Tehran University of Medical Sciences Iranian Journal of Parasitology 1735-7020 14 1 2019 03 10 59 67 EN Liliana GONZÁLEZ-LINARES Department of Medicine, School of Biological and Health Sciences, Autonomous University of Hidalgo State, Hidalgo, México Víctor Esteban REYES-CRUZ Department of Materials and Earth Sciences, School of Engineering and Basic Sciences, Autonomous University of Hidalgo State, Hidalgo, México María Aurora VELOZ-RODRÍGUEZ Department of Materials and Earth Sciences, School of Engineering and Basic Sciences, Autonomous University of Hidalgo State, Hidalgo, México Gustavo URBANO-REYES Department of Materials and Earth Sciences, School of Engineering and Basic Sciences, Autonomous University of Hidalgo State, Hidalgo, México José Luis IMBERT-PALAFOX Department of Medicine, School of Biological and Health Sciences, Autonomous University of Hidalgo State, Hidalgo, México José Angel COBOS-MURCIA Department of Materials and Earth Sciences, School of Engineering and Basic Sciences, Autonomous University of Hidalgo State, Hidalgo, México AND Mexican National Council for Science and Technology, México City, México 2017 09 28 2019 03 10 Background: Understanding the significance of hemozoin (Hz) in the process through which Plasmodium is released from the heme group in the food vacuole during hemoglobin degradation, will allow the development of more effective drugs against malaria. Therefore, the development of methodologies to obtain Hz synthetically will facilitate an in vitro evaluation of new anti-malarial drugs. Methods: We present a methodology with good results to obtain Hz from fecal material of blood-sucking insects Meccus longipennis. The preparation of biological cultures of the parasite (Plasmodium) transmitter of the disease is not necessary. Results: The hemozoin molecule and its dimer were obtained using the method described and it was possible to validate a comparison with the positive and negative controls using different analytical techniques. Conclusion: The proposed method allows obtaining hemozoin and its dimer demonstrating equivalence with positive controls that demonstrate that the present procedure may be an alternative for the evaluation of antimalarial drugs. https://ijpa.tums.ac.ir/index.php/ijpa/article/view/1804 https://ijpa.tums.ac.ir/index.php/ijpa/article/download/1804/913