Original Article

Prevalence and Genotyping of Trichomonas vaginalis Infected to dsRNA Virus by PCR–Restriction Fragment Length Polymorphism (RFLP)


Background: Trichomonas vaginalis is a prevalent sexually transmitted infection cause trichomoniasis. In this study prevalence and genotype of Iranian isolates of T. vaginalis infected (dsRNA) viruses were evaluated by PCR-RFLP and obtained patterns were then confirmed by sequence analysis and genotype of these Iranian isolates confirmed again.

Methods: Ten strains of T.vaginalis were collected from 1700 vaginal samples of women referred to hospitals associated with Iran University of Medical Sciences in Tehran, Iran during Feb 2016 to Jul 2017, evaluated in points of infection to T. vaginalis Virus (TVV-1) were used in a PCR-RFLP. All of ten isolates of T. vaginalis were examined by designed nested PCR for actin gene and then digestion patterns of three endonuclease enzymes of HindII, MseI and RsaI were evaluated and genotype of these isolates was defined.

Results: By combination of fragments pattern of three enzymes of HindII, RsaI and MseI, three genotypes were found; six genotypes E, two genotypes G and two genotypes I. The most dominant genotypes were genotype E. Among four TVV infected isolates two genotype E, one genotype G and one genotype I were found, however among six uninfected T. vaginalis isolates to TVV-1, all of three genotypes were also found.

Conclusion: Three genotypes E, G and I in T. vaginalis infected with dsRNA isolates were found, however, these three genotypes in T. vaginalis without virus were also observed. Further study is needed to evaluate genotypes of T. vaginalis, which infected virus in more great T. vaginalis population.

Petrin D, Delgaty K, Bhatt R, Garber G. Clinical and microbiological aspects ofTrichomonas vaginalis. Clinical microbiology reviews. 1998;11(2):300-17.

Kharsany A, Hoosen A, Moodley J, Bagaratee J, Gouws E. The association between sexually transmitted pathogens and cervical intra-epithelial neoplasia in a developing community. Sex Transm Infect. 1993;69(5):357-60.

Heine P, McGREGOR JA. Trichomonas vaginalis: a reemerging pathogen. Clin Obstet Gynecol. 1993;36(1):137-44.

Grodstein F, Goldman MB, Cramer DW. Relation of tubal infertility to history of sexually transmitted diseases. Am J Epidemiol. 1993;137(5):577-84.

Laga M, Nzila N, Goeman J. The interrelationship of sexually transmitted diseases and HIV infection: implications for the control of both epidemics in Africa. AIDS. 1991;5:S55-63.

Crucitti T, Abdellati S, Van Dyck E, Buvé A. Molecular typing of the actin gene of Trichomonas vaginalis isolates by PCR–restriction fragment length polymorphism. Clinical Microbiology and Infection. 2008;14(9):844-52.

Singh B. Molecular methods for diagnosis and epidemiological studies of parasitic infections. International Journal for Parasitology. 1997;27(10):1135-45.

Matini M, Rezaie S, Mohebali M, Maghsood A-H, Rabiee S, Fallah M, et al. Genetic identification of Trichomonas vaginalis by using the actin gene and molecular based methods. Iranian journal of parasitology. 2014;9(3):329.

VAN̂ÁĈOVÁ Ŝ, Tachezy J, KULDA J, FLEGR J. Characterization of trichomonad species and strains by PCR fingerprinting. Journal of Eukaryotic Microbiology. 1997;44(6):545-52.

Fraga J, Rodriguez J, Fuentes O, Fernandez-Calienes A, Castex M. Optimization of random amplified polymorphic DNA techniques for use in genetic studies of Cuban Triatominae. Rev Inst Med Trop Sao Paulo. 2005;47(5):295-300.

Snipes LJ, Gamard PM, Narcisi EM, Beard CB, Lehmann T, Secor WE. Molecular epidemiology of metronidazole resistance in a population of Trichomonas vaginalis clinical isolates. J Clin Microbiol. 2000;38(8):3004-9.

Momeni Z, Sadraei J, Kazemi B, Dalimi A. Molecular typing of the actin gene of Trichomonas vaginalis isolates by PCR-RFLP in iran. Experimental parasitology. 2015;159:259-63.

Alderete J, Wendel K, Rompalo A, Erbelding E, Benchimol M, Chang T-H. Trichomonas vaginalis: evaluating capsid proteins of dsRNA viruses and the dsRNA virus within patients attending a sexually transmitted disease clinic. Exp Parasitol. 2003;103(1):44-50.

Fraga J, Rojas L, Sariego I, Fernández-Calienes A. Double-stranded RNA viral infection in Cuban Trichomonas vaginalis isolates. Brazilian Journal of Infectious Diseases. 2005;9(6):521-4.

Khoshnan A, Alderete J. Trichomonas vaginalis with a double-stranded RNA virus has upregulated levels of phenotypically variable immunogen mRNA. J Virol. 1994;68(6):4035-8.

Bessarab IN, Liu H-W, Ip C-F, Tai J-H. The complete cDNA sequence of a type II Trichomonas vaginalis virus. Virology. 2000;267(2):350-9.

Sommer U, Costello CE, Hayes GR, Beach DH, Gilbert RO, Lucas JJ, et al. Identification of Trichomonas vaginalis cysteine proteases that induce apoptosis in human vaginal epithelial cells. J Biol Chem. 2005;280(25):23853-60.

Bessarab IN, Nakajima R, Liu H-W, Tai J-H. Identification and characterization of a type III Trichomonas vaginalis virus in the protozoan pathogen Trichomonas vaginalis. Arch Virol. 2011;156(2):285-94.

Weber B, Mapeka T, Maahlo M, Hoosen A. Double stranded RNA virus in South African Trichomonas vaginalis isolates. J Clin Pathol. 2003;56(7):542.

El-Gayar EK, Mokhtar AB, Hassan WA. Molecular characterization of double-stranded RNA virus in Trichomonas vaginalis Egyptian isolates and its association with pathogenicity. Parasitol Res. 2016:1-10.

Wendel KA, Rompalo AM, Erbelding EJ, Chang T-H, Alderete JF. Double-stranded RNA viral infection of Trichomonas vaginalis infecting patients attending a sexually transmitted diseases clinic. J Infect Dis. 2002;186(4):558-61.

Khanaliha K, Masoumi-Asl H, Bokharaei-Salim F, Tabatabaei A, Naghdalipoor M. Double-stranded RNA viral infection of Trichomonas vaginalis (TVV1) in Iranian isolates. Microb Pathog. 2017.

Pillay A, Liu H, Chen CY, Holloway B, Sturm WA, Steiner B, et al. Molecular Subtyping ofTreponema pallidumSubspeciespallidum. Sex Transm Dis. 1998;25(8):408-14.

Goodman RP, Ghabrial SA, Fichorova RN, Nibert ML. Trichomonasvirus: a new genus of protozoan viruses in the family Totiviridae. Arch Virol. 2011;156(1):171-9.

Fraga J, Rojas L, Sariego I, Fernández-Calienes A. Genetic characterization of three Cuban Trichomonas vaginalis virus. Phylogeny of Totiviridae family. Infect Genet Evol. 2012;12(1):113-20.

Bricheux G, Brugerolle G. Molecular cloning of actin genes in Trichomonas vaginalis and phylogeny inferred from actin sequences. FEMS microbiology letters. 1997;153(1):205-13.

Matini M, Rezaei H, Fallah M, Maghsood AH, Saidijam M, Shamsi-Ehsan T. Genotyping, Drug Susceptibility and Prevalence Survey of Trichomonas vaginalis among Women Attending Gynecology Clinics in Hamadan, Western Iran, in 2014-2015. Iranian Journal of Parasitology. 2017;12(1):29-37.

OLIAEE RT, BABAEI Z, HATAM GR, KARESHK AT, MAHMOUDVAND H, VAFAFAR A, et al. Considerable Genetic Diversity of Trichomonas vaginalis Clinical Isolates in a Targeted Population in South of Iran. Iranian Journal of Parasitology. 2017;12(2):251-9.

Hampl V, Vaňáčová Š, Kulda J, Flegr J. Concordance between genetic relatedness and phenotypic similarities of Trichomonas vaginalis strains. BMC evolutionary biology. 2001;1(1):11.

IssueVol 14 No 2 (2019) QRcode
SectionOriginal Article(s)
DOI https://doi.org/10.18502/ijpa.v14i2.1137
Genotype Actin RFLP Trichomonas vaginalis

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How to Cite
ORUJZADEH F, TABATABAIE F, KHANALIHA K, AKHLAGHI L, BOKHARAEI-SALIM F, FALLAH S, ESTEGHAMATI A, MASOUMI-ASL H. Prevalence and Genotyping of Trichomonas vaginalis Infected to dsRNA Virus by PCR–Restriction Fragment Length Polymorphism (RFLP). Iran J Parasitol. 2019;14(2):250-257.