Efficacy of Artemisinin Base Combination Therapy and Genet-ic Diversity of Plasmodium falciparum from Uncomplicated Malaria Falciparum Patient in District of Pesawaran, Province of Lampung, Indonesia

  • Jhons Fatriyadi SUWANDI Department of Microbiology and Parasitology, Faculty of Medicine, University of Lampung, Bandar Lampung, Indonesia
  • Widya ASMARA Department of Microbiology, Faculty of Veterinary Medicine, Gadjah Mada University, Jogjakarta, Indonesia
  • Hari KUSNANTO Department of Public Health, Faculty of Medicine, Public Health and Nursing, Gadjah Mada University, Jogjakar-ta, Indonesia
  • Din SYAFRUDDIN Department of Parasitology, Faculty of Medicine, Hasanuddin University, Makasar, Indonesia Eijkman Institute for Molecular Biology, Jakarta, Indonesia
  • Supargiyono SUPARGIYONO Department of Parasitology, Faculty of Medicine, Public Health and Nursing, Gadjah Mada University, Jogjakarta, Indonesia
Keywords: Artemisinin combination therapy, Efficacy, Genetic variation, Plasmodium falciparum

Abstract

Background: Malaria is an infectious disease caused by Plasmodium sp., that still prevalence in some part of Indonesia. District of Pesawaran is one of malaria endemic area in the Province of Lampung. The purpose of this study was to evaluate the efficacy of the ACT treatment in the District of Pesawaran Province of Lampung, Indonesia from Dec 2012 to Jul 2013 and the genetic variation of the Plasmodium falciparum also studied. Methods: This study was observational analytic study of falciparum malaria patients treated with ACT and primaquine (DHP-PQ and AAQ-PQ) at Hanura Primary Health Centre (Puskesmas). DNA isolation was done with QIAmp DNA Mini Kit. Amplification of PfMDR1, MSP1, and MSP2 genes was done with appropriate forward and reverse primer and procedures optimized first. PCR Product of PfMDR1 gene was prepared for sequencing. Data analysis was done with MEGA 6 software. Results: The results of this research are DHP-PQ effectiveness was still wellness among falciparum malaria patients in District of Pesawaran, Province of Lampung, Indonesia. There is Single-nucleotide mutation of N86Y of PfMDR1 gene. The dominant alleles found are MAD20 and 3D7 alleles with Multiplicity of Infection (MOI) are low. Conclusion: Therapy of DHP-PQ as an antimalarial falciparum in Pesawaran District, Lampung, Indonesia is still good. The genetic variation found was the SNP on the N86Y PfMDR1 gene, with dominant allele MAD20 and 3D7.

References

Ministry of Health of the Republic of Indonesia. Indonesia Health Profile Year 2010. Jakarta: Ministry of Health Republic of Indonesia; 2011.

Ministry of Health of the Republic of Indonesia. Guidelines for Managing Malaria Cases in Indonesia. Jakarta: Directorate General of Disease Control and Environmental Health; 2011.

Ministry of Health of the Republic of Indonesia. Handbook of Malaria Case Management. Jakarta: Ministry of Health Republic of Indonesia; 2012.

Pesawaran District Health Office. Annual Parasite Incidence Year 2010 and 2011. Pesawaran, Lampung; 2012.

Ministry of Health of the Republic of Indonesia. Guidelines for Managing Malaria Cases in Indonesia. Jakarta: Ministry of Health Republic of Indonesia; 2008.

Reed MB, Saliba KJ, Caruana SR, Kirk K, Cowman a F. Pgh1 modulates sensitivity and resistance to multiple antimalarials in Plasmodium falciparum. Nature [Internet]. 2000 Mar 24;403(6772):906–9. Available from: http://www.ncbi.nlm.nih.gov/pubmed/10706290

Warhurst DC. A Molecular Marker For Chloquinine Resistant Falciparum Malaria. N Engl J Med. 2001;344(4):299–302.

Dokomajilar C, Lankoande ZM, Dorsey G, Zongo I, Ouedraogo J-B, Rosenthal PJ. Roles of specific Plasmodium falciparum mutations in resistance to amodiaquine and sulfadoxine-pyrimethamine in Burkina Faso. Am J Trop Med Hyg [Internet]. 2006 Jul;75(1):162–5. Available from: http://www.ncbi.nlm.nih.gov/pubmed/16837725

Dokomajilar C, Nsobya SL, Greenhouse B, Rosenthal PJ, Dorsey G. Selection of Plasmodium falciparum pfmdr1 alleles following therapy with artemether-lumefantrine in an area of Uganda where malaria is highly endemic. Antimicrob Agents Chemother [Internet]. 2006 [cited 2014 May 22];50(5):1893–5. Available from: http://aac.asm.org/content/50/5/1893.short

Humphreys GS, Merinopoulos I, Ahmed J, Whitty CJM, Mutabingwa TK, Sutherland CJ, et al. Amodiaquine and Artemether-Lumefantrine Select Distinct Alleles of the Plasmodium falciparum mdr1 Gene in Tanzanian Children Treated for Uncomplicated Malaria ᰔ. Antimicrob Agents Chemother. 2007;51(3):991–7.

Dahlström S. Role of PfATP6 and pf MRP1 in Plasmodium falciparum resistance to antimalarial drugs. Stockholm: Karolinska Institutet; 2009.

Ferreira PE, Holmgren G, Veiga MI, Uhlén P, Kaneko A, Gil JP. PfMDR1: mechanisms of transport modulation by functional polymorphisms. PLoS One [Internet]. 2011 Jan [cited 2014 Apr 22];6(9):e23875. Available from: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=3164660&tool=pmcentrez&rendertype=abstract

WHO. Assessment and Monitoring of Antimalarial Drug Efficacy for the Treatment of Uncomplicated Falciparum Malaria. Geneva: WHO, The Institute; 2003.

Dahlström S, Veiga MI, Ferreira P, Mårtensson A, Kaneko A, Andersson B, et al. Diversity of the sarco/endoplasmic reticulum Ca(2+)-ATPase orthologue of Plasmodium falciparum (PfATP6). Infect Genet Evol [Internet]. 2008 May [cited 2014 Mar 7];8(3):340–5. Available from: http://www.ncbi.nlm.nih.gov/pubmed/18359278

Thanh NX, Trung TN, Phong NC, Thien NX, Dai B, Shanks GD, et al. Open label randomized comparison of dihydroartemisinin-piperaquine and artesunate-amodiaquine for the treatment of uncomplicated Plasmodium falciparum malaria in central Vietnam. [Internet]. Tropical medicine & international health : TM & IH. 2009. p. 504–11. Available from: http://www.ncbi.nlm.nih.gov/pubmed/19320869

Lim P, Alker AP, Khim N, Shah NK, Incardona S, Doung S, et al. Pfmdr1 copy number and arteminisin derivatives combination therapy failure in falciparum malaria in Cambodia. Malar J [Internet]. 2009 Jan [cited 2012 Aug 10];8:11. Available from: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2627910&tool=pmcentrez&rendertype=abstract

Vinayak S, Alam MT, Sem R, Shah NK, Susanti AI, Lim P, et al. Multiple genetic backgrounds of the amplified Plasmodium falciparum multidrug resistance (pfmdr1) gene and selective sweep of 184F mutation in Cambodia. J Infect Dis [Internet]. 2010 May 15 [cited 2014 Mar 7];201(10):1551–60. Available from: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=3096139&tool=pmcentrez&rendertype=abstract

Tanabe K, Zakeri S, Palacpac NMQ, Afsharpad M, Randrianarivelojosia M, Kaneko A, et al. Spontaneous mutations in the Plasmodium falciparum sarcoplasmic/ endoplasmic reticulum Ca2+-ATPase (PfATP6) gene among geographically widespread parasite populations unexposed to artemisinin-based combination therapies. Antimicrob Agents Chemother [Internet]. 2011 Jan [cited 2012 Aug 10];55(1):94–100. Available from: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=3019649&tool=pmcentrez&rendertype=abstract

Prajapati SK, Joshi H, Shalini S, Patarroyo M a, Suwanarusk R, Kumar A, et al. Plasmodium vivax lineages: geographical distribution, tandem repeat polymorphism, and phylogenetic relationship. Malar J [Internet]. BioMed Central Ltd; 2011 Jan [cited 2012 Aug 10];10(1):374. Available from: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=3258263&tool=pmcentrez&rendertype=abstract

Mayengue PI, Ndounga M, Malonga FV, Bitemo M, Ntoumi F. Genetic polymorphism of merozoite surface protein-1 and merozoite surface protein-2 in Plasmodium falciparum isolates from Brazzaville, Republic of Congo. Malar J [Internet]. BioMed Central Ltd; 2011 Jan [cited 2012 Aug 10];10(1):276. Available from: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=3195763&tool=pmcentrez&rendertype=abstract

Mallick PK, Joshi H, Valecha N, Sharma SK, Eapen A, Bhatt RM, et al. Mutant pfcrt “SVMNT” haplotype and wild type pfmdr1 “N86” are endemic in Plasmodium vivax dominated areas of India under high chloroquine exposure. Malar J [Internet]. BioMed Central Ltd; 2012 Jan [cited 2012 Aug 10];11(1):16. Available from: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=3283508&tool=pmcentrez&rendertype=abstract

Ongagna-Yhombi SY, Corstjens P, Geva E, Abrams WR, Barber C a, Malamud D, et al. Improved assay to detect Plasmodium falcip

Published
2019-03-11
How to Cite
1.
SUWANDI JF, ASMARA W, KUSNANTO H, SYAFRUDDIN D, SUPARGIYONO S. Efficacy of Artemisinin Base Combination Therapy and Genet-ic Diversity of Plasmodium falciparum from Uncomplicated Malaria Falciparum Patient in District of Pesawaran, Province of Lampung, Indonesia. Iran J Parasitol. 14(1):143-150.
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Original Article(s)