Tehran University of Medical Sciences Iranian Journal of Parasitology 1735-7020 7 4 2015 10 13 62 74 EN R. Basir Department of Human Anatomy, Faculty of Medicine & Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia. SS. Fazalul-Rahiman Department of Human Anatomy, Faculty of Medicine & Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia. K. Hasballah Department of Pharmacology, Faculty of Medicine, Syiah Kuala University, 23111 Darussalam, Banda Aceh, Nanggroe Aceh Darussalam, Indonesia. WC. Chong Department of Human Anatomy, Faculty of Medicine & Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia. H. Talib Department of Pathology, Faculty of Medicine & Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia. MF. Yam Pharmacology Discipline, School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia. M. Jabbarzare Department of Human Anatomy, Faculty of Medicine & Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia. TH. Tie Department of Human Anatomy, Faculty of Medicine & Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia. F. Othman Department of Human Anatomy, Faculty of Medicine & Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia. MAM. Moklas Department of Human Anatomy, Faculty of Medicine & Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia. WO. Abdullah Department of Medical Microbiology and Parasitology, Faculty of Medicine & Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia. Z. Ahmad Department of Pathology, Faculty of Medicine & Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia. 2015 10 13 2015 10 13 Background: Animal models with various combination of host-parasite have long been employed to study malaria pathogenesis. Here, we describe the combination of Plasmodium berghei ANKA infec- tion in inbred ICR mice as a model of cerebral malaria (CM). Methods: Infection in mice was initiated by intraperitoneal injection of 2 x 107  (0.2ml) parasitized red blood cells (PRBCs). Results: This model can produce a severe degree of infection presented by the high degree of parasitaemia followed by death 6-7 days post infection. Severe anemia, splenomegaly, hepatomegaly and discolourations of major organs were observed. Histopathological findings revealed several i m- portant features mimicking human CM including, microvascular sequestration of PRBCs in major organs, particularly in the brain, hypertrophy and hyperplasia of the kupffer cells in the liver, pulmo- nary edema and hyaline membrane formation in the lungs and haemorrhages in the kidney’s medulla and cortex. Proinflammatory cytokines TNFα, IFNγ, IL-1, IL-6 and IL-18, and anti-inflammatory cytokine IL-10 were all found to be elevated in the plasma of infected mice. Conclusion: This model can reproduce many of the important features of CM and therefore can be used as a tool to advance our understanding of the disease pathogenesis. https://ijpa.tums.ac.ir/index.php/ijpa/article/view/285 https://ijpa.tums.ac.ir/index.php/ijpa/article/download/285/268