The Infestation of Supplied Mincemeat to Sarcocystis Species and Their Polymorphism Using PCR-SSCP Method

Abstract

This study was carried on for determination of presence of Sarcocystis species in mincemeat in Tabriz (Iran) using PCR-SSCP method. Thirty samples of mixed ground meat was collected from butchers and DNA was extracted. The 18 sRNA gene fragment of S. cruzi and S. hominis species in the extracted DNA amplified by PCR using one pair specific primers , which resulted in 937 bp length fragments in parasite-positive samples. The results showed that 11 samples out of 30 (36%) were positive for S. cruzi and S. hominis species. The SSCP technique products of gene indicated 4 SSCP atterns (A, B, C and D) among study samples on PCR 18srRNA gene of S. cruzi and S. hominis. The results of this study showed that contamination with sarcocystis species in minced meat sold in Tabriz city in Iran is relatively high, which is of particular importance from a health point of view.This study was currently by SSCP teqnique on minced meat for the first time, based on the primers in the 18srRNA gene locus, there are polymorphisms in S.cruzi and S.hominis species.