Prevalence and Genotyping of Trichomonas vaginalis Infected to dsRNA Virus by PCR–Restriction Fragment Length Poly-morphism (RFLP)

  • Fariba ORUJZADEH Department of Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
  • Fatemeh TABATABAIE Department of Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
  • Khadijeh KHANALIHA Research Center of Pediatric Infectious Diseases, Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences, Tehran, Iran
  • Lame AKHLAGHI Department of Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
  • Farah BOKHARAEI-SALIM Department of Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
  • Soudabeh FALLAH Department of Clinical Biochemistry, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
  • Abdoulreza ESTEGHAMATI Research Center of Pediatric Infectious Diseases, Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences, Tehran, Iran
  • Hossein MASOUMI-ASL Research Center of Pediatric Infectious Diseases, Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences, Tehran, Iran
Keywords: Genotype, Actin, RFLP, Trichomonas vaginalis

Abstract

Background: Trichomonas vaginalis is a prevalent sexually transmitted infection cause trichomoniasis. In this study prevalence and genotype of Iranian isolates of T. vaginalis infected (dsRNA) viruses were evaluated by PCR-RFLP and obtained patterns were then confirmed by sequence analysis and genotype of these Iranian isolates confirmed again. Methods: Ten strains of T.vaginalis were collected from 1700 vaginal samples of women referred to hospitals associated with Iran University of Medical Sciences in Tehran, Iran during Feb 2016 to Jul 2017, evaluated in points of infection to T. vaginalis Virus (TVV-1) were used in a PCR-RFLP. All of ten isolates of T. vaginalis were examined by designed nested PCR for actin gene and then digestion patterns of three endonuclease enzymes of HindII, MseI and RsaI were evaluated and genotype of these isolates was defined. Results: By combination of fragments pattern of three enzymes of HindII, RsaI and MseI, three genotypes were found; six genotypes E, two genotypes G and two genotypes I. The most dominant genotypes were genotype E. Among four TVV infected isolates two genotype E, one genotype G and one genotype I were found, however among six uninfected T. vaginalis isolates to TVV-1, all of three genotypes were also found. Conclusion: Three genotypes E, G and I in T. vaginalis infected with dsRNA isolates were found, however, these three genotypes in T. vaginalis without virus were also observed. Further study is needed to evaluate genotypes of T. vaginalis, which infected virus in more great T. vaginalis population.

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Published
2019-06-19
How to Cite
1.
ORUJZADEH F, TABATABAIE F, KHANALIHA K, AKHLAGHI L, BOKHARAEI-SALIM F, FALLAH S, ESTEGHAMATI A, MASOUMI-ASL H. Prevalence and Genotyping of Trichomonas vaginalis Infected to dsRNA Virus by PCR–Restriction Fragment Length Poly-morphism (RFLP). Iran J Parasitol. 14(2):250-257.
Section
Original Article(s)