Case Report

Trypanosoma evansi and “Candidatus Mycoplasma haemolamae” Co-Infection in One-Humped Camel (Camelus dromedarius) from the Northwest of Iran: A Case Report


A 4-year-old male one-humped camel (Camelus dromedarius) was referred to Veterinary Teaching Hospital of Urmia University, Iran in 2017 with anorexia, weakness, depression and pale mucosa. Decreased red blood cell count, packed cell volume and hemoglobin concentration were detected by complete blood cell count. In Giemsa-stained peripheral blood smears Trypanosoma spp. trypomastigotes scattered between erythrocytic spaces and Mycoplasma-like organisms were observed attached to the surface of erythrocytes. Species-specific PCR assay confirmed T. evansi and Candidatus Mycoplasma haemolamae (CMhl) co-infection. Administration of diminazene aceturate, oxytetracycline 20%, flunixin meglumine and phosphorus-vitamin B12 were not effective in treatment. Hemoplasmosis should be considered as an important differential diagnosis of conditions associated with hemolytic anemia in camel.

Pacholek X, Gamatie D, Franck SV, Tibayrenc R. Prevalence of Trypanosomaevansitrypanosomiasis in young camels in West Niger. Revue d'élevageet de médecinevétérinaire des pays tropicaux. 2000;53(2):177-82.

Ahmadi-hamedani M, Ghazvinian K, Darvishi MM. Hematological and serum biochemical aspects associated with a camel (Camelusdromedarius) naturally infected by Trypanosomaevansi with severe parasitemia in Semnan, Iran. Asian Pacific Journal of Tropical Biomedicine. 2014;4(9):743-5.

Sow A, Sidibé I, Kalandi M, Bathily A, Ndiaye N, Ouédraogo M, et al. Biochemical changes induced by natural infection of trypanosomosis in Burkinabese local donkey breeds. Comparative Clinical Pathology. 2014;23(1):103-9.

Zarif-Fard M, Hashemi-Fesharki R. Study on tissue and blood protozoa of camels in southern Iran. Journal of Camel Practice and Research. 2000;7(2):193-4.

Reagan W, Garry F, Thrall M, Colgan S, Hutchison J, Weiser M. The clinicopathologic, light, and scanning electron microscopic features of eperythrozoonosis in four naturally infected llamas. Veterinary pathology. 1990;27(6):426-31.

McLaughlin BG, Evans CN, McLaughlin PS, Johnson L, Smith A, Zachary J. An Eperythrozoon-like parasite in llamas. Journal of the American Veterinary Medical Association. 1990;197(9):1170-5.

Crosse P, Ayling R, Whitehead C, Szladovits B, English K, Bradley D, et al. First detection of ‘Candidatus Mycoplasma haemolamae’infection in alpacas in England. Veterinary Record Case Reports. 2013;1(1):e100611.

Khosravi A, Parizi MH, Bamorovat M, Zarandi MB, Mohammadi MA. Prevalence of Trypanosomaevansi in camels using molecular and parasitological methods in the southeast of Iran, 2011. Journal of parasitic diseases. 2015;39(3):422-5.

Mirshekar F, Yakhchali M, Shariati-Sharifi F. Trypanosomaevansi infection and major risk factors for Iranian one-humped camels (Camelusdromedarius). Journal of Parasitic Diseases.1-5.

Farooq U, Samad H, Khurshid A, Sajjad S. Normal reference Haematolo-gical values of one-humped camels (CamelusDromedarius) kept in Cholistan desert. J Anim Plant Sci. 2011;21(2):157-60.

Tornquist SJ, Boeder LJ, Cebra CK, Messick J. Use of a polymerase chain reaction assay to study response to oxytetracycline treatment in experimental Candidatus Mycoplasma haemolamae infection in alpacas. American journal of veterinary research. 2009;70(9):1102-7.

Ashour AA, El-Naga TRA, Barghash SM, Salama MS. Trypanosomaevansi: Detection of Trypanosomaevansi DNA in naturally and experimentally infected animals using TBR 1 & TBR 2 primers. Experimental parasitology. 2013;134(1):109-14.

Moghaddar N, Diantpour V. Distribution pattern of Trypanosomaevansi in camels (Camelusdromedarius) in Iran. Journal of Camel Practice and Research. 2009;16(1):73-5.

Sazmand A, Rasooli A, Nouri M, Hamidinejat H, Hekmatimoghaddam S. Serobiochemical alterations in subclinically affected dromedary camels with Trypanosomaevansi in Iran. Pak Vet J. 2011;31(3):223-6.

Logan-Henfrey LL, Gardiner P, Mahmoud MM. Animal trypanosomiases in sub-Saharan Africa. 1992.2:157–276.

Tornquist SJ, Boeder LJ, Lubbers S, et al. Investigation of Mycoplasma haemolamae infection in crias born to infected dams. The Veterinary Record 2011;168:380a.

Nazifi S, Oryan A, Bahrami S, Razavi S. Evaluation of haematological and serum biochemical parameters in Iranian camels (Camelusdromedarius) infected with haemotrophic Mycoplasma (Eperythrozoon) spp. Comparative Clinical Pathology. 2009;18(3):329-32.

Saleh MA, Al-Salahy MB, Sanousi SA. Oxidative stress in blood of camels (Camelus dromedaries) naturally infected with Trypanosoma evansi. Veterinary Parasitology. 2009;162(3):192-9.

IssueVol 14 No 2 (2019) QRcode
SectionCase Report(s)
Dromedary camel Trypanosoma Mycoplasma PCR Iran

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ESMAEILNEJAD B, SAADI A, DALIR-NAGHADEH B, SAMIEI A, MOHAMMADI V, PIRNEJAD-TALATAPEH A, EHTESHAMFAR S. Trypanosoma evansi and “Candidatus Mycoplasma haemolamae” Co-Infection in One-Humped Camel (Camelus dromedarius) from the Northwest of Iran: A Case Report. Iran J Parasitol. 2019;14(2):347-351.