Articles

Detection of Toxoplasma gondii Antigens in Sera and Urine of Experimentally Infected Mice by Capture ELISA

Abstract

Background: Toxoplasma gondii is an apicomplexan parasite which infects a broad range of hosts. The classical diagnosis of toxoplasmosis relies on serological methods. Detection of parasite or its components could be useful tool for early diag­nosis of the infection.

Methods: Fifty mice were infected by the intraperitoneal route with 5000 tachyzoites of T. gondii RH strain. Five of them sacrificed every day from day 1 up to day 7 post infection. Sera and urine of mice were tested by capture ELISA.

Results: T.gondii antigens were detected from 3rd and 2nd day in serum and urine, respectively, after infection until their death on day 7.

Conclusion: Antigenemia detection of antigens of parasite was possible in a short period of acute infection with T. gondii by capture ELISA.

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IssueVol 3 No 1 (2008) QRcode
SectionArticles
Keywords
Capture ELISA
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Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
How to Cite
1.
F Foroghi parvar, H Keshavarz, S Shojaee. Detection of Toxoplasma gondii Antigens in Sera and Urine of Experimentally Infected Mice by Capture ELISA. Iran J Parasitol. 1;3(1):1-5.