Endoparasites of Stray Dogs in Mashhad, Khorasan Razavi Province, Northeast Iran with Special Reference to Zoonotic Parasites
-
Amir Adinezadeh
,
Eshrat Beigom Kia
,
Mehdi Mohebali
,
Saideh Shojaee
,
Mohammad Bagher Rokni
,
Zabihollah Zarei
,
Golamreza Mowlavi
Abstract
Background: To find out different species of helminthes and blood/tissue pro-tozoan parasites of stray dogs and their potential role for transmission of zoonotic species to human in Mashhad, Khorasan Razavi Province, northeast Iran, during 2008-2009.
Methods: Totally, 100 stray dogs were selected among Mashhad municipal collec-tion from different sites of the city. Internal organs were examined for any para-sites. Helminthes were identified based on morphological characteristics. Smears prepared from peripheral blood as well as liver, spleen and any skin lesion were stained by Giemsa and examined microscopically. Samples obtained from spleen were aseptically cultured in three culture media including NNN, Schneider’s Dro-sophila (HIMEDIA) and RPMI1640 (GIBCO) for isolation of Leishmania spp. The titer of anti-Leishmania and anti-Toxoplasma antibodies were measured by direct ag-glutination test (DAT) and indirect fluorescent antibody test (IFAT), respectively.
Results: 84% of dogs were infected at least with one species of intestinal hel-minthes. The species of parasites and rate of infection were as follows: Taenia hydatigena (61%), Dipylidium caninum (46%), Mesocestoides lineatus (19%), Echinococcus granulosus (10%), Toxascaris leonina (53%) and Toxocara canis (7%). Anti-Leishmania antibodies were detected by DAT in 8 dogs (8%) at 1:320 titers and higher. Forty seven dogs (47%) showed anti-Toxoplasma titer at 1:10 and 17 (17%) showed titer of ≥1:100. No blood parasites were found in prepared blood smears.
Conclusion: The high rate of parasitic infection and presence of zoonotic species especially E. granulosus and T. canis emphasizes the risk of diseases spread in urban areas by stray dogs
Craig PS, Rogan MT, Allan JC. Detection, screening and community epidemiology of taeniid cestode zoonoses: cystic ech-inococcosis, alveolar echinococcosis and neu-rocysticercosis. Adv Parasitol. 1996; 38: 169-250.
Dalimi A, Motamedi G, Hosseini M, Mohammadian B, Malaki H, Ghamari Z, Ghaffari Far, F. Echinococcosis/hydatidosis in western Iran. Vet Parasitol. 2002; 105: 161-171.
Razmi GR, Sardari K, Kamrani AR. Preva-lence of Echinococcus granulosus and other intes-tinal helminths of stray dogs in Mashhad area, Iran Arch Razi Ins. 2006; 61: 143-148.
Eslami A, Ranjbar-Bahadori Sh, Meshgi B, Dehghan M, Bokaie S. Helminth Infections of Stray Dogs from Garmsar, Semnan Prov-ince, Central Iran. Iran J Parasitol. 2010;5(4) : 37-41.
Sadjjadi SM. Present situation of echinococcosis in the Middle East and Ara-bic North Africa. Parasitol Int. 2006; 55 Suppl:S197-202.
Rokni MB. Echinococcosis/hydatidosis in Iran. Iran J Parasitol. 2009;4:1–16.
Eslami A. Veterinary Helminthology, Vol III. Nematoda and Acanthocephala. 2nd ed. Teh-ran University Publications, 1997 (in persion).
Mizgajska-Wiktor H, Uga S. Exposure and Environmental Contamination. In: Holland CV, Smith HV, editors. Toxocara: The Enig-matic Parasite. CABI Publishing, 2006.
Rokni M B. The present status of human helminthic diseases in Iran. Annal Trop Med Parasitol. 2008; 102 (4):283-295.
World Health Organization. Control of leishmaniases. WHO Expert Committee, Geneva. 2010. Technical report series 793.
Available: http://en.wikipedia.org/wiki/Mashhad
Mohebali M, Hajjaran H, Hamzavi Y, Mobedi I, Arshi S, et al. Epidemiological as-pects of canine visceral leishmaniasis in the Islamic Republic of Iran. Vet Parasitol. . 2005;129: 243-251.
Mohebali M, Edrissian Gh H, Shirzadi M R, Akhoundi B, Hajjaran H, et al. An observa-tional study on the current distribution of vis-ceral leishmaniasis in different geographical zones of Iran and implication to health policy. Travel Med Infect Dis. 2011 ; 9: 67-74.
Voller A. O’Neill, P. Immunofluorescence method suitable for large-scale application to malaria. Bulletin of the World Health Organ-ization. 1971; 45: 524-529.
Deplazes P, Eckert J. Diagnosis of the Echinococcus multilocularis infection in final hosts. Appl Parasitol. 1996; 37:245–252.
Khalil LF, Bray RA, Jones A. Keys to the Cestode Parasites of Vertebrates. CABI Pub-lishing, 1994.
Garcenea M, Gómez MS, Torres J. Preva-lence of intestinal parasites in shelter dogs and cats in the metropolitan area of Barcelo-na (Spain). Acta Parasitologica. 2009; 54(1), 73-77.
Gillespie SH, Pearson RD. Principles and Practice of Clinical Parasitology. John Wiley & Sons, Ltd; 2001.
Harith A, Salappendel RJ, Reiter I, Knapen F, Korte P, et al. Application of a direct aggluti-nation test for detection of specific anti-Leishmania antibodies in the canine reservoir. J Clin Microbiol. 1989; 27: 2252–2257.
Edrissian Gh H, Hajjaran H., Mohebali M, Soleimanzadeh G, Bokaei S . Application and evaluation of direct agglutination test in sero-diagnosis of visceral leishmaniasis in man and canine reservoirs in Iran. Iranian J Med Sci. 1996 ; 21: 119-124.
Boelaert M, El Safi S, Jacquet D, Muynck A, Stuyft PV, Ray D. Operational validation of direct agglutination test for diagnosis of vis-ceral leishmaniasis. Am J Trop Med Hyg. 1996; 60: 126–134.
Moreno J, Alvar J. Canine leishmaniasis: epi-demiological risk and the experimental mod-el. Trend Parasitol. 2002; 18: 399–405.
Gradoni L, Pozio E, Bettini S, Gramiccia M. Leishmaniasis in Tuscany (Italy).III. The prevalence of canine Leishmaniasis in two foci of Grosseto Province. Trans R Soc Trop Med Hyg. 1980;74: 421–422.
Lanotte G, Rioux JA, Perieres J, Vollhardt Y. Ecologie, des Leishmanioses dans le Sud de la France. 10. Les formes evolutives de la leishmaniose visceral canine. Elaboration d’une typologie bio-clinique a finalite epide-miologique. Ann Parasitol Hum Comp. 1979;54: 277–295.
Molina R, Amela C, Nieto J, San Andre ASM, Gonzalez F. Infectivity of dogs natu-rally infected with Leishmania infantum to col-onized Phlebotomus perniciosus. Trans R Soc Trop Med Hyg.1994; 88: 491–493.
Alvar J, Molina R, San Andre AS, Tesouro M, Nieto J, Vitutia M, Gonzalez, F, Boggio J. Rodriguez F, Sainz A, Escacena C. Canine leishmaniasis: clinical, parasitological and en-tomological follow-up after chemotherapy. Ann Trop Med Parasitol. 1994; 88: 371–378.
Moshfe A, Mohebali M, Edrissian Gh H, Zarei Z, Akhoundi B, et al. Canine visceral leishmaniasis: asymptomatic infected dogs as a source of Leishmania infantum infection. Acta Trop. 2009; 112: 101-115.
Mellor HE. Navigation of Lutzomyia longipalpis (Diptera: Psychodidae) under dusk or starlight conditions. B Entomol Res. 2003; 93 (4): 315-322.
Dubey JP, Lappin M. Toxoplasmosis and Neosporosis. In: Green C. editor. Infectious diseases of the dog and cat. New York; 2006 P. 2 754-2775.
Dubey JP.Toxoplasmosis of animals and humans.CRC press. 2010. Second edition.
Lin DS. Seroprevalence of Toxoplasma gondii in privately – owned dogs in Taiwan. Preven-tive Vet Med. 1998;35(1): 21-27.
Lee JY, Lee SE, Lee EG, Song KH. Nested PCR-based detection of Toxoplasma gondii in German shepherd dogs and stray cats in South Korea. Res Vet Sci.2008 :85:125-127 33. Ghorbani M, Hafizi A, Shegerfcar MT, Rezaian M, Nadim A, Anwar M, Afshar A. Animal toxoplasmosis in Iran. J Trop Med Hyg. 1983; 86(2): 73-6.
Hosseininejad, M. Hosseini, F. Seroprevalence of Neospora caninum and Toxoplasma gondii infec-tion in dogs from west and central parts of Iran using two indirect ELISA tests and assessment of associate risk factors. Iran J Vet Res. 2011; 121: 46
Hosseininejad M, Malmasi A, Hosseini F, Selk-Ghaffari M, Khorrami N, Mohebali M, Shojaee S, Mirani A, Azizzadeh M, Mirshokraei P, Aliari A. Seroprevalence of Toxoplasma gondii infection in dogs in Tehran, Iran. Iranian J Parasitol. 2011; l (6): 81-85.
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| Issue | Vol 8 No 3 (2013) | |
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