The Ultrastructural Effects of Sulfachloropyrazine on Toxoplasma gondii Tachyzoites
Abstract
Background: Toxoplasmosis is one of the most common parasitic infections of humans and other mammals. This study was aimed to understand the mechanism of action of veterinary medicine-sulfachloropyrazine (SPZ, 99.97%) against Toxoplasma gondii.
Methods: T. gondii tachyzoites were soaked in PBS (as a control) or SPZ (250 mg/mL) for 2 h at 37 °C. After being processed, any ultrastructural changes of the tachyzoites that had occurred were observed by Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM).
Results: The tachyzoites from control groups with a uniform size had a smooth surface and intact cell or nuclear membranes. In addition, an oval-shaped nucleus, conoids and micronemes were also observed. By contrast, many parasites from the SPZ-treated groups were detrimentally affected by the treatment. Some appeared to be of the vacuolization in their cytoplasm, with the substantial re-duction in the number of dense granules and the blur of some organelles.
Conclusion: The morphology and ultrastructure of tachyzoites can be affected significantly by SPZ, which might kill the parasite by inhibiting its energy metabolism, inducing apoptosis and damaging its structure. The study provides an experimental basis for further study on the mechanism of SPZ against T. gondii.
Roberts T, Murrell KD, Marks S. Economic losses caused by foodborne parasitic disease. Parasitol Today. 1994; 10(11):419-423.
Van Der Puije WNA, Bosompema KM, Cana-coob EA,Wastlingc JM, Akanmori BD. The prevalence of anti-Toxoplasma gondii antibodies in Ghanaian sheep and goats. Acta Tropica. 2000; 76(1):21-26.
Buxton D. Protozoan infections (Toxoplasma gondii, Neospora caninum and Sarcocystis spp.) in sheep and goats: recent advances. Vet Res. 1998; 29:289-310.
Dubey JP. Strategies to reduce transmission of Toxoplasma gondii to animals and humans. Vet Parasitol. 1996; 64(1-2):65-70.
Freyre A, Bonino J, Falcón J, Castells D, Cor-rea O, Casaretto A. The incidence and eco-nomic significance of ovine toxoplasmosis in Uruguay. Vet Parasitol. 1997; 73(1-2):13-15.
Chio LC, Bolyard LA, Nasr M, Queener SF. Identification of a class of sulfonamides highly active against dihydropteroate synthase from Toxoplasma gondii, Pneumocystis carinii, and Myco-bacterium avium. Antimicrob Agents Ch. 1996; 40:727–733.
Dubey JP, Shen SK. Rat model of congenital toxoplasmosis. Infect Immun. 1991; 59(9):3301-3302.
Johny S, Merisko A, Whitman DW. Efficacy of eleven antimicrobials against a gregarine parasite (Apicomplexa: Protozoa). Ann Clin Microbiol Antimicrob. 2007; 6:15.
Laczay P, Vörös G, Semjén G. Comparative studies on the efficacy of sulphachloropyrazine and toltrazuril for the treatment of caecal coccidiosis in chickens. Int J Parasitol. 1995; 25(6):753-756.
Penev PM, Lozanov L. Action of sodium sul-fachloropyrazine (ESB3) on the endogenous development of Eimeria tenella in the experi-mental infestation of chickens. Vet Med Nauki. 1983; 20(5-6):72-79.
Anderson NG, Alpaugh WC, Baughn CO. Effect of sulfachloropyrazine in the drinking water of chickens infected experimentally with fowl cholera. Avian Dis.1974; 18(3): 410-415.
Tarozzi F. Combination of anticoccidial drugs: experimental studies of the efficacy of the sul-fachloropyrazine-diaveridine-ethopabate com-bination in the prevention of avian coccidiosis. Folia Vet Lat.1974; 4(2):275-286.
Huang B, Zhu SH, Zeng YB, Dong H, Jiang LL, Han HY, Li Y, Zhao QP, Ping XQ, Cheng J. Evaluation of the efficacy of six drugs against acute murine toxoplasmosis. Chin J Anim Infect Dis. 2010; 18(3):45-50 (in Chi-nese).
Zeng YB, Zhu SH, Dong H, Hong YH, Jiang LL, Wang Q, Cheng J, Zhao QP, Ma WJ, Huang B. Great efficacy of sulfachloropyra-zine-sodium against acute murine Toxoplas-mosis. Asian Pac J Trop Biomed. 2012; 2(1):70-75.
Aikawa M. Parasitological review: Plasmodium: The fine structure of malarial parasites. Exp Parasitol. 1971; 30(2):284-320.
Stettler M, Fink R, Walker M, Walker M, Gottstein B, Geary TG, Rossignol JF, Hemphill A. In vitro parasiticidal effect of nitazoxanide against Echinococcus multilocularis metacestodes. Antimicrob Agents Ch. 2003; 47(2):467-474.
Dubremetz JF, Ferguson DJP. The role played by electron microscopy in advancing our un-derstanding of Toxoplasma gondii and other api-complexans. Int J Parasitol. 2009; 39(8):883-893.
Zhou BH, Wang HW, Xue FQ, Wang XY, Fei CZ, Wang M, Zhang T, Yao XJ, He PY. Ef-fects of diclazuril on apoptosis and mitochon-drial transmembrane potential in second-generation merozoites of Eimeria tenella. Vet Parasitol. 2010; 168(3-4):217-222.
Zeng YB, Zhu SH, Han HY, Dong H, Jiang LL, Zhao QP, Ma WJ, Cheng J, Huang B. Ef-ficacy of different kinds of drugs against acute murine toxoplasmosis. Chin J Zoonoses. 2011; 27(4):316-319 (in Chinese).
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| Issue | Vol 8 No 1 (2013) | |
| Section | Articles | |
| Keywords | ||
| Sulfachloropyrazine Tachyzoites Toxoplasma gondii Ultrastructure | ||
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