulg. 2014; 66(3):433-6.
4. Šmid J, Moravec J, Kodym P, et al. Annotated checklist and distribution of the lizards of Iran. Zootaxa. 2014; 3855:1-97.
5. Chabaud AG. Un nouveau physaloptere parasite d’agame. Ann Parasitol Hum Comp1953; 28(4):305-11.
6. Rezazadeh E, Tajbakhs F, Bursey CR, et al. Helminth parasites of the Caucasian agama, Laudakia caucasia (Squamata: Agamidae), from Iran. Comp. Parasitol. 2012; 79(1):160-163.
7. Hosseinzadeh AA, Lagzian A. First report of Oswaldo filaria chlamydosauri, Breinl, 1912 (Nematoda: Onchocercidae) from a new host Paralaudakia caucasia, Eichwald, 1831 (Squamata: Agamidae) and its prevalence and intensity in Mashhad, North-eastern Iran. JWB. 2018; 2(1):6-11.
8. Halajian A, Bursey CR, Goldberg SR, et al. Helminth parasites of the European glass lizard, Pseudopus apodus (Squamata: Anguidae), and European grass snake, Natrix natrix (Serpentes: Colubridae), from Iran. Comparative Parasitology. 2013; 80(1):151-156.
9. Rahimian H, Pazoki S, Habashi SA. Gastrointestinal nematodes of Laudakia nupta nupta (Sauria: Agamidae) from Iran with descriptions of two new species (Oxyuridea: Pharyngodonidae) and comments on the diagnostic features of Parapharyngodon and Thelandros. Zootaxa. 2014; 3852(1):51-82.
10. Pazoki S, Rahimian H. New species of Spauligodon Skrjabin, Schikhobalova & Lagodovskaja, 1960 and Thubunea Seurat, 1914 (Nematoda) from the gastro-intestinal tract of lizards in Iran. Syst Parasitol. 2014; 89(3):259-70.
11. Dollfus R-P. Mission Yves-J. Golvan et Jean-A. Rioux en Iran-Cestodes de Carnivores, Rongeurs, Insectivores, Reptiles et Batraciens. Ann Parasitol Hum Comp. 1965; 40(1):61-86.
12. Goldberg S, Bursey C. Ophisops elegans (snake-eyed lizard): endoparasites. Herpetol Rev. 2010; 41:495.
13. Anderson RC. Nematode parasites of vertebrates: their development and transmission. CABI Publishing, Wallingford UK. 2000.
14. King C, Tay CY, Jones HIJJowd. Morphologic Observations and Novel 18S rRNA Sequences of Abbreviata hastaspicula and Abbreviata antarctica from Varanus spp. Lizards in Australia. J Wildl Dis. 2019; 55(1):149-152.
15. Jablonski D, Vági B, Kardos G. Abbreviata abbreviata (Rudolphi, 1819) as a new nematode parasite for Malpolon insignitus (Geoffroy Saint-Hilaire, 1827) recorded in Albania. Ecologica Montenegrina. 2015;2(3):194-196.
16. Morgan BB. Host-parasite relationships and, geographical distribution of the Physalopterinae (Nematoda). Trans Wis Acad Sci Arts Lett. 1947; 38:273-92.
17. King C, Jones H, Tay CY. Arthropod intermediate hosts of Abbreviata antarctica (Nematoda: Physalopteridae) in Australia. J Parasitol. 2013; 99(4):708-11.
18. Jones HI. Physalopterine nematodes in Australian reptiles: interactions and patterns of infection. Aust J Zool. 2014; 62(2):180-194.
19. Yap K, Thompson R. CTAB precipitation of cestode DNA. Parasitol Today. 1987; 3(7):220-2.
20. Bowles J, Blair D, McManus DP. Genetic variants within the genus Echinococcus identified by mitochondrial DNA sequencing. Mol Biochem Parasitol. 1992;54(2):165-73.
21. Gafurov A, Lunkina E. A study of the developmental cycle of Abbreviata kazachstanica Markov & Paraskiv, 1956 (Nematoda: Spirurata). Izvestiya Akademii Nauk Tadzhikskoi SSR (Ahboroti Akademijai Fanhoi RSS Tociston) Otdelenie Biologicheskikh Nauk. 1970; 4(41)):100-104.
22. Kabilov T, Siddikov BK, editors. Identification of intermediate hosts of nematode Abbreviata kazakhstanica Markov et Paraskiv 1956. Dokl Akad Nauk Uzb SSR; 1978.
23. Vakker V, Brushko Z, Kolbintsev V. Parasite fauna of Ophisaurus apodus in the Kazakh SSR, USSR Izv. An Kazakhskoi SSR Biol. 1985; 4:36-9.
24. Markov G, Paraskiv K. The helminth fauna of reptiles in Kazakhstan. Trudy Instituta Zoologii Akademiya Nauk Kazakhskoi SSR. 1956; 5:120-8.
25. Kabilov T. [Life cycle of the nematode, Abbreviata kazachstanica]. Parazitologiia. 1980; 14(3):263-70.
| Files | ||
| Issue | Vol 16 No 4 (2021) | |
| Section | Short Communication(s) | |
| DOI | https://doi.org/10.18502/ijpa.v16i4.7882 | |
| Keywords | ||
| Abbreviata kazakhstanica Molecular identification Pseudopus apodus Physalopteridea Iran | ||
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How to Cite
1.
Johkool M, Vafae Eslahi A, Badri M, Hooshmand E, Pirestani M, Jablonski D, Jafari R, Dalimi A. Molecular and Morphological Data Confirmed First Record of Abbreviata kazakhstanica Markov and Paraskiv, 1956 (Spirurida: Physalopteridea) in Iran. Iran J Parasitol. 2021;16(4):686-691.
