Tehran University of Medical Sciences Iranian Journal of Parasitology 1735-7020 9 2 2014 06 15 163 168 EN Da-Wei Yao College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu, China. Jing-Ya Jiang College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu, China. Ze-Zhong Yu YuXi Agriculture Vocation-Technical College, Yuxi, Yunnan, China. Dong-Qin Yao College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu, China. De-Ji Yang College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu, China. Yan-Bing ZhAO College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu, China. 2015 10 14 Background: To provide a point of reference to study the epidemiology and clin-ical expression of canine babesiosis in China. Methods: A total of 30 dogs infected with canine babesiosis were evaluated by mean of clinical history, physical examination, hematological, restriction fragment length polymorphism of PCR products (PCR-RFLP) and sequencing analysis. Result: The most prevalent clinical abnormalities were lethargy (100%), anorexia (100%), pale or icteric mucous membranes (80%), fever (70%) and dark urine (70%). Hematology parameters revealed that anemia and thrombocytopenia were the major abnormalities in blood of dogs infected with canine babesia. The results of PCR-RFLP and sequencing analysis indicated that B. gibsoni was the main species responsible for canine babesiosis cases at the time of the study in Nanjing, China. Conclusions: The results provide valuable information for better understanding of the epidemiology of canine babesiosis in China. https://ijpa.tums.ac.ir/index.php/ijpa/article/view/417 https://ijpa.tums.ac.ir/index.php/ijpa/article/download/417/327