Effect of zinc on humoral and cell-mediated immunity of broilers vaccinated against coccidiosis.

  • Sobhan Sajadifar Department of Epidemiology and Parasitology, Faculty of Veterinary Medicine and Zootechnics, Armenian National Agrarian University, Yerevan, Armenia.
  • Hadi Miranzadeh Department of Veterinary, University of Applied Science and Technology, Institute of Science Applied Higher Education of Jahad -e- Agriculture, Isfahan, Iran.
  • Milad Moazeni Department of Veterinary, University of Applied Science and Technology, Institute of Science Applied Higher Education of Jahad -e- Agriculture, Isfahan, Iran.
Keywords: Coccidiosis, Immunity, Zinc


 Background: The aim of the present study was the comparison of humoral and cell-mediated immunity in broilers fed with different levels of zinc during a coccidiosis challenge. Methods: One hundred and forty- four one-day-old broiler chicks were used with three dietary zinc (40, 120 and 200 mg/kg). At 14 d of age, all birds were inoculated orally with 5×103 sporulated oocysts of E. Tenella. At 2, 22, 32, 42 days of age, the blood serums were tested for antibody titer against New-castle disease vaccine, using the standard HI test. On day 42 the sum of ni-trite and nitrate based on the reduction of nitrate to nitrite by cadmium and white blood cell count (WBC) using a hemocytometer were measured. Results: At 42 d, levels of 120 and 200 mg significantly (P< 0.05) increased the antibody titer in compare with the control. The peak response of CBH was observed at the level of 200 mg Zn/kg diet. Also both level of 120 and 200 mg Zn/kg diet increased WBC count and sum of nitrite and nitrate in serum compared with the control. Conclusion: The levels of 120 and 200 mg Zn/kg diet could be considered as a non-pharmacologic booster of immunity in broilers chicks infected with E. Tenella.


Williams RB. Epidemiological studies of coccidiosis in the domestic foal (Gallus gallus). Apple Parasitol. 1995; 36: 90-96.

Fekete SG, Kellems RO. Interrelationship of feeding with immunity and parasitic infection: a review. Veterinarni Medicina. 2007; 52: 131–143.

Shankar AH, Prasad AS. Zinc and immune function: the biological basis of altered re-sistance to infection. Am J Clin Nutr. 1998; 68: 447S–463S.

Baker DH, Ammerman CB. Zinc Bioavailabil-ity. Academic Press, New York. 1995; 367–398.

Cardoso A, Albuquerque R, Tessari E. Humoral immunological response in broilers vaccinated against Newcastle disease and sup-plemented with dietary zinc and vitamin E. Avian Andrade Neves. 2007; 2501–2509.

Bartlett JR, Smith MO. Effects of different levels of zinc on the performance and immunocompetence of broilers under heat stress. Poultry Science. 2003; 82: 1580–1588.

National Research Council. Nutrition Re-quirements of Poultry. 9th Rev, Edition Na-tional Academy Press, Washington, DC. 1994.

Lillehoj HS, Ruff MD. Comparison of disease susceptibility and sub-class antibody response in SC and FP chickens experimentally inoculat-ed with Eimeria tenella or Eimeria maxima. Avian Dis. 1987; 31: 112–119.

Allan WH, Gough RE. A standard Hemagglutination Inhibition test for Newcastle disease. A comparison of macro and micro methods. Vet Rec. 1974; 95: 120–123.

Navarro-Gonzalvez JA, Garcia-Benayas C, Arenas J. Semiautomated measurement of ni-trate in biological fluids. Am Assoc Clin Chem. 1998; 679–681.

SAS Institute: SAS User's Guide. Statistics. SAS Institute, Inc., Cary, NC. 1997.

Kidd MT, Qureshi MA, Ferket PR, Thomas LN. Turkey hen zinc source affects progeny immunity and disease resistance. J Appl Poult Res. 2000; 9: 414–423.

Fraker PJ, Gershwin ME, Good RA, Prasad AS. Interrelationships between zinc and im-mune function. Fed Proc. 1986; 45: 1474-1479.

Nassiri-Moghadam H, Jahanian R. Immuno-logical responses of broiler chicks can be mod-ulated by dietary supplementation of zinc-methionine in place of inorganic zinc sources. Asian-Aust J Anim Sci. 2009; 22(3): 396–403.

Sunder GS, Panda AK, Gopinath NCS, Rama Rao SV, Raju MVLN, Reddy MR, Vijay Ku-mar C. Effects of higher levels of zinc supple-mentation on performance, mineral availability, and immune competence in broiler chickens. J Appl Poult Res. 2008; 17: 79–86.

Kidd MT, Ferket PR, Qureshi MA. Zinc me-tabolism with special reference to its role in immunity. World Poult Sci J. 1996; 52: 309–323.

Maggini S, Wintergerst ES, Beveridge S, Hornig DH. Selected vitamins and trace ele-ments support immune function by strength-ening epithelial barriers and cellular and humoral immune responses. Br J Nutr. 2007; 98(1): S29–S35.

Al-Daraji HJ, Amen MHM. Effect of dietary Zinc on certain blood traits of broiler breeder chickens. Int J Poult Sci. 2011; 10 (10): 807–813.

Hosseini S, Arshami J, Torshizi E. Viral anti-body titer and leukocyte subset responses to graded copper and zinc in broiler chicks. Asian J Anim & Vet Adv. 2011; 6(1): 80–87.

Tanaka Y, Shiozawa S, Morimoto I, Fujita T. Role of zinc in interleukin 2 (IL 2) mediated T-cell activation. Scand J Immunol. 1990; 31: 547–552.

James SJ, Swendseid M, Makinodan T. Macro-phage-mediated depression of T- cell prolifera-tion in zinc-deficient mice. J Nutr. 1987; 117: 1982–1988.

Sunzel B, Holm S, Reuterving CO, Soderberg T, Hallmans G, Hanstrom L. The effect of zinc on bacterial phagocytosis, killing and cytoprotection in human polymorphonuclear leucocytes. APMIS. 1995; 103: 635–644.

Tizard IR. Veterinary Immunology: An Intro-duction. 8th ed. Saunders Elsevier, St. Louis, MO. 2009.

How to Cite
Sajadifar S, Miranzadeh H, Moazeni M. Effect of zinc on humoral and cell-mediated immunity of broilers vaccinated against coccidiosis. Iran J Parasitol. 8(3):474-480.