Tehran University of Medical Sciences Iranian Journal of Parasitology 1735-7020 11 3 2016 12 21 383 388 Hossein MAHMOUDVAND Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran Seyed Reza MIRBADIE School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran Mehdi GHASEMI KIA Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University, Kerman, Iran Ebrahim BADPARVA Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran Saeedeh SHAMSADINI LORI Research Center for Hydatid Disease in Iran, Kerman University of Medical Sciences, Kerman, Iran Majid FASIHI HARANDI Research Center for Hydatid Disease in Iran, Kerman University of Medical Sciences, Kerman, Iran 2016 09 28 2016 09 28 Background: This investigation aimed to evaluate the in vitro scolicidal effects of Pistacia khinjuk methanolic extract against protoscoleces of hydatid cysts and its acute toxicity in mice NMRI model. Methods: Protoscoleces were aseptically extracted from sheep livers having hydatid cysts. Various concentrations of the essential oil (12.5- 100 mg/mL) were used for 10 to 60 min. Viability of protoscoleces was confirmed using eosin exclusion test (0.1% eosin staining). Twenty-four male NMRI mice were used to assess the acute toxicity of P. khinjuk. Results: P. khinjuk extract at the concentrations of 100 mg/mL after 10 min of exposure killed 100% of protoscoleces. Similarly, the mean of mortality rate of protoscoleces after 20 min of exposure to the concentration of 50 mg/mL was 100%. The LD50 of the intraperitoneal injection of the P. khinjuk methanolic extract was 2.8 g/kg and the maximum non-fatal dose was 1.7 g/kg. Conclusion: The findings demonstrated effective scolicidal effects of P. khinjuk extract with no considerable toxicity that might be a natural source for the producing of new scolicidal agent. https://ijpa.tums.ac.ir/index.php/ijpa/article/view/1210 https://ijpa.tums.ac.ir/index.php/ijpa/article/download/1210/719