DNA Sequence Polymorphism of the Lactate Dehydrogenase Genefrom Iranian Plasmodium vivax and Plasmodium falciparum Isolates
-
Daniel GETACHER FELEKE
,
Mehdi NATEGHPOUR
,
Afsaneh MOTEVALLI HAGHI
,
Homa HAJJARAN
,
Leila FARIVAR
,
Mehdi MOHEBALI
,
Reza RAOOFIAN
Abstract
Background: Parasite lactate dehydrogenase (pLDH) is extensively employed as malaria rapid diagnostic tests (RDTs). Moreover, it is a well-known drug target candidate. However, the genetic diversity of this gene might influence performance of RDT kits and its drug target candidacy. This study aimed to determine polymorphism of pLDH gene from Iranian isolates of P. vivax and P. falciparum.
Methods: Genomic DNA was extracted from whole blood of microscopically confirmed P. vivax and P. falciparum infected patients. pLDH gene of P. falciparum and P. vivax was amplified using conventional PCR from 43 symptomatic malaria patients from Sistan and Baluchistan Province, Southeast Iran from 2012 to 2013.
Results: Sequence analysis of 15 P. vivax LDH showed fourteen had 100% identity with P. vivax Sal-1 and Belem strains. Two nucleotide substitutions were detected with only one resulted in amino acid change. Analysis of P. falciparum LDH sequences showed six of the seven sequences had 100% homology with P. falciparum 3D7 and Mzr-1. Moreover, PfLDH displayed three nucleotide changes that resulted in changing only one amino acid. PvLDH and PfLDH showed 75%-76% nucleotide and 90.4%-90.76% amino acid homology.
Conclusion: pLDH gene from Iranian P. falciparum and P. vivax isolates displayed 98.8-100% homology with 1-3 nucleotide substitutions. This indicated this gene was relatively conserved. Additional studies can be done weather this genetic variation can influence the performance of pLDH based RDTs or not.
Bousema T, Drakeley C. Epidemiology and infectivity of Plasmodium falciparum and Plasmodium vivax gametocytes in relation to malaria control and elimination. Clin Microbiol Rev. 2011;24:377-410.
WHO. World malaria report. 2013.
Guerra CA, Howes RE, Patil AP, Gething PW, Van Boeckel TP, Temperley WH, Kabaria CW, Tatem AJ, Manh BH, Elyazar IR. The international limits and population at risk of Plasmodium vivax transmission in 2009. PLoS NTD. 2010;4:e774.
Lee SJ, Seo E, Cho Y. Proposal for a new therapy for drug-resistant malaria using Plasmodium synthetic lethality inference. Int J Parasitol. 2013;3:119-128.
Fekri S, Vatandoost H, Daryanavard A, Shahi M, Safari R, Raeisi A, Omar AS, Sharif M, Azizi A, Ali AA. Malaria situation in an endemic area, southeastern Iran. J Arthropod-Borne Dis. 2013;8:82.
Haghi A, Shahrbabak S. Sequence analysis of different domains of Plasmodium vivax apical membrane antigen (pvama-1 gene) locus in iran. Iran J Parasitol. 2012;7(1):26-31.
Aregawi M, Cibulskis RE, Otten M, Williams R. World malaria report 2009. World Health Organization; 2009.
Tjitra E, Suprianto S, Dyer M, Currie BJ, Anstey NM. Field evaluation of theictmalariapf/pvimmunochromatographic test for detection of Plasmodium falciparum and Plasmodium vivax in patients with a presumptive clinical diagnosis of malaria in eastern Indonesia. J Clin Microbiol. 1999;37:2412-2417.
Norgan AP, Arguello HE, Sloan LM, Fernholz EC, Pritt BS. A method for reducing the sloughing of thick blood films for malaria diagnosis. Malaria J. 2013;12:231.
Tangpukdee N, Duangdee C, Wilairatana P, Krudsood S. Malaria diagnosis: A brief review. The Korean J Parasitol. 2009;47:93-102.
Smith P. A rapid dipstick antigen capture assay for the diagnosis of falciparum malaria. Indian J Pediatrics. 1996; 74(1): 47–54.
Gbotosho GO, Happi CT, Folarin O, Keyamo O, Sowunmi A, Oduola AM. Rapid detection of lactate dehydrogenase and genotyping of Plasmodium falciparum in saliva of children with acute uncomplicated malaria. Am J Trop Med Hyg. 2010;83:496.
Jang JW, Cho CH, Han ET, An S, Lim CS. Pldh level of clinically isolated Plasmodium vivax and detection limit of pldh based malaria rapid diagnostic test. Malar J. 2013;12:181.
Iqbal J SA, Jameel M, Hira P.R. Persistent histidine-rich protein 2, parasite lactate dehydrogenase, and panmalarial antigen reactivity after clearance of Plasmodium falciparum mono infection. J Clin Microbiol. 2004; 42(9): 4237–4241.
Garba I, Ubom G. Total serum lactate dehydrogenase activity in acute Plasmodium falciparum malaria infection. Singapore Med J. 2005;46:632.
Gomez MS, Piper RC, Hunsaker LA, Royer RE, Deck LM, Makler MT, Vander Jagt DL. Substrate and cofactor specificity and selective inhibition of lactate dehydrogenase from the malarial parasite P. falciparum. Molecular Biochem Parasitol. 1997;90:235-246.
Atchade PS, Doderer-Lang C, Chabi N, Perrotey S, Abdelrahman T, Akpovi CD, Anani L, Bigot A, Sanni A, Candolfi E. Is a Plasmodium lactate dehydrogenase (pldh) enzyme-linked immunosorbent (elisa)-based assay a valid tool for detecting risky malaria blood donations in africa? Malar J. 2013;12:279.
Mariette N, Barnadas C, Bouchier C, Tichit M, Ménard D. Country-wide assessment of the genetic polymorphism in Plasmodium falciparum and Plasmodium vivax antigens detected with rapid diagnostic tests for malaria. Malar J. 2008;7:219.
Hu MJ. A study of polymorphism of lactate dehydrogenase(ldh) gene of Plasmodium vivax from Jianghuai regin. Pathogen Biol. 2012;Master
Lv G, Li J-J, Zhao S-Y, Lu Y-J, Fan Z-G. [molecular cloning and sequence analysis of lactate dehydrogenase gene from Plasmodium vivax hainan isolate]. Chinese J Parasito Parasitic Dis. 2010;28:72-74.
Hu M, Fang Q, Wu S, Tang B, Zhang J, Jiao Y, Xia H, Shen J. [sequence homology analysis on lactate dehydrogenase (ldh) of Plasmodium vivax anhui isolates]. Chinese J Parasito Parasitic Dis. 2013;31:148-150.
Talman AM, Duval L, Legrand E, Hubert V, Yen S, Bell D, Le Bras J, Ariey F, Houze S. Evaluation of the intra-and inter-specific genetic variability of Plasmodium lactate dehydrogenase. Malaria J. 2007;6:140.
Shin H-I, Kim J-Y, Lee W-J, Sohn Y, Lee S-W, Kang Y-J, Lee H-W. Polymorphism of the parasite lactate dehydrogenase gene from Plasmodium vivax korean isolates. Malar J. 2013;12:166.
Jiang L, Wang Z, Ma X, Zhang X, Cai L. [comparative analysis of nucleotide sequences of lactate dehydrogenase (ldh) gene and ldh epitopes of Plasmodium vivax and Plasmodium falciparum]. Chinese J Parasito Parasitic Dis. 2010;28:103-107.
Hu MJ. A study of polymorphism of lactate dehydrogenase(ldh) gene of Plasmodium vivax from Jianghuai regin. Pathogen Biol. 2012. MSc Thesis.
Ali M, Hidayatullah TA, Alimuddin Z, Sabrina Y. Sequence diversity of pfmdr1 and sequence conserve of pldh in Plasmodium falciparum from Indonesia: Its implications on designing a novel antimalarial drug with less prone to resistance. Iran J Parasitol 2013;8 (4):522-9.
Akbulut E, Celık V, Balik DT. [Comparative analysis at the nucleotide level of the genes encoding the lactate dehydrogenase enzyme of Plasmodium vivax and Plasmodium falciparum.]. Acta Parasitologica Turcica. 2004;29:149-153.
Turgut-Balik D, Akbulut E, Shoemark DK, Celik V, Moreton KM, Sessions RB, Holbrook JJ, Brady RL. Cloning, sequence and expression of the lactate dehydrogenase gene from the human malaria parasite, Plasmodium vivax. Biotechnol lett. 2004;26:1051-1055.
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| Issue | Vol 10 No 4 (2015) | |
| Section | Original Article(s) | |
| Keywords | ||
| Iran Gene polymorphism Lactate Dehydrogenase gene P. falciparum P. vivax | ||
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