Geographical Distribution of Leishmania Species of Human Cutaneous Leishmaniasis in Fars Province, Southern Iran
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Abstract
Background: The goal of this study was to know the identity of Leishmania species responsible of cutaneous leishmaniasis (CL) in Fars Province, southern Iran.
Methods: Five counties of Shiraz, Firouz Abad, Ghir-Karzin, Farashband and Larestan were pros-pected. Forty-four patients exhibiting cutaneous lesions were selected. Samples collected on skin le-sions were examined both microscopically (after Giemsa staining) and molecularly (after PCR-RFLP).
Results: On the 44 examined patients, 39 exhibit Leishmania sp. by microscopical examination, all confirmed by PCR. For five patients with negative microscopical examination, PCR was positive for three of them. Among these 42 positive samples, 3 (7%) were infected by L. tropica and 39 (93%) by L. major.
Conclusions: Leishmania major is the most prevalent species in prospected area and L. tropica occurs in Shiraz and Ghir-Karzin counties.
Camargo LB, Langoni H. Impact of leishma-niasis on public health. J Venom Anim Toxins incl Trop Dis. 2006; 12 (4): 527-548.
Dey A, Singh S. Transfusion transmitted leish-maniasis: A case report and review of literature. Indian J Med Mic. 2006; 24(3):165-170.
Desjeux, P. Leishmaniasis: current situation and new perspectives, Comparative Immunol-ogy. J Microbiol Infect Dis. 2004; 27(5): 305–318.
Oshaghi MA, Rasolian M, Shirzadi MR, Moh-tarami F, Doosti S. First report on isolation of Leishmania tropica from sandflies of a classical urban cutaneous leishmaniasis focus in south-ern Iran. Exp Parasitol. 2010; 126(4): 445–450.
Nadim A, Seyedi-Rashti MA. A brief review of the epidemiology of various types of leish-maniasis in Iran. Acta Med Iran. 1971; 14(1): 99-106. 6. Yaghoobi-Ershadi MR, Javadian E, Tahvildare-Bidruni GH. Leishmania major MON-26 isolated from naturally infected Phle-botomus papatasi (Diptera: Psychodidae) in Is-fahan Province, Iran. Acta Trop. 1995; 59(4): 279-282.
Mohebali M, Javadian E, Yaghoobi-Ershadi MR, Akhavan AA, Hajjaran H, Abaei MR. Characterization of Leishmania infection in ro-dents from endemic areas of the Islamic Re-public of Iran. East Mediterr Health J. 2004; 10(4): 591-599.
Javadian E, Dehestani M, Nadim A, Rassi Y, Tahvildare-Bidruni GH, Seyedi-Rashti MA, Shadmehr A. Confirmation of Tatera indica (Rodentia: Gerbilidae) as the main reservoir host of zoonotic cutaneous leishmaniasis in the west of Iran. Iranian J Publ Health. 1998; 27(2): 55-60.
Nekouie H, Assmar M, Razavi MR, Naddaf SR. A study on Leishmania infection rate among Phlebotomus spp. collected from Abar-dejh district, Iran. Iranian J Vet R. 2006; 7(4): 77-81.
Sharifi I, Daneshvar H. The prevalence of visceral leishmaniasis in suspected canine reservoirs in Southern Iran. Iranian Med Sci. 1994; 21(4): 130-134.
Mahmoudvand H, Mohebali M, Sharifi I, Keshavarz H, Hajjaran H, Akhoundi B, Jahanbakhsh S, Zarean M, Javadi A. Epi-demiological Aspects of Visceral Leis-hmaniasis in Baft District, Kerman Province, Southeast of Iran. Iranian J Parasitol. 2011; 6(1):1-11.
Sharifi F, Sharifi I, Zarean M, Hakimi Parizi M, Aflatoonian MR, Fasihi Harandi M, Zahmatkesh R, Mashayekhi M, Ker-manizadeh AR. Spatial Distribution and Molecular Identification of Leishmania Species from Endemic Foci of South-Eastern Iran. Iranian J Parasitol. 2012; 7(1):45-52.
Iran Ministry of Health & Medical Education (IMHME), 2010. Official Report of Leishmania cases in Iran.
Hatam GR, Rezanezhad H, Motazedian MH, Sarkari B. In vitro infectivity of Leishmania ma-jor isolated from patients with different clinical forms of cutaneous leishmaniasis and its asso-ciation with parasite zymodems. Iranian J Parasitol. 2009; 4(3): 52-60.
Razmjou S, Hejazi H, Motazedian MH, Baghaei M, Emamy M, Kalantary M. A new focus of zoonotic cutaneous leishmaniasis in Shiraz, Iran. Trans R Soc Trop Med Hyg. 2009; 103 (7): 727-30.
Pourmohammadi B, Motazedian MH, Hatam GR, Kalantari M, Habibi P, Sarkari B. Com-parison of Three Methods for Diagnosis of Cutaneous Leishmaniasis. Iranian J Parasitol. 2010; 5(4):1-8.
Rassi Y, Javadian E, Jalali M, Motazedian MH, Vatndoost H. Investigation on Zoonotic Cu-taneous Leishmaniasis, Southern Iran. Iranian J Publ Health. 2004; 33(1):31-35.
Davami MH, Motazedian MH, Sarkari B. The changing profile of cutaneous leishmaniasis in a focus of the disease in Jahrom district, southern Iran. Ann Trop Med Parasitol. 2010; 104(5): 377-382.
Evans D (1989). Hand book on isolation. Characterization and Cryopreservation of Leishmania. First ed. UNDP/ WORLD BANK/WHO.
World Health Organization (1991). Basic la-boratory Methods in Medical Parasitology. First ed. WHO, Geneva.
Kelly JM (1993). Isolation of RNA & DNA from Leishmania. In: Method in Molecular Bi-ology. Ed, Hyde JE. Human a Press Newjersey USA.
Schönian G, Nasereddin A, Dinse N, Schweynoh C, Schalling HD, Presbe W, Jaffe C. PCR diagnosis and characterization of Leishmania in local and imported clinical sam-ples. Diag Microbiol Infect. 2003; 47(1):349-58.
Hajjaran H, Vasigheh F, Mohebali M, Rezaei s, Mamishi S, Charedar S. Direct diagnosis of Leishmania species on serosity materials punc-tured from cutaneous leishmaniasis patients using PCR-RFLP. J Clin Lab Anal. 2011; 25(4):20-24.
Lainson R, Shaw JJ. Evolution, classification and geographical distribution. In: W. Peters, & R. Killick-Kendrick (Eds), The Leishmaniases in Biology and Medicine. Orlando: Academic Press; 1987. pp 1–120.
Pearson RD, De Queiroz Sousa A, Jeronimo SMB. Leishmania Species: Visceral (Kala-Azar), Cutaneous and Mucosal Leishmaniasis. In: G. L. Mandell, J. E. Bennett, & R. Dolin (Eds), Principles and practice of infectious diseases. New York: Churchill Livingstone; (2001). pp 2831–2845.
Volpini AC, Passos VMA, Oliveira GC, Romanha AJ. PCR-RFLP to identify Leishmania (Viannia) braziliensis and L. (Leishmania) amazonensis causing American cu-taneous leishmaniasis. Acta Trop. 2004; 90(2): 31-7.
Al-Jawabreh A, Schnur LF, Nasereddin A, Schwenkenbecher JM, Abdeen Z, Barghuthy F, Khanfar H, Presber W, Schonian G. The recent emergence of Leishmania tropica in Jeri-cho (A'riha) and its environs, a classical focus of L. major. Trop Med Int Health. 2004; 9(7):812-16.
Gadisa E, Genetu A, Kuru T, Jirata D, Dagne K, Aseffa A, Gedamu L. Leishmania (Kinetoplastida): Species typing with isoenzyme and PCR-RFLP from Cutaneous Leishmaniasis patients in Ethiopia. Exp Parasitol. 2007; 115(4): 339-43.
Sinha PK, Pandey K, Bhattacharya SK. Diag-nosis & management of leishmania/HIV co-infection. Indian J Med Res. 2005; 121(4): 407-414.
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| Issue | Vol 8 No 1 (2013) | |
| Section | Articles | |
| Keywords | ||
| Human Iran Leishmania major Leishmania tropica PCR-RFLP | ||
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