Efficacy of Pistacia khinjuk Fruits on Viability of Hydatid Cyst Protoscoleces and Its Acute Toxicity in Mice Model
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Hossein MAHMOUDVAND
,
Seyed Reza MIRBADIE
,
Mehdi GHASEMI KIA
,
Ebrahim BADPARVA
,
Saeedeh SHAMSADINI LORI
,
Majid FASIHI HARANDI
Abstract
Background: This investigation aimed to evaluate the in vitro scolicidal effects of Pistacia khinjuk methanolic extract against protoscoleces of hydatid cysts and its acute toxicity in mice NMRI model.
Methods: Protoscoleces were aseptically extracted from sheep livers having hydatid cysts. Various concentrations of the essential oil (12.5- 100 mg/mL) were used for 10 to 60 min. Viability of protoscoleces was confirmed using eosin exclusion test (0.1% eosin staining). Twenty-four male NMRI mice were used to assess the acute toxicity of P. khinjuk.
Results: P. khinjuk extract at the concentrations of 100 mg/mL after 10 min of exposure killed 100% of protoscoleces. Similarly, the mean of mortality rate of protoscoleces after 20 min of exposure to the concentration of 50 mg/mL was 100%. The LD50 of the intraperitoneal injection of the P. khinjuk methanolic extract was 2.8 g/kg and the maximum non-fatal dose was 1.7 g/kg.
Conclusion: The findings demonstrated effective scolicidal effects of P. khinjuk extract with no considerable toxicity that might be a natural source for the producing of new scolicidal agent.World Health Organization (WHO) informal working group on echinococcosis. Bull WHO 1996; 74: 231–42
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| Files | ||
| Issue | Vol 11 No 3 (2016) | |
| Section | Short Communication(s) | |
| Keywords | ||
| Scolicidal Cystic echinococcosis Hydatid cyst Echinococcus granulosus Toxicity | ||
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