Iranian Journal of Parasitology 2013. 8(4):570-578.

Prevalence and Molecular Diagnosis of Babesia ovis and Theileria ovis in Lohi Sheep at Livestock Experiment Station (LES), Bahadurnagar, Okara, Pakistan.
Waseem Shahzad, Haider Noor, Mansur-Ud-Din Ahmad, Rashid Munir, Muhammad Sharif Saghar, Muhammad Hassan Mushtaq, Nisar Ahmad, Ghulam Akbar, Fayyaz Mehmood



Background: Babesia ovis and Theileria ovis are among the important and main etiological agents causing ovine babesiosis and ovine theileriosis, causing severe economic losses among sheep and goats. The aim of the present study was to determine the prevalence and molecular diagnosis of B. ovis and T. ovis in Lohi sheep at Livestock Experiment Station Bahadurnagar, Okara, Pakistan.

Methods: The prevalence of B. ovis and T. ovis was investigated in 200 Lohi sheep of mixed age and sex by PCR during 2011. The assay was employed us-ing primers Bbo-F & Bbo-R, specific for a 549-bp fragment in B. ovis genomic DNA and primers TSsr 170F & TSsr 670R, specific for a 520-bp fragment in T. ovis genomic DNA. The animals were also screened for both haemoparasites through stained thin blood smears.

Results: Thirty two (16%), 48 (24%) and 26 (13%) were the number of ani-mals found positive for B. ovis, T. ovis and for mixed infection with both para-sites respectively, through microscopy. Sixty eight (34%), 73 (37%) and 42 (21%) were the number of animals found positive for B. ovis, T. ovis and for mixed infection with both parasites respectively, through PCR test.

Conclusion: The results indicate the high sensitivity of PCR for surveying babesiosis and theileriosis and there is noteworthy prevalence of these diseases in sheep at an experimental station where environmental conditions are rela-tively controlled as compared to field conditions.


Babesia ovis; PCR; Pakistan; Sheep; Theileria ovis

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