Evaluation of Cross Immunity and Histopathological Findings in Experimentally Infected BALB/c Mice with Neospora caninum and Besnoitia caprae
-
M Namavari
,
A Oryan
,
F Namazi
,
M Kargar
,
M Mansourian
,
A Rahimian
,
Y Tahamtan
Abstract
Background: Caprine besnoitiosis is an economically important disease of goats. Neospora caninum, another coccidian parasite of worldwide distribution, infects several animal species and is a major cause of abortion in cattle. Combined infections of N. caninum and Besnoitia caprae can occur in geo-graphical areas endemic for both species of parasite in goats. This experiment was conducted to in-vestigate the possible cross-immunity between these two infections in experimentally infected BALB/c mice.
Methods: Forty BALB/c mice were divided into four equal groups. The mice of Groups 1 and 4 were inoculated with 1×106 live virulent tachyzoites of N. caninum (NC-1), while animals of Groups 2 and 3 were inoculated with sterile tissue culture medium. Each mouse in Groups 1 and 2 was chal-lenged 28 days later with 1×106 live virulent bradyzoites of B. Caprae (BC-1).
Results: Following the challenge, the mice in Groups 1 and 2 showed 100% morbidity and 100% mortality within 9 days post infection, while all the animals of Groups 3 and 4 remained alive. The dead animals were necropsied. The survivors (mice in Group 3 and 4) were euthanized 9 days after inoculation and the gross and histopathological lesions in different organs were investigated.
Conclusion: Immunization and challenge experiments with lethal dose of B. caprae in the highly sus-ceptible BALB/c mice showed no cross-protection between N. caninum and B. caprae.
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| Issue | Vol 8 No 1 (2013) | |
| Section | Articles | |
| Keywords | ||
| BALB/c mice Besnoitia caprae Cross reaction Neospora caninum | ||
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