New Method of Production and Characterization of Haemozo-in and Β-Haemozoin from Meccus longipennis

  • Liliana GONZÁLEZ-LINARES Department of Medicine, School of Biological and Health Sciences, Autonomous University of Hidalgo State, Hidalgo, México
  • Víctor Esteban REYES-CRUZ Department of Materials and Earth Sciences, School of Engineering and Basic Sciences, Autonomous University of Hidalgo State, Hidalgo, México
  • María Aurora VELOZ-RODRÍGUEZ Department of Materials and Earth Sciences, School of Engineering and Basic Sciences, Autonomous University of Hidalgo State, Hidalgo, México
  • Gustavo URBANO-REYES Department of Materials and Earth Sciences, School of Engineering and Basic Sciences, Autonomous University of Hidalgo State, Hidalgo, México
  • José Luis IMBERT-PALAFOX Department of Medicine, School of Biological and Health Sciences, Autonomous University of Hidalgo State, Hidalgo, México
  • José Angel COBOS-MURCIA Department of Materials and Earth Sciences, School of Engineering and Basic Sciences, Autonomous University of Hidalgo State, Hidalgo, México Mexican National Council for Science and Technology, México City, México
Keywords: Haemozoin, Meccus longipennis, Drug evaluations, β-haemozoin, Dimerization, Malaria

Abstract

Background: Understanding the significance of hemozoin (Hz) in the process through which Plasmodium is released from the heme group in the food vacuole during hemoglobin degradation, will allow the development of more effective drugs against malaria. Therefore, the development of methodologies to obtain Hz synthetically will facilitate an in vitro evaluation of new anti-malarial drugs. Methods: We present a methodology with good results to obtain Hz from fecal material of blood-sucking insects Meccus longipennis. The preparation of biological cultures of the parasite (Plasmodium) transmitter of the disease is not necessary. Results: The hemozoin molecule and its dimer were obtained using the method described and it was possible to validate a comparison with the positive and negative controls using different analytical techniques. Conclusion: The proposed method allows obtaining hemozoin and its dimer demonstrating equivalence with positive controls that demonstrate that the present procedure may be an alternative for the evaluation of antimalarial drugs.

References

Word Health Organization (WHO). World malaria report. WHO Library Cataloguing, ISBN: 978 92 4 156483 0; 2014

Sullivan DJ. Hemozoin: a biocrystal synthesized during the degradation of hemoglobin Biopolymers 9: Wiley online Library; 2005.

Fitch CD. Ferriprotoporphyrin IX, phospholipids and the antimalarial actions of quinoline drugs. Life sciences 2004; 74, 57–72

Bendrat K, Berger BJ, Cerami A. Haem polymerization in malaria. Nature 1995; 378: 138-9

Bohle DS, Dinnebier RE, Madsen SK, Stephens PW. Characterization of the products of the heme detoxification pathway in malarial late trophozoites by X-ray diffraction. J. Biol. Chem. 1997; 272: 713–6

Egan TJ. Haemozoin formation. Mol. Biochem. Parasitol 2008; 157, 127–36

Stiebler R, Hoang AN, Egan TJ, Wright DW, Oliveira MF. Increase on the initial soluble heme levels in acidic conditions is an important mechanism for spontaneous heme crystallization in vitro. PLoS ONE 5 2010

Egan TJ, Mavuso WW, Ncokazi KK. The mechanism of beta-hematin formation in acetate solution. Parallels between hemozoin formation and biomineralization processes. Biochem 2001; 40: 204–13

Jackson KE, Klonis N, Ferguson DJP, Adisa A, Dogovsky C, Tilley L. Food vacuole-associated lipid bodies and heterogeneous lipid environments in the malaria parasite, Plasmodium falciparum. Mol. Microbiol 2004; 54: 109–22

Pisciotta JM, Coppens I, Tripathi AK, Scholl PF, Shuman J, Bajad S. The role of neutral lipid nanospheres in Plasmodium falciparum haem crystallization. Biochem. J. 2007; 402: 197-204

Schwarzer E, Turrini F, Ulliers D, Giribaldi G, Ginsburg H, Arese PJ. Impairment of Macrophage Functions after Ingestion of Plasmodium s Erythrocytes or Isolated Malarial. Pigment. J. Exp. Med. 1992; 172: 1033-41

Arese P and Schwarzer E. Malarial pigment (haemozoin): a very active 'inert' substance. Ann. Trop. Med. Parasitol. 1997; 91: 501-16

Schwarzer E, Müller O, Arese P, Siems WG, Grune T. Increased levels of 4-hydroxynonenal in human monocytes fed with malarial pigment hemozoin. FEBS Lett 1996; 388: 119-22

Green MD, Xiao L, Dubb A. Formation of hydroxyeicosatetraenoic acids from hemozoin-catalyzed oxidation of arachidonic acid. Mol. Biochem. Parasitol. 1996; 83: 183-8

Schwarzer E, Ludwig P, Valente E, Arese P. 15(S)-hydroxyeicosatetraenoic acid (15-HETE), a product of arachidonic acid peroxidation, is an active component of hemozoin toxicity to monocytes. Parassitologia 1999; 41: 199-202

Oliveira MF, Silva JR, Dansa-Petretski M, De Souza W, Lins U and Braga CM. Haem detoxification by an insect. Nature 1999; 400: 517-8

Oliveira MF, D'Avila JC, Torres CR, Oliveira PL, Tempone AJ, Rumjanek FD. Haemozoin in Schistosoma mansoni. Mol. Biochem. Parasitol. 2000; 111: 217-21

Oliveira MF, Kycia SW, Gomez A, Kosar AJ, Bohle DS, Hempelmann E. Structural and morphological characterization of hemozoin produced by Schistosoma mansoni and Rhodnius prolixus. FEBS letters 2005; 579: 6010-6

Wood BR, Langford SJ, Cooke BM, Glenister FK, Lim J, McNaughton D. Raman imaging of hemozoin within the food vacuole of Plasmodium falciparum trophozoites. FEBS letters 2003; 554: 247-252

Thomas V, Góis A, Ritts B, Burke P, Hänscheid T, McDonnel G. A Novel Way to Grow Hemozoin-Like Crystals In Vitro and Its Use to Screen for Hemozoin Inhibiting Antimalarial Compounds. PLoS ONE 2012; 7

Ambele MA, Sewell BT, Cummings FR, Smith PJ, Egan TJ. Synthetic Hemozoin (β-Hematin) Crystals Nucleate at the Surface of Neutral Lipid Droplets that Control Their Sizes. Cryst. Growth Des. 2013; 13: 4442–52

Slater AF, Swiggard WJ, Orton BR, Flitter WD, Goldberg DE, Cerami A. An iron-carboxylate bond links the heme units of malaria pigment. Proc. Natl. Acad. Sci. USA 1991; 88: 325-9

Cole KA, Ziegler J, Evans CA, Wright DW. Metalloporphyrins inhibit b-hematin (hemozoin) formation. J. Inorg. Biochem. 2000; 78: 109-15

Wood BR, Langford SJ, Cooke BM, Lim J, Glenister FK, Duriska M. Resonance Raman spectroscopy reveals new insight into the electronic structure of beta-hematin and malaria pigment. J. Am. Chem. Soc. 2004; 126: 9233-9239

Pagola S, Stephens PW, Bohle DS, Kosar AD, Madsen SK. The structure of malaria pigment beta-haematin. Nature 2000; 404: 307-10

Marom N, Tkatchenko A, Kapishnikov S, Kronik L, Leiserowitz L. Structure and Formation of Synthetic Hemozoin: Insights From First-Principles Calculations. Cryst. Growth. Des. 2011; 11: 3332-41

De Villiers KA, Kaschula CH, Egan TJ, Marques HM. Speciation and structure of ferriprotoporphyrin IX in aqueous solution: spectroscopic and diffusion measurements demonstrate dimerization, but not -oxo dimer formation. J. Biol. Inorg. Chem. 2007; 12: 101-17

Egan TJ, Ross DC, Adams PA. Quinoline anti-malarial drugs inhibit spontaneous formation of -haematin (malaria pigment). FEBS Letters 1994; 352: 54–7

Davidson G., 2010 Chapter. 3: Vibrational spectra of transition element compound. En Spectroscopic Properties of Inorganic and Organometallic Compounds. Ed. The Royal Society of Chemistry; 2010

Bilia AR, Lazari D, Messori L, Taglioli V, Temperini C, Vincieri FF. Simple and rapid physico-chemical methods to examine action of antimalarial drugs with hemin Its application to Artemisia annua constituents. Life Sciences 2002; 70: 769-78

Majumdar S, Mehdi OK, Mitra S. Stability and characterization of Iron (III) and Iron (II) heme peptides encapsulated in aqueous detergent micelles: 1H NMR and UV-Vis spectroscopic studies. Inorganic. Chem. 1991; 30: 700–5

Goldberg DE, Slater AF, Cerami A, Henderson GB. Hemoglobin degradation in the malaria parasite Plasmodium falciparum: an ordered process in a unique organelle. Proc. Natl. Acad. Sci. 1990; 87: 2931-5.

Published
2019-03-10
How to Cite
1.
GONZÁLEZ-LINARES L, REYES-CRUZ VE, VELOZ-RODRÍGUEZ MA, URBANO-REYES G, IMBERT-PALAFOX JL, COBOS-MURCIA J A. New Method of Production and Characterization of Haemozo-in and Β-Haemozoin from Meccus longipennis. Iran J Parasitol. 14(1):59-7.
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Original Article(s)