Canine Babesiosis in China Caused by Babesia gibsoni: A Molecular Approach.
AbstractBackground: To provide a point of reference to study the epidemiology and clin-ical expression of canine babesiosis in China.Methods: A total of 30 dogs infected with canine babesiosis were evaluated by mean of clinical history, physical examination, hematological, restriction fragment length polymorphism of PCR products (PCR-RFLP) and sequencing analysis.Result: The most prevalent clinical abnormalities were lethargy (100%), anorexia (100%), pale or icteric mucous membranes (80%), fever (70%) and dark urine (70%). Hematology parameters revealed that anemia and thrombocytopenia were the major abnormalities in blood of dogs infected with canine babesia. The results of PCR-RFLP and sequencing analysis indicated that B. gibsoni was the main species responsible for canine babesiosis cases at the time of the study in Nanjing, China.Conclusions: The results provide valuable information for better understanding of the epidemiology of canine babesiosis in China.
Zygner W, Gojska O, Rapacka G, Jaros D, Wedrychowicz H. Hematological changes during the course of canine babesiosis caused by large Babesia in domestic dogs in Warsaw (Poland). Vet Parasitol. 2007; 145: 146-151.
Boozer AL, Macintire DK. Canine babesiosis. Vet Clin N Am-Small. 2003; 33: 885-904.
Costa-Junior LM, Zahler-Rinder M, Ribeiro MFB, et al. Use of a Real Time PCR for detecting subspecies of Babesia canis. Vet Parasitol. 2012; 188: 160-163.
Irwin P. Canine babesiosis: from molecular taxonomy to control. Parasite and Vector. 2009; 2(Suppl 1): S4.
Rene-Martellet M, Chene J, Chabanne L, Chalvet-Monfray K, Bourdoiseau G. Clinical signs, seasonal occurrence and causative agents of canine babesiosis in France: Results of a multiregional study. Vet Parasitol. 2013; 197: 50-58.
Birkenheuer AJ, Levy MG, Breitschwerdt EB. Development and evaluation of a seminested PCR for detection and differentiation of Babesia gibsoni (Asian genotype) and B-canis DNA in canine blood samples. J Clin Microbiol. 2003; 41: 4172-4177.
Caccio SM, Antunovic B, Moretti A, et al. Molecular characterisation of Babesia canis canis and Babesia canis vogeli from naturally infected European dogs. Vet Parasitol. 2002; 106: 285-292.
Li GQ, Zhang HJ, Zhang GL, et al. Molecular identification and phylogenetic analysis of Babe-sia vogeli from dogs. Chinese Veterinary Science. 2011; 41: 245-249.
Sadeghi Dehkordi Z, Zakeri S, Nabian S, et al. Molecular and biomorphometrical identifica-tion of ovine babesiosis in Iran. Iranian J Para- sitol. 2010; 5(4): 21-30.
Yamane I, Thomford JW, Gardner IA, et al. Evaluation of the indirect fluorescent antibody test for diagnosis of Babesia gibsoni infections in dogs. Am J Vet Res. 1993; 54: 1579-1584.
Martin AR, Dunstan RH, Roberts TK, et al. Babesia canis vogeli: a novel PCR for its detection in dogs in Australia. Exp Parasitol. 2006; 112: 63-65.
Fabisiak M, Sapierzynski R, Klucinski W. Analysis of haematological abnormalities observed in dogs infected by a large Babesia. B Vet I Pulawy. 2010; 54: 167-170.
Jefferies R, Ryan UM, Irwin PJ. PCR-RFLP for the detection and differentiation of the canine piroplasm species and its use with filter paper-based technologies. Vet Parasitol. 2007; 144: 20-27.
Ikadai H, Tanaka H, Shibahara N, et al. Molecular evidence of infections with Babesia gibsoni parasites in Japan and evaluation of the diagnostic potential of a loop-mediated isothermal amplification method. J Clin Microbiol. 2004; 42: 2465-2469.
Gulanber A, Gorenflot A, Schetters TPM, Carcy B. First molecular diagnosis of Babesia vogeli in domestic dogs from Turkey. Vet Parasitol. 2006; 139: 224-230.
Inokuma H, Yoshizaki Y, Matsumoto K, et al. Molecular survey of Babesia infection in dogs in Okinawa, Japan. Vet Parasitol. 2004; 121: 341-46