Absence of Asymptomatic Malaria Infection in Endemic Area of Bashagard District, Hormozgan Province, Iran
AbstractBackground: A successful malaria elimination program calls for enough attention to parasite carriers, especially asymptomatic malaria, as well as the diagnosis and treatment of clinical cases. Asymptomatic malaria is an infection that patients do not show any symptom; thus, these patients play critical role in the concept of an elimination program. The current investigation was conducted to evaluate the presence of these cases in Bashagard District, formerly a high malaria transmission area in Hormozgan Province, Iran.Methods: Blood samples (n=500) were collected from symptomless individuals residing in Bashagard to evaluate Plasmodium infection by using microscopic, serological and nested-PCR techniques.Results: Regarding the microscopic and nested-PCR analysis, no asymptomatic infection was detected among studied individuals. Totally, 1% of the studied population (5 of 500) had anti PvMSP-119-specific IgG antibody; however, only 0.2% (1 of 500) of the individuals was seropositive to recombinant PfMSP-119, using ELISA.Conclusion: This study showed no asymptomatic malaria infection in the studied population; hence malaria elimination is feasible and can be successfully carried out in this region.
Suárez-Mutis MC, Cuervo P, Leoratti FM, Moraes-Avila SL, Ferreira AW, Fernandes O, Coura JR. Cross sectional study reveals a high percentage of asymptomatic Plasmodium vivax infection in the Amazon Rio Negro area, Brazil. Rev Inst Med Trop Sao Paulo. 2007; 49(3):159-64.
Harris I, Sharrock WW, Bain LM, Gray KA, Bobogare A, Boaz L, Lilley K, Krause D, Vallely A, Johnson ML, Gatton ML, Shanks GD, Cheng Q. A large proportion of asymptomatic Plasmodium infections with low and sub-microscopic parasite densities in the low transmission setting of Temotu Province, Solomon Islands: challenges for malaria diagnostics in an elimination setting. Malar J. 2010; 9:254.
Bousema JT, Gouagna LC, Drakeley CJ, Meutstege AM, Okech BA, Akim IN, Beier JC, Githure JI, Sauerwein RW. Plasmodium falciparum gametocyte carriage in asymptomatic children in western Kenya. Malar J. 2004; 3:18.
Alves FP, Durlacher RR, Menezes MJ, Krieger H, Silva LH, Camargo EP. High prevalence of asymptomatic Plasmodium vivax and Plasmodium falciparum infections in native Amazonian populations. Am J Trop Med Hyg. 2002; 66(6):641-8.
Eke R A, Chigbu L N, Nwachukwu w. High Prevalence of Asymptomatic Plasmodium Infection in a Suburb of Aba Town, Nigeria. Ann Afr Med. 2006; 5(1):42-5.
Males S, Gaye O, Garcia A. Long-term asymptomatic carriage of Plasmodium falciparum protects from malaria attacks: a prospective study among Senegalese children. Clin Infect Dis. 2008; 46(4):516-22.
Nkoghe D, Akue JP, Gonzalez JP, Leroy EM. Prevalence of Plasmodium falciparum infection in asymptomatic rural Gabonese populations. Malar J. 2011; 10(1):33.
Bin Mohanna MA, Bin Ghouth AS, Rajaa YA. Malaria signs and infection rate among asymptomatic schoolchildren in Hajr Valley, Yemen. East Mediterr Health J. 2007; 13(1):35-40.
Coleman RE, Maneechai N, Rachaphaew N, Kumpitak C, Miller RS, Soyseng V, Thimasarn K, Sattabongkot J. Comparison of field and expert laboratory microscopy for active surveillance for asymptomatic Plasmodium falciparum and Plasmodium vivax in western Thailand. Am J Trop Med Hyg. 2002; 67(2):141-4.
Richards AK, Smith L, Mullany LC, Lee CI, Whichard E, Banek K, Mahn M, ShweOo EK, Lee TJ. Prevalence of Plasmodium falciparum in active conflict areas of eastern Burma: a summary of cross-sectional data. Confl Health. 2007; 1:9.
Coura JR, Suárez-Mutis M, Ladeia-Andrade S. A new challenge for malaria control in Brazil: asymptomatic Plasmodium infection--a review. Mem Inst Oswaldo Cruz. 2006; 101(3):229-37.
Roshanravan B, Kari E, Gilman RH, Cabrera L, Lee E, Metcalfe J, Calderon M, Lescano AG, Montenegro SH, Calampa C, Vinetz JM. Endemic malaria in the Peruvian Amazon region of Iquitos. Am J Trop Med Hyg. 2003; 69(1):45-52.
John CC, Riedesel MA, Magak NG, Lindblade KA, Menge DM, Hodges JS, Vulule JM, Akhwale W. Possible interruption of malaria transmission, highland Kenya, 2007-2008.Emerg Infect Dis. 2009; 15(12):1917-24.
Fernando SD, Abeyasinghe RR, Galappaththy GN, Rajapaksa LC. Absence of asymptomatic malaria infections in previously high endemic areas of Seri Lanka. Am J Trop Med Hyg. 2009; 81(5):763-7.
Bottius E, Guanzirolli A, Trape JF, Rogier C, Konate L, Druilhe P. Malaria: even more chronic in nature than previouslythought: evidence for subpatent parasitaemia detectable by the polymerase chainreaction. Trans R Soc Trop Med Hyg 1996; 90:15–9.
Schneider P, Bousema JT, Gouagna LC, Otieno S, van de Vegte-Bolmer M, Omar SA, Sauerwein RW. Submicroscopic Plasmodium falciparum gametocyte densities frequently result in mosquito infection. Am J Trop Med Hyg 2007; 76:470-74.
Coleman RE, Kumpitak C, Ponlawat A, Maneechai N, Phunkitchar V, Rachapaew N, Zollner G, Sattabongkot J. Infectivity of asymptomatic Plasmodium-infected human populations to Anopheles dirus mosquitoes in western Thailand. J Med Entomol 2004, 41:201-208.
Roper C, Elhassan IM, Hviid L, Giha H, Richardson W, Babiker H, Satti GM, Theander TG, Arnot DE. Detection of very low level Plasmodium falciparum infections using the nested polymerase chain reaction and a reassessment of the epidemiology of unstable malaria in Sudan. Am J Trop Med Hyg 1996, 54:325-331.
Nateghpour M, Turki H, Keshavarz H, Edrissian G H, Mohebali M, Forushani A R. A Parasitological and serological study in Malaria suspected patient in Hormozgan Province, Southeastern Iran. Iran Red Crescent Med J. 2010; 12(3):242-246.
Mehrizi AA, Zakeri S, Salmanian AH, Sanati MH, Djadid ND. IgG subclasses pattern and high-avidity antibody to the C terminal region of merozoite surface protein 1 of Plasmodium vivax in an unstable hypoendemic region in Iran. Acta Trop. 2009; 112(1):1-7.
Zakeri S, Mehrizi AA, Zoghi S, DjadidND. Non-variant specific antibody responses to the C-terminal region of merozoite surface protein-1 of Plasmodium falciparum (PfMSP-1(19)) in Iranians exposed to unstable malaria transmission. Malar J. 2010; 9:257.
Snounou G, Viriyakosol S, Jarra W, Thaithong S, Brown KN. Identification of the four human malaria parasite species in field samples by the polymerase chain reaction and detection of a high prevalence of mixed infections. Mol Biochem Parasitol. 1993; 58:283-92.
Coleman RE, Sattabongkot J, Promstaporm S, Maneechai N, Tippayachai B, Kengluecha A, Rachapaew N, Zollner G, Miller RS, Vaughan JA, Thimasarn K, Khuntirat B. Comparison of PCR and microscopy for the detection of asymptomatic malaria in a Plasmodium falciparum/vivax endemic area in Thailand. Malar J. 2006; 5: 121.
Dawoud HA, Ageely HM, Heiba AA. Comparison of two commercial assays and microscopy with PCR for diagnosis of malaria. J Egypt Soc Parasitol. 2008; 38(2):329-38.
Nateghpour M, Akbarzadeh K, Farivar L, Amiri A. Detection of asymptomatic malaria infection among the Afghani immigrant population in Iranshahr district of southeastern Iran. Bull Soc Pathol Exot. 2011;104(4):321-3.