Tehran University of Medical Sciences Iranian Journal of Parasitology 1735-7020 16 4 2021 11 24 686 691 Morteza Johkool Metabolic Diseases Research Center, Research Institute for Prevention of Non-Communicable Diseases, Qazvin University of Medical Sciences, Qazvin, Iran Aida Vafae Eslahi Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Milad Badri Metabolic Diseases Research Center, Research Institute for Prevention of Non-Communicable Diseases, Qazvin University of Medical Sciences, Qazvin, Iran Elham Hooshmand Department of Parasitology, Faculty of Veterinary Medicine, Rasht Branch, Islamic Azad University, Guilan Majid Pirestani Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Daniel Jablonski Department of Zoology, Comenius University in Bratislava, Mlynská dolina, Ilkovičova 6, 842 15 Bratislava, Slovakia Rasool Jafari Department of Medical Parasitology and Mycology, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran Abdolhossein Dalimi Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran 2020 04 22 2021 05 17 Background: The genus Abbreviata (Spirurida: Physalopteridea) currently contains 47 species. Physalopteridae nematodes infect a large number of vertebrates, including mammals, birds, reptiles and amphibians. The current study is a report of the first morphological and molecular identification of A. kazakhstanica (Spirurida: Physalopteridea) in Pseudopus apodus in Iran. Methods: Eleven road-killed P. apodus, were collected from, Iran during 2016-2018. The nematodes were isolated from stomach. After morphological study, the genomic DNA of the parasites was extracted using CTAB method. The DNA was used for PCR amplification of cytochrome c oxidase subunit I (cox1). The PCR products were sequenced, the sequence data were analyzed and multiple alignments were conducted using the Clustal Omega. Results: After detailed microscopic examination, the A. kazakhstanica was identified. The cox1 sequences confirmed the species of helminth. The new sequences of A. kazakhstanica were submitted to GenBank under the accession number MK578751-2. Conclusion: Regarding the limited data on parasitological status of Iranian reptiles, more specific and comprehensive investigations are needed to identify the parasitic fauna. https://ijpa.tums.ac.ir/index.php/ijpa/article/view/2895 https://ijpa.tums.ac.ir/index.php/ijpa/article/download/2895/1184