Short Communication

Genetic Diversity of Dihydropteroate synthetase Gene (dhps) Of Plasmodium vivax in Hormozgan Province, Iran


Background: The present study was formulated in order to determine pol­ymorphism of dihydropteroate synthetase gene (dhps) of Plasmodium vivax (P. vivax) in Hormozgan Province, southern Iran and mutations at codons 382, 383, 512, 553, and 585 associated with resistance of P. vivax to sulfadoxine.

Method: One-hundred eighteen isolates of P. vivax were prepared within 2007-2008 to determine dihydrofolate reductase-thymidylate synthase (dhfr-ts) gene. The isolates were determined in the study of genetic diversity of dihy­dropteroate synthetase gene (dhps) of P. vivax. The study was performed via PCR test and nucleotide sequencing.

Results: Of 118 blood samples infected by P. vivax, 46 and 72 samples be­longed to Minab and Jask, respectively. No mutation was detected at 5 target codons. However, among these 118 samples, three isolates (2.54%) were found to have a mutation at the new codon 421.

Conclusion: Since mutation was detected in dihydrofolate reductase (Pvdhfr) gene in the same samples but no mutation was found at five main codons of Pvdhps gene, it can be concluded that P. vivax, considering their mutations in Pvdhfr, is still susceptible to sulfadoxine and therefore, to fansidar in Hor­mozgan Province, Southern Iran.

Bright AT, Manary MJ, Tewhey R, Arango EM, Wang T, Schork NJ, et al. A high resolution case study of a patient with recurrent Plasmodi¬um vivax infections shows that relapses. PLoS Negl Trop Dis. 2014; 8(6):e2882.

Mendis K, Sina BJ, Marchesini P, Carter R. The neglected burden of Plasmodium vivax malaria. Am J Trop Med Hyg. 2001; 64: 97-106.

Raeisi A, Nikpoor F, Ranjbar Kahkha M, Faraji L. The trend of Malaria in I.R. Iran from 2002 to 2007. Hakim Res J. 2009; 12 (1): 35- 41. (In Persian)

Edrisian Gh, Rezaeian M, Ghorbani M, Keshavarz H, Moheballi. Medical protozoolo¬gy. Tehran University of Medical Sciences press, First edition; 2014 (In Persian).

Imwong M, Pukrittakayamee S, Looareesuwan S, Pasvol G, Poirreiz J, White NJ, et al. Associ¬ation of genetic mutations in Plasmodium vivax dhfr with resistance to sulfadoxine-pyrimeth-amine: geographical and clinical correlates. An-timicrob Agents Chemother. 2001; 45(11): 3122-3127.

Rungsihirunrat K, Sibley CH, Mungthin M, Na-Bangchang K. Geographical Distribution of Amino Acid Mutations in Plasmodium vivax DHFR and DHPS from Malaria Endemic Ar¬eas of Thailand. Am J Trop Med Hyg. 2008;78(3):462-467.

Barnadas C, Tichit M, Bouchier C, Ratsimba¬soa A, Randrianasolo L, Raherinjafy R, Jahevit¬ra M, Picot S, Menard D. Plasmodium vivax dhfr and dhps mutations in isolates from Madagascar and therapeutic response to sulphadoxine-pyrimethamine. Malaria J. 2008; 7(35):1-11.

Udomsangpetch R, Kaneko O, Chotivanich K, Sattabongkot J. Cultivation of Plasmodium vivax. Trends Parasitol. 2008; 24(2):85-88.

Yuan J, Cheng KC, Johnson RL, Huang R, Pattaradilokrat S, Liu A, Guha R, et al. Chemi¬cal genomic profiling for antimalarial therapies, response signatures, and molecular targets. Sci¬ence. 2011; 333(6043): 724–729.

Ferone R. Folate metabolism in malaria. Bulletin WHO. 1977;55 (2-3): 291-298.

Hawkins VN, Joshi H, Rungsihirunrat K, Na-Bangchang K, Sibley CH: Antifolates can have a role in the treatment of Plasmodium vivax. Trends Parasitol. 2007;23(5): 213-222.

Ter Kuile FO, Dolan G, Nosten F, et al. Halo¬fantrine versus mefloquine in treatment of mul¬tidrug-resistant falciparum malaria. Lancet. 1993;341:1044–1049.

de Pecoulas PE, Tahar R, Ouatas T, Maza-braud A, Basco LK. Sequence variations in the Plasmodium vivax dihydrofolate reductase-thymidylate synthase gene and their relation¬ship with pyrimethamine resistance. Mol Bio¬chem Parasitol. 1998; 92(2):265-273.

Schousboe ML, S Rajakaruna R, Salanti AC, Hapuarachchi H, NL Galappaththy G, C By¬gbjergI, et al. Island-wide diversity in single nu¬cleotide polymorphisms of the Plasmodium vivax dihydrofolate reductase and dihydropteroate synthetase genes in Sri-Lanka. Malaria J. 2007; 6:28: 1-6.

Sharifi Sarasiabi K. Thesis for Ph.D degree in medical parasitology.Genetic analysis of the dihidrofolate reductase - thymidylate synthase gene in Plasmodium vivax in Hormozgan prov¬ince, Iran. PhD thesis, Shahid Beheshti Univer¬sity of Medical sciences. 2009-2010 (In Persian).

Sharifi K, Haghighi A, Gachkar L, Kazemi B, Taghipour N, Hosseinzadeh N. Molecular Characterization of Dihydrofolate Reductase – Thymidylate Synthase Gene Concering Anti¬fulate Resistance of Plasmodium vivax. Iran J Parasitol. 2009; 4: 10-18.

Hamedi Y. Malaria drug resistance in Iran. Hormozgan Medical Journal, Hormozgan. 2006; 10(2): 93-99 (In Persian).

Young MD, Burgess RW. Pyrimethamine re¬sistance in Plasmodium vivax malaria. Bull World Health Organ. 1959; 20(1): 27–36.

Ahmed A, Bararia D, Vinayak S, Yameen M, Biswas S, Dev V. Plasmodium falciparum Isolates in India Exhibit a Progressive Increase in Mu¬tations Associated with Sulfadoxine-Pyrimethamine Resistance. Antimicrob Agents Chemother. 2004; 48(3):879-889.

Imwong M, Pukrittayakamee S, Cheng Q, Moore C, Looareesuwan S, Snounou G, et al. Limited Polymorphism in the Dihydrop¬teroateSynthetase Gene (dhps) of Plasmodium vivax Isolates from Thailand. Antimicrob Agents Chemother. 2005; 49:4393-4395.

Martha Sa J, Yamamoto MM, Fernandez-Becerra C, Ferreira de Azevedo M, Papakrivos J , Naude B , et al. Expression and function of pvcrt-o, a Plasmodium vivax or thologofpfcrt in Plasmodium falciparum and Dictyostelium dis¬coideum. Mol Biochem Parasitol. 2006; 150: 219–228.

Payne D. Did medicated salt hasten the spread of chloroquine resistance in Plasmodium falcipa¬rum? Parasitology Today.1988;4:112–115.

Auliff A, Wilson DW, Russell B, Gao Q, Chen N, Anh le N, Maguire J, Bell D,O’Neil MT, Cheng Q. Amino acid mutations in Plas¬modium vivax DHFR and DHPS from several geographical regions and susceptibility to anti¬folate drugs. Am J Trop Med Hyg. 2006; 75:617-621.

Rungsihirunrat K, Na-Bangchang K, Hawkins VN, Mungthin M, Sibley CH. Sensitivity to an¬tifolates and genetic analysis of Plasmodium vivax isolates from Thailand. Am J Trop Med Hyg. 2007; 76:1057-1065.

ZamanJ, Shahbazi A, Asgharzadeh M. Plasmo¬dium vivax dhfr Mutations among Isolates from Malarious Areas of Iran. Korean J Parasitol. 2011; 49:125-131.

Khatoon L, Baliraine FN, Bonizzoni M, Malik SA, Yan G. Prevalence of antimalarial drug re¬sistance mutations in Plasmodium vivax and P.falciparum from a malaria-endemic area of Pa¬kistan. Am J Trop Med Hyg. 2009; 81:525-528.

IssueVol 11 No 1 (2016) QRcode
SectionShort Communication(s)
Genetic diversity Dihydropteroate synthetase gene P. vivax Iran

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How to Cite
MAGHSOODLOORAD S, HAGHIGHI A, SHARIFI SARASIABI K, TAGHIPOOR N, HOSSEINZADEH N, GACHKAR L, NAZEMALHOSSEINI MOJARRAD E, MAGHSOODLOORAD E. Genetic Diversity of Dihydropteroate synthetase Gene (dhps) Of Plasmodium vivax in Hormozgan Province, Iran. Iran J Parasitol. 11(1):98-103.