Original Article

Plasmodium berghei ANKA Infection in ICR Mice as a Model of Cerebral Malaria


Background: Animal models with various combination of host-parasite have long been employed to study malaria pathogenesis. Here, we describe the combination of Plasmodium berghei ANKA infec- tion in inbred ICR mice as a model of cerebral malaria (CM).

Methods: Infection in mice was initiated by intraperitoneal injection of 2 x 107  (0.2ml) parasitized red blood cells (PRBCs).

Results: This model can produce a severe degree of infection presented by the high degree of parasitaemia followed by death 6-7 days post infection. Severe anemia, splenomegaly, hepatomegaly and discolourations of major organs were observed. Histopathological findings revealed several i m- portant features mimicking human CM including, microvascular sequestration of PRBCs in major organs, particularly in the brain, hypertrophy and hyperplasia of the kupffer cells in the liver, pulmo- nary edema and hyaline membrane formation in the lungs and haemorrhages in the kidney’s medulla and cortex. Proinflammatory cytokines TNFα, IFNγ, IL-1, IL-6 and IL-18, and anti-inflammatory cytokine IL-10 were all found to be elevated in the plasma of infected mice.

Conclusion: This model can reproduce many of the important features of CM and therefore can be used as a tool to advance our understanding of the disease pathogenesis.

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IssueVol 7 No 4 (2012) QRcode
SectionOriginal Article(s)
Animal model Malaria Plasmodium berghei ICR mice

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How to Cite
Basir R, Fazalul-Rahiman S, Hasballah K, Chong W, Talib H, Yam M, Jabbarzare M, Tie T, Othman F, Moklas M, Abdullah W, Ahmad Z. Plasmodium berghei ANKA Infection in ICR Mice as a Model of Cerebral Malaria. Iran J Parasitol. 2015;7(4):62-74.