Tehran University of Medical Sciences Iranian Journal of Parasitology 1735-7020 14 1 2019 03 11 143 150 EN Jhons Fatriyadi SUWANDI Department of Microbiology and Parasitology, Faculty of Medicine, University of Lampung, Bandar Lampung, Indonesia Widya ASMARA Department of Microbiology, Faculty of Veterinary Medicine, Gadjah Mada University, Jogjakarta, Indonesia Hari KUSNANTO Department of Public Health, Faculty of Medicine, Public Health and Nursing, Gadjah Mada University, Jogjakarta, Indonesia Din SYAFRUDDIN Department of Parasitology, Faculty of Medicine, Hasanuddin University, Makasar, Indonesia Eijkman Institute for Molecular Biology, Jakarta, Indonesia Supargiyono SUPARGIYONO Department of Parasitology, Faculty of Medicine, Public Health and Nursing, Gadjah Mada University, Jogjakarta, Indonesia 2018 02 20 2018 10 15 Background: Malaria is an infectious disease caused by Plasmodium sp., that still prevalence in some part of Indonesia. District of Pesawaran is one of malaria endemic area in the Province of Lampung. The purpose of this study was to evaluate the efficacy of the ACT treatment in the District of Pesawaran Province of Lampung, Indonesia from Dec 2012 to Jul 2013 and the genetic variation of the Plasmodium falciparum also studied. Methods: This study was observational analytic study of falciparum malaria patients treated with ACT and primaquine (DHP-PQ and AAQ-PQ) at Hanura Primary Health Centre (Puskesmas). DNA isolation was done with QIAmp DNA Mini Kit. Amplification of PfMDR1, MSP1, and MSP2 genes was done with appropriate forward and reverse primer and procedures optimized first. PCR Product of PfMDR1 gene was prepared for sequencing. Data analysis was done with MEGA 6 software. Results: The results of this research are DHP-PQ effectiveness was still wellness among falciparum malaria patients in District of Pesawaran, Province of Lampung, Indonesia. There is Single-nucleotide mutation of N86Y of PfMDR1 gene. The dominant alleles found are MAD20 and 3D7 alleles with Multiplicity of Infection (MOI) are low. Conclusion: Therapy of DHP-PQ as an antimalarial falciparum in Pesawaran District, Lampung, Indonesia is still good. The genetic variation found was the SNP on the N86Y PfMDR1 gene, with dominant allele MAD20 and 3D7. https://ijpa.tums.ac.ir/index.php/ijpa/article/view/2003 https://ijpa.tums.ac.ir/index.php/ijpa/article/download/2003/923