Histopathologic Alterations between Echinococcus granulosus sensu stricto and E. canadensis Genotypes of Human Cystic Echinococcosis Cysts in Shiraz, Iran
-
Enayat Darabi
,
Seyed Mahmoud Sadjjadi
,
Tahereh Mohammadzadeh
,
Mehdi Karamian
,
Seyed Mohammad Owji
,
Bahareh Sedaghat
Abstract
Abstract
1. Romig T, Ebi D, Wassermann M. Taxonomy and molecular epidemiology of Echinococcus granulosus sensu lato. Vet Parasitol. 2015; 213(3–4):76–84.
2. Karamian M, Haghighi F, Hemmati M, et al. Heterogenity of Echinococcus canadensis genotype 6- the main causative agent of cystic echinococcosis in Birjand, Eastern Iran. Vet Parasitol. 2017; 245:78–85.
3. Jafari R, Sanei B, Baradaran A, et al. Genetic characterization of Echinococcus granulosus strains isolated from humans based on nad1 and cox1 gene analysis in Isfahan, central Iran. J Helminthol. 2018; 92(6):696–702.
4. Torgerson PR, Deplazes P. Echinococcosis: diagnosis and diagnostic interpretation in population studies. Trends Parasitol. 2009; 25(4):164–70.
5. Rokni MB. Echinococcosis/hydatidosis in Iran. Iran J Parasitol. 2009; 4(2): 1-16.
6. Abushhewa MH, Abushhiwa MH, Nolan MJ, et al. Genetic classification of Echinococcus granulosus cysts from humans, cattle and camels in Libya using mutation scanning-based analysis of mitochondrial loci. Mol Cell Probes. 2010; 24(6):346–51.
7. Pezeshki A, Akhlaghi L, Sharbatkhori M, et al. Genotyping of Echinococcus granulosus from domestic animals and humans from Ardabil Province, northwest Iran. J Helminthol. 2013; 87(4):387–91.
8. Sadjjadi SM, Mikaeili F, Karamian M, et al. Evidence that the Echinococcus granulosus G6 genotype has an affinity for the brain in humans. Int J Parasitol. 2013; 43(11):875–7.
9. Neysi H, Mohammadzadeh T, Sadjjadi SM. Genetic Diversity of Hepatic/Non-Hepatic Cystic Echinococcosis in Baqiyatallah Hospital, Tehran, Iran. Iran J Parasitol. 2020; 15(1):130–7.
10. Ebrahimipour M, Rezaeian S, Shirzadi MR, et al. Prevalence and risk factors associated with human cystic echinococcosis in Iran. J Parasit Dis. 2019; 43(3):385–92.
11. Rojas CAA, Romig T, Lightowlers MW. Echinococcus granulosus sensu lato genotypes infecting humans–review of current knowledge. Int J Parasitol. 2014; 44(1):9–18.
12. Sadjjadi SM, Ebrahimipour M, Sadjjadi FS. Comparison between Echinococcus granulosus sensu stricto (G1) and E. canadensis (G6) mitochondrial genes (cox1 and nad1) and their related protein models using experimental and bioinformatics analysis. Comput Biol Chem. 2019; 79:103–109.
13. McManus DP. Molecular discrimination of taeniid cestodes. Parasitol Int. 2006; 55 Suppl:S31-7.
14. McManus DP, Bowles J. Molecular genetic approaches to parasite identification: their value in diagnostic parasitology and systematics. Int J Parasitol. 1996; 26(7):687–704.
15. Sharbatkhori M, Mirhendi H, Jex AR, et al. Genetic categorization of Echinococcus granulosus from humans and herbivorous hosts in Iran using an integrated mutation scanning‐phylogenetic approach. Electrophoresis. 2009; 30(15):2648–55.
16. Gorgani-Firouzjaee T, Kalantrai N, Ghaffari S, et al. Genotype characterization of livestock and human cystic echinococcosis in Mazandaran province, Iran. J Helminthol. 2019; 93(2):255–9.
17. Siyadatpanah A, Daryani A, Sarvi S, et al. Phylogeography and genetic diversity of human hydatidosis in bordering the Caspian Sea, northern Iran by focusing on Echinococcus granulosus sensu stricto complex. Iran J Public Health. 2020; 49(9):1758-1768.
18. Rozas J, Sánchez-DelBarrio JC, Messeguer X, et al. DNA polymorphism analyses by the coalescent and other methods. Bioinformatics. 2003; 19(18):2496–7.
19. Shahbazi AE, Saidijam M, Maghsood AH, et al. Genotyping of fresh and parafinized human hydatid cysts using nad1 and cox1 genes in Hamadan Province, west of Iran. Iran J Parasitol. 2020; 15(2):259-265.
20. Al-Jawabreh A, Ereqat S, Dumaidi K, et al. The clinical burden of human cystic echinococcosis in Palestine, 2010-2015. PLoS Negl Trop Dis. 2017; 11(7):e0005717.
21. Kurt A, Avcioglu H, Guven E, et al. Molecular Characterization of Echinococcus multilocularis and Echinococcus granulosus from Cysts and Formalin-Fixed Paraffin-Embedded Tissue Samples of Human Isolates in Northeastern Turkey. Vector Borne Zoonotic Dis. 2020; 20(8):593–602.
22. Rostami S, Torbaghan SS, Dabiri S, et al. Genetic characterization of Echinococcus granulosus from a large number of formalin-fixed, paraffin-embedded tissue samples of human isolates in Iran. Am J Trop Med Hyg. 2015; 92(3):588-94.
23. Kheirandish F, Mahmoudvand H, Ahmadinejad M, et al. Genetic cha-racterization of human-derived hydatid cysts of Echinococcus granulosus in Lorestan Province, Western Iran. Trop Biomed.2017; 34(4): 863-869.
24. Moradi M, Meamar AR, Akhlaghi L, et al. Detection and genetic characterization of Echinococcus granulosus mitochondrial DNA in serum and formalin-fixed paraffin embedded cyst tissue samples of cystic echinococcosis patients. PLoS One. 2019; 14(10):e0224501.
25. Koohestan MG, Saberi R, Daryani A, et al. Identification and genotyping of Echinococcus granulosus from human clinical samples in Guilan province, north of Iran. Parasite Epidemiol Control. 2024; 25:e00353.
26. Elyasi H, Farbodnia T, Javaheri E. Genetic Characteristics of Echinococcus granulosus from Fixed Paraffin-Embedded Tissue Samples in Human Isolates Based on the High-Resolution Melting Point Analysis in Sabzevar, Northeast Iran. Iran J Parasitol. 2024; 19(3):305-313.
27. Tamura K, Peterson D, Peterson N, et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol. 2011; 28(10):2731–9.
28. Leigh JW, Bryant D. POPART: full-feature software for haplotype network construction. Methods Ecol Evol. 2015; 6(9):1110–6.
29. Yang YR, Craig PS, Vuitton DA, et al. Serological prevalence of echinococcosis and risk factors for infection among children in rural communities of southern Ningxia, China. Trop Med Int Health. 2008; 13(8):1086–94.
30. Shahnazi M, Hejazi H, Salehi M, et al. Molecular characterization of human and animal Echinococcus granulosus isolates in Isfahan, Iran. Acta Trop. 2011; 117(1):47–50.
31. Simsek S, Kaplan M, Ozercan IH. A comprehensive molecular survey of Echinococcus granulosus in formalin-fixed paraffin-embedded tissues in human isolates in Turkey. Parasitol Res. 2011; 109:411–6.
32. Sharbatkhori M, Tanzifi A, Rostami S, et al. Echinococcus granulosus sensu lato genotypes in domestic livestock and humans in Golestan province, Iran. Rev Inst Med Trop Sao Paulo. 2016; 58:38.
33. Spotin A, Mahami-Oskouei M, Harandi MF, et al. Genetic variability of Echinococcus granulosus complex in various geographical populations of Iran inferred by mitochondrial DNA sequences. Acta Trop. 2017; 165:10–16.
34. Hajialilo E, Harandi MF, Sharbatkhori M, et al. Genetic characterization of Echinococcus granulosus in camels, cattle and sheep from the south-east of Iran indicates the presence of the G3 genotype. J Helminthol. 2012; 86(3):263–70.
35. Sharbatkhori M, Mirhendi H, Harandi MF, et al. Echinococcus granulosus genotypes in livestock of Iran indicating high frequency of G1 genotype in camels. Exp Parasitol. 2010; 124(4):373–9.
36. Mohaghegh MA, Yousofi-Darani H, Jafarian AH, et al. Isolated Human and Livestock Echinococcus granulosus Genotypes Using Real-Time PCR of cox1 Gene in Northeast Iran. Acta Parasitol. 2019; 64(3):679–85.
37. Shirmen O, Batchuluun B, Lkhamjav A, et al. Cerebral cystic echinococcosis in Mongolian children caused by Echinococcus canadensis. Parasitol Int. 2018; 67(5):584–6.
38. Kargar S, Dalimi A, Eslami G, et al. Cystic Echinococcosis in Central Iran: G1 and G6 Genotypes in Patients. Surg Infect (Larchmt). 2022; 23(5):451–7.
39. Mirahmadi H, Behravan M, Raz A, et al. Genotyping of the Echinococcus granulosus in Paraffin-Embedded Human Tissue Samples from Iran. Acta Parasitol. 2021; 66(2):535–42.
40. Shafiei R, Ghatee MA, Jafarzadeh F, et al. Genotyping and phylogenetic analysis of unusually located hydatid cysts isolated from humans in north-east Iran. J Helminthol. 2019; 94:e64.
41. Parsa F, Harandi MF, Rostami S, et al. Genotyping Echinococcus granulosus from dogs from Western Iran. Exp Parasitol. 2012; 132(2):308–12.
42. Guarnera EA, Parra A, Kamenetzky L, et al. Cystic echinococcosis in Argentina: evolution of metacestode and clinical expression in various Echinococcus granulosus strains. Acta Trop. 2004; 92(2):153–9.
43. Yanagida T, Mohammadzadeh T, Kamhawi S, et al. Genetic polymorphisms of Echinococcus granulosus sensu stricto in the Middle East. Parasitol Int. 2012; 61(4):599–603.
44. Alvarez Rojas CA, Kronenberg PA, Aitbaev S, et al. Genetic diversity of Echinococcus multilocularis and Echinococcus granulosus sensu lato in Kyrgyzstan: The A2 haplotype of E. multilocularis is the predominant variant infecting humans. PLoS Negl Trop Dis. 2020; 14(5):e0008242.
45. Hamedani NA, Pirestani M, Dalimi A. Clinical and molecular features of human cystic echinococcosis in Tehran, Iran, 2011-2019. Asian Pac J Trop Med. 2022; 15(12):558-67.
2. Karamian M, Haghighi F, Hemmati M, et al. Heterogenity of Echinococcus canadensis genotype 6- the main causative agent of cystic echinococcosis in Birjand, Eastern Iran. Vet Parasitol. 2017; 245:78–85.
3. Jafari R, Sanei B, Baradaran A, et al. Genetic characterization of Echinococcus granulosus strains isolated from humans based on nad1 and cox1 gene analysis in Isfahan, central Iran. J Helminthol. 2018; 92(6):696–702.
4. Torgerson PR, Deplazes P. Echinococcosis: diagnosis and diagnostic interpretation in population studies. Trends Parasitol. 2009; 25(4):164–70.
5. Rokni MB. Echinococcosis/hydatidosis in Iran. Iran J Parasitol. 2009; 4(2): 1-16.
6. Abushhewa MH, Abushhiwa MH, Nolan MJ, et al. Genetic classification of Echinococcus granulosus cysts from humans, cattle and camels in Libya using mutation scanning-based analysis of mitochondrial loci. Mol Cell Probes. 2010; 24(6):346–51.
7. Pezeshki A, Akhlaghi L, Sharbatkhori M, et al. Genotyping of Echinococcus granulosus from domestic animals and humans from Ardabil Province, northwest Iran. J Helminthol. 2013; 87(4):387–91.
8. Sadjjadi SM, Mikaeili F, Karamian M, et al. Evidence that the Echinococcus granulosus G6 genotype has an affinity for the brain in humans. Int J Parasitol. 2013; 43(11):875–7.
9. Neysi H, Mohammadzadeh T, Sadjjadi SM. Genetic Diversity of Hepatic/Non-Hepatic Cystic Echinococcosis in Baqiyatallah Hospital, Tehran, Iran. Iran J Parasitol. 2020; 15(1):130–7.
10. Ebrahimipour M, Rezaeian S, Shirzadi MR, et al. Prevalence and risk factors associated with human cystic echinococcosis in Iran. J Parasit Dis. 2019; 43(3):385–92.
11. Rojas CAA, Romig T, Lightowlers MW. Echinococcus granulosus sensu lato genotypes infecting humans–review of current knowledge. Int J Parasitol. 2014; 44(1):9–18.
12. Sadjjadi SM, Ebrahimipour M, Sadjjadi FS. Comparison between Echinococcus granulosus sensu stricto (G1) and E. canadensis (G6) mitochondrial genes (cox1 and nad1) and their related protein models using experimental and bioinformatics analysis. Comput Biol Chem. 2019; 79:103–109.
13. McManus DP. Molecular discrimination of taeniid cestodes. Parasitol Int. 2006; 55 Suppl:S31-7.
14. McManus DP, Bowles J. Molecular genetic approaches to parasite identification: their value in diagnostic parasitology and systematics. Int J Parasitol. 1996; 26(7):687–704.
15. Sharbatkhori M, Mirhendi H, Jex AR, et al. Genetic categorization of Echinococcus granulosus from humans and herbivorous hosts in Iran using an integrated mutation scanning‐phylogenetic approach. Electrophoresis. 2009; 30(15):2648–55.
16. Gorgani-Firouzjaee T, Kalantrai N, Ghaffari S, et al. Genotype characterization of livestock and human cystic echinococcosis in Mazandaran province, Iran. J Helminthol. 2019; 93(2):255–9.
17. Siyadatpanah A, Daryani A, Sarvi S, et al. Phylogeography and genetic diversity of human hydatidosis in bordering the Caspian Sea, northern Iran by focusing on Echinococcus granulosus sensu stricto complex. Iran J Public Health. 2020; 49(9):1758-1768.
18. Rozas J, Sánchez-DelBarrio JC, Messeguer X, et al. DNA polymorphism analyses by the coalescent and other methods. Bioinformatics. 2003; 19(18):2496–7.
19. Shahbazi AE, Saidijam M, Maghsood AH, et al. Genotyping of fresh and parafinized human hydatid cysts using nad1 and cox1 genes in Hamadan Province, west of Iran. Iran J Parasitol. 2020; 15(2):259-265.
20. Al-Jawabreh A, Ereqat S, Dumaidi K, et al. The clinical burden of human cystic echinococcosis in Palestine, 2010-2015. PLoS Negl Trop Dis. 2017; 11(7):e0005717.
21. Kurt A, Avcioglu H, Guven E, et al. Molecular Characterization of Echinococcus multilocularis and Echinococcus granulosus from Cysts and Formalin-Fixed Paraffin-Embedded Tissue Samples of Human Isolates in Northeastern Turkey. Vector Borne Zoonotic Dis. 2020; 20(8):593–602.
22. Rostami S, Torbaghan SS, Dabiri S, et al. Genetic characterization of Echinococcus granulosus from a large number of formalin-fixed, paraffin-embedded tissue samples of human isolates in Iran. Am J Trop Med Hyg. 2015; 92(3):588-94.
23. Kheirandish F, Mahmoudvand H, Ahmadinejad M, et al. Genetic cha-racterization of human-derived hydatid cysts of Echinococcus granulosus in Lorestan Province, Western Iran. Trop Biomed.2017; 34(4): 863-869.
24. Moradi M, Meamar AR, Akhlaghi L, et al. Detection and genetic characterization of Echinococcus granulosus mitochondrial DNA in serum and formalin-fixed paraffin embedded cyst tissue samples of cystic echinococcosis patients. PLoS One. 2019; 14(10):e0224501.
25. Koohestan MG, Saberi R, Daryani A, et al. Identification and genotyping of Echinococcus granulosus from human clinical samples in Guilan province, north of Iran. Parasite Epidemiol Control. 2024; 25:e00353.
26. Elyasi H, Farbodnia T, Javaheri E. Genetic Characteristics of Echinococcus granulosus from Fixed Paraffin-Embedded Tissue Samples in Human Isolates Based on the High-Resolution Melting Point Analysis in Sabzevar, Northeast Iran. Iran J Parasitol. 2024; 19(3):305-313.
27. Tamura K, Peterson D, Peterson N, et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol. 2011; 28(10):2731–9.
28. Leigh JW, Bryant D. POPART: full-feature software for haplotype network construction. Methods Ecol Evol. 2015; 6(9):1110–6.
29. Yang YR, Craig PS, Vuitton DA, et al. Serological prevalence of echinococcosis and risk factors for infection among children in rural communities of southern Ningxia, China. Trop Med Int Health. 2008; 13(8):1086–94.
30. Shahnazi M, Hejazi H, Salehi M, et al. Molecular characterization of human and animal Echinococcus granulosus isolates in Isfahan, Iran. Acta Trop. 2011; 117(1):47–50.
31. Simsek S, Kaplan M, Ozercan IH. A comprehensive molecular survey of Echinococcus granulosus in formalin-fixed paraffin-embedded tissues in human isolates in Turkey. Parasitol Res. 2011; 109:411–6.
32. Sharbatkhori M, Tanzifi A, Rostami S, et al. Echinococcus granulosus sensu lato genotypes in domestic livestock and humans in Golestan province, Iran. Rev Inst Med Trop Sao Paulo. 2016; 58:38.
33. Spotin A, Mahami-Oskouei M, Harandi MF, et al. Genetic variability of Echinococcus granulosus complex in various geographical populations of Iran inferred by mitochondrial DNA sequences. Acta Trop. 2017; 165:10–16.
34. Hajialilo E, Harandi MF, Sharbatkhori M, et al. Genetic characterization of Echinococcus granulosus in camels, cattle and sheep from the south-east of Iran indicates the presence of the G3 genotype. J Helminthol. 2012; 86(3):263–70.
35. Sharbatkhori M, Mirhendi H, Harandi MF, et al. Echinococcus granulosus genotypes in livestock of Iran indicating high frequency of G1 genotype in camels. Exp Parasitol. 2010; 124(4):373–9.
36. Mohaghegh MA, Yousofi-Darani H, Jafarian AH, et al. Isolated Human and Livestock Echinococcus granulosus Genotypes Using Real-Time PCR of cox1 Gene in Northeast Iran. Acta Parasitol. 2019; 64(3):679–85.
37. Shirmen O, Batchuluun B, Lkhamjav A, et al. Cerebral cystic echinococcosis in Mongolian children caused by Echinococcus canadensis. Parasitol Int. 2018; 67(5):584–6.
38. Kargar S, Dalimi A, Eslami G, et al. Cystic Echinococcosis in Central Iran: G1 and G6 Genotypes in Patients. Surg Infect (Larchmt). 2022; 23(5):451–7.
39. Mirahmadi H, Behravan M, Raz A, et al. Genotyping of the Echinococcus granulosus in Paraffin-Embedded Human Tissue Samples from Iran. Acta Parasitol. 2021; 66(2):535–42.
40. Shafiei R, Ghatee MA, Jafarzadeh F, et al. Genotyping and phylogenetic analysis of unusually located hydatid cysts isolated from humans in north-east Iran. J Helminthol. 2019; 94:e64.
41. Parsa F, Harandi MF, Rostami S, et al. Genotyping Echinococcus granulosus from dogs from Western Iran. Exp Parasitol. 2012; 132(2):308–12.
42. Guarnera EA, Parra A, Kamenetzky L, et al. Cystic echinococcosis in Argentina: evolution of metacestode and clinical expression in various Echinococcus granulosus strains. Acta Trop. 2004; 92(2):153–9.
43. Yanagida T, Mohammadzadeh T, Kamhawi S, et al. Genetic polymorphisms of Echinococcus granulosus sensu stricto in the Middle East. Parasitol Int. 2012; 61(4):599–603.
44. Alvarez Rojas CA, Kronenberg PA, Aitbaev S, et al. Genetic diversity of Echinococcus multilocularis and Echinococcus granulosus sensu lato in Kyrgyzstan: The A2 haplotype of E. multilocularis is the predominant variant infecting humans. PLoS Negl Trop Dis. 2020; 14(5):e0008242.
45. Hamedani NA, Pirestani M, Dalimi A. Clinical and molecular features of human cystic echinococcosis in Tehran, Iran, 2011-2019. Asian Pac J Trop Med. 2022; 15(12):558-67.
| Files | ||
| Issue | Vol 20 No 1 (2025) | |
| Section | Original Article(s) | |
| Keywords | ||
| Cystic echinococcosis Iran Parasitology Histopathology Genotyping Haplotyping | ||
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How to Cite
1.
Darabi E, Sadjjadi SM, Mohammadzadeh T, Karamian M, Owji SM, Sedaghat B. Histopathologic Alterations between Echinococcus granulosus sensu stricto and E. canadensis Genotypes of Human Cystic Echinococcosis Cysts in Shiraz, Iran. Iran J Parasitol. 2025;20(1):21-31.
