Morphometric Analysis of Larval Rostellar Hooks in Taenia multiceps of Sheep in Iran and Its Association with Mitochondrial Gene Variability



Background: The purposes of the present study were morphometric characteri-zation of rostellar hooks of Taenia multiceps and to investigate the association of hook length variation and the variability within two mitochondrial genes of sheep isolates of the parasite.

Methods: Up to 4500 sheep brains were examined for the presence of C. cerebralis. Biometric characters based on the larval rostellar hook size were measured for each individual isolate. Representative mitochondrial CO1 and 12S rRNA gene se-quences for each of the isolates were obtained from NCBI GenBank. Morphomet-ric and genetic data were analyzed using cluster analysis, Interclass Correlation Co-efficient (ICC) and random effects model.

Results: One hundred and fourteen sheep (2.5%) were found infected with the coenuri. The minimum and maximum number of scoleces per cyst was 40 and 550 respectively. Each scolex contained 22–27 hooks arranged in two rows of large and small hooks. The average total length of the large and small hooks was 158.9 and 112.1 μm, respectively. Using ICC, statistically significant clusters of different hook sizes were identified within the isolates. The length of the large and small hooks was significantly associated with the variability in mitochondrial 12S rRNA gene.

Conclusion: Taenia multiceps, is a relatively important zoonotic infection in Iranian sheep with the prevalence rate of 2.5%. Hook length analysis revealed statistically significant difference among individual isolates. Associations between the rostellar hook length and variability in the mitochondrial 12S rRNA was documented.

Abo-Shehada MN, Jebreen E, Arab B, Mukbel R, Torgerson PR. Prevalence of Taenia multiceps in sheep in northern Jordan. Pre Vet Med. 2002; 55(3):201-207.

Achenef M, Markos T, Feseha G, Hibret A, Tembely S. Coenurus cerebralis infection in Ethio-pian highland sheep: incidence and observa-tions on pathogenesis and clinical signs. Trop Anim Health Prod. 1999; 31(1):15-24.

Scott JC, Stefaniak J, Pawlowski ZS, McManus DP. Molecular genetic analysis of human cystic hydatid cases from Poland: identification of a new genotypic group (G9).Parasitology. 1997; 114:37-43.

Scala A, Varcasia A. Updates on morphobiol-ogy, epidemiology and molecular characteriza-tion of coenurosis in sheep. Parassitologia. 2006; 48(1-2):61-63.

Ing MB, Schantz PM, Turner JA. Human coe-nurosis in North America: case reports and re-view. Clin Infect Dis.1998; 27(3):519-523.

Tavassoli M, Malekifard F, Soleimanzadeh A, Tajik H. Prevalence of Coenurus cerebralis in sheep in Northwest of Iran. Vet Res Forum. 2011; 2(4):274-276.

Oryan, A, Moghaddar N , Gaur SNS. Metacestodes of sheep with special reference to their epidemiological status, pathogenesis and economic implications in Fars Province, Iran .Vet Parasitol.1994; 51(3-4):231-240.

Javadi A, Hashemzadeh H, .Shahbaz P. Study of Cerebral coenurosis rate in slaughtered sheep and goat brains in slaughterhouse in Tabriz. Iran Vet J. 2010; 6(1):59-63.

Yuossefi MR, Abohasani M, Hosseini SM, Omidzahir Sh. Prevalence rate of coenuros cere-bralis in Babol city slaughted sheeps. Iran Vet Res Bull. 2008; 6(2):97-99.

Arbabi M, Doroudgar A, Houshyar H, Moubedi I. A survey on carnivore's cestodes contamination in Kashan region. J Vet Res 2004; 59(3):289-293.

Dalimi A, Sattari A, Motamedi G. A study on intestinal helminthes of dogs, foxes and jackals in the western part of Iran. Vet Parasitol, 2006; 142(1):129-133.

Razmi GR, Sardari A, Kamrani AR. Prevalence of Echinococcus granulosus and other intestinal hel-minths of stray dogs in Mashhad area, Iran. Arch Razi Inst. 2006; 61(3):143-148.

Eslami A, Ranibar-Bahadori Sh, Meshki B, Dehghan M, Bokaie S. Helminth infections of stray dogs from Garmsar, Semnan Province, Central Iran. Iranian J Parasitol. 2010; 5(4):37.

Gicik Y, Kara M, Arslan MO. Prevalence of Coenurus cerebralis in sheep in Kars Province, Turkey. Bull Vet Inst Pulawy. 2007; .51:379.

Uslu U, Guclu F. Prevalence of Coenurus cere-bralis in sheep in Turkey. Medycyna Weterynar-yjna. 2007; 63(6):678-680.

Islam AW, Rahman MS. A report on incidence of gid of calves of Bangladesh. Indian J Anim Health.1997; 36:187–188.

Biyikoglu, G, Bagci O, Oncel T. A coenurosis outbreak of sheep in Istanbul, Turkey. Pendik Veteriner Mikrobiyoloji Dergisi.2001; 32 (1–2): 27–30.

Njau BC, Kasali OB, Scholtens, RG, Mesfin D. Review of sheep mortality in Ethiopian high-lands (ILCA Bulletin No. 31, Addis Ababa, Ethiopia) 1988:19–22 (cited by Achenef M, Markos T, Feescha G, Hibret A, Tembley S. Coenurus cerebralis infection in Ethiopian high-land sheep: incidence and observations on pathogenesis and clinical signs. Trop Anim Health Prod.1999; 15–24).

Mowlavi G, Salehi M, Eshraghian M, Rokni MB, Fasihi Harandi M, Mohajeran E, et al. Morphometric differentiation between camel and sheep strains of Echinococcus granulosus using computer image analysis system (CIAS). Asian Pac J Trop Med.2012; 5(1):58-61.

Harandi MF, Hobbs RP, Adams PJ, Mobedi I, Morgan-Ryan UM, Thompson RC. Molecular and morphological characterization of Echino-coccus granulosus of human and animal origin in Iran. Parasitology. 2002; 125(4):367-73.

Hobbs RP, Lymbery AJ, Thompson RC. Ros-tellar hook morphology of Echinococcus granulosus (Batsch, 1786) from natural and experimental Australian hosts, and its implications for strain recognition. Parasitology.1990; 101(2):273-281.

Edwards GT, Herbert IV. Some quantitative characters used in the identification of Taenia hydatigena, T. ovis, T. pisiformis and T. multiceps adult worms, and T. multiceps metacestodes. J Helminthol . 1981; 55(1):1-7.

Verster A. A taxonomic revision of the genus Taenia Linnaeus.1758.S.Str.Onderstepoort J Vet Res. 1969; 36(1):3-58.

Harandi MF, Hajialilo E, Shokouhi M. Larval Hook Length Measurement for Differentiating G1 and G6 Genotypes of Echinococcus granulosus Sensu Lato. Turkiye Parazitol Derg. 2012; 36(4):215-218.

Lymbery AJ. Combining data from morpho-logical traits and genetic markers to determine transmission cycles in the tape worm, Echinococ-cus granulosus. Parasitology. 1998; 117(2):185-192.

He SY, Nomura K, Whittam TS. Type III pro-tein secretion mechanism in mammalian and plant pathogens. Biochim Biophys Acta. 2004; 1694(1-3):181-206.

McCormack JE, Smith TB. Niche expansion leads to small-scale adaptive divergence along an elevation gradient in a medium-sized passer-ine bird. Proc Biol Sci. 2008; 275(1647):2155-2164.

Sharma. DK, Chauhan PPS. Coenurosis status in Afro-Asian region: a review. Small Rum Res. 2006; 64(3):197-202.

Abedl-Maogood SZ. Studies on metacestode of sheep with reference to serodiagnosis of Coenurus cerebralis.[PhD dissertation]. Faculty of Veterinary Medicine. Cairo University. 2005.

Scala A,Cancedda GM, Varcasia A,Liqios C, Garippa G, Genchi C. A survey of Taenia multi-ceps coenurosis in Sardinian sheep. Vet Parasitol. 2007; 143(3-4):294-298.

Razig SA, Magzoub M. Goat infected with Coenurus cerebralis: Clinical manifestations. Trop Anim Health Prod. 1973; 5(4):278-280.

Michal A, Regli F, Campiche R, Cavallo RJ, de Crousaz G, Oberson R, Rabinowicz T. Cerebral coenurosis. Report of a case with arteritis. J Neu-rol. 1977; 216(4):265-272.

Tirgari M, Howard BR, Boargob A. Clinical and radiographical diagnosis of coenurosis cere-bralis in sheep and its surgical treatment. Vet Rec.1987; 120(8A):173-178.

Varcasia A, Lightowers MW, Cattoli G, Can-cedda GM, Canu S, Garippa G, Scala A. Ge-netic variation within Taenia multiceps in Sardinia, Western Mediterranean (Italy). Parasitol Res. 2006; 99(5):622-626.

Swiderski Z. Electron microscopy and histo-chemistry of oncospheral hook formation by the cestode Catenotaenia pusilla .Int J Parasitol. 1973; 3(1):27-30.

Smith SA, Richards KS. Ultrastructure and microanalyses of the protoscolex hooks of Echinococcus granulosus .Parasitology. 1991; 103(02):267-274.

Hall MC. The adult Taenioid cestodes of dogs and cats, and of related carnivores in North America. J Mammal. 1919; 1(1):44-47.

Clapham PA. On identifying Multiceps spp. by measurement of the large hook. J Helminthol. 1942; 20(1-2): 31-40.

Elowni EE, Abu-samra MT, Ibrahim KEE, Hassan KI. The identity of coenuri from Suda-nese sheep and goats. Bull Anim Heal Afr. 1990; 38(4):345-350.

IssueVol 8 No 4 (2013) QRcode
Hook morphometry Iran Mitochondrial gene Sheep Taenia multiceps

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How to Cite
Rostami S, Beech RN, Salavati R, Baneshi MR, Kamyabi H, Harandi MF. Morphometric Analysis of Larval Rostellar Hooks in Taenia multiceps of Sheep in Iran and Its Association with Mitochondrial Gene Variability. Iran J Parasitol. 1;8(4):579-585.