Designing a Multiple-Epitope Vaccine Candidate against Leishmania major and Leishmania infantum for Monocyte-Derived Exosome Preparation
-
Ali Moazezi Ghavihelm
,
Sedigheh Nabian
,
Shahram Jamshidi
,
Mohammad Taheri
,
Minoo Soltani
,
Ramin Mazaheri Nezhad Fard
,
Ali Akbari Pazoki
Abstract
Background: Leishmania is a vector-borne protozoon, which causes visceral, cutaneous and mucocutaneous leishmaniosis in human and animals. Monocyte-derived exosome vaccines can be used as prophylaxis and immunotherapy strategies. The aim of this study was to design a multiple-epitope candidate vaccine using leishmaniolysin (GP63) and rK39 proteins against Leishmania major and L. infantum for monocyte-derived exosome preparation.
Methods: This study was carried out in Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran, 2023–2024. Effective immunodominant epitopes were selected from two antigenic proteins of GP63 and rK39 using various immunoinformatics and bioinformatics approaches. Vibrio cholerae β-subunit was used as an adjuvant to stimulate immune responses. Then, appropriate linkers were selected for the fusion of epitopes. The 3D model of candidate vaccine was predicted and validated.
Results: This designed candidate vaccine could effectively be used as a prophylaxis strategy against leishmaniosis.
Conclusion: A candidate vaccine was designed using bioinformatic and immunoinformatic studies with virtual acceptable quality; however, effectiveness of this vaccine should be verified through further in-vitro and in-vivo studies.
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13. da Silva L F, Fajardo E, Chang K, et al. Leishmania Exosomes/extracellular vesicles containing GP63 are essential for enhance cutaneous leishmaniasis development upon co-inoculation of Leishmania amazonensis and its exosomes. Front Cell Infect Microbiol. 2022; 11:709258.
14. Beauvillain C, Ruiz S, Guiton R, et al. A vaccine based on exosomes secreted by a dendritic cell line confers protection against T. gondii infection in syngeneic and allogeneic mice. Microbes Infect. 2007; 9(14-15): 1614-22.
15. Hao S, Bai O, Fang Li, et al. Mature dendritic cells pulsed with exosomes stimulate efficient cytotoxic T-lymphocyte responses and antitumour immunity. Immunology. 2007; 120(1): 90-102.
16. Misra N, Panda PK, Shah K, et al. Population coverage analysis of T-cell epitopes of Neisseria meningitidis serogroup b from iron acquisition proteins for vaccine design. Bioinformation. 2011; 6(7):255–61.
17. Longmate J, York J, La Rosa C, et al. Population coverage by HLA class-I restricted cytotoxic T-lymphocyte epitopes. Immunogenetics. 2001; 52(3-4): 165–73.
18. Ishwarlall Z, Adeleke T , Maharaj L, et al. Identification of potential candidate vaccines against Mycobacterium ulcerans based on the major facilitator superfamily transporter protein, Front Immunol. 2022; 13:1023558.
19. Naz A, Shahid F , Tahir Butt T, et al. Designing multi-epitope vaccines to combat emerging Coronavirus disease 2019 (COVID-19) by employing immuno-informatics approach. Front Immunol. 2020; 11: 1663.
20. Doolan D L, Southwood S, Chesnut R , et al. HLA-DR-promiscuous T cell epitopes from Plasmodium falciparum pre-erythrocytic-stage antigens restricted by multiple HLA class II alleles. J Immunol. 2000; 165(2):1123-37.
21. Andre F, Chaput N, Schartz NE, et al. Exosomes as potent cell-free peptide-based vaccine. I. Dendritic cell-derived exosomes transfer functional MHC class I/peptide complexes to dendritic cells. J Immunol. 2004; 172(4): 2126–2136.
22. Hiltbrunner S, Larssen P, Eldh M, et al. Exosomal cancer immunotherapy is independent of MHC molecules on exosomes. Oncotarget. 2016; 7(25): 38707-38717.
2. Rezaei Z , Van Reet N, Pouladfar G, Vera Kuhne, Ramezani A, Sarkari B, Pourabbas B, Büscher P. Expression of a rK39 homologue from an Iranian Leishmania infantum isolate in Leishmania tarentolae for serodiagnosis of visceral leishmaniasis. Parasit Vectors. 2019; 12:593.
3. de Oliveira LMF, Morale MG, Chaves AAM, et al. Design, immune responses and anti-tumor potential of an HPV16 E6E7 multi-epitope vaccine. PLoS One. 2015; 10(9):e0139686.
4. Smith V L, Cheng Y, Bryant B R, Schorey J S. Exosomes function in antigen presentation during an in vivo Mycobacterium tuberculosis infection. Sci Rep. 2017|; 7:43578.
5. da Silva L F, Fajardo E, Chang K, Pauline Clément, Olivier M. Leishmania Exosomes/extracellular vesicles containing GP63 are essential for enhance cutaneous leishmaniasis development upon co-inoculation of Leishmania amazonensis and its exosomes. Front Cell Infect Microbiol. 2022; 11:709258.
6. Taïeb J, Chaput N, Zitvogel L. Dendritic cell-derived exosomes as cell-free peptide-based vaccines. Crit Rev Immunol. 2005; 25(3): 215-23.
7. Nikfarjam S, Rezaie J , Kashanchi F , Jafari R. Dexosomes as a cell-free vaccine for cancer immunotherapy. J Exp Clin Cancer Res. 2020; 39: 258.
8. Samuel M, Gabrielsson S. Personalized medicine and back–allogeneic exosomes for cancer immunotherapy. J Intern Med. 2021; 289(2):138-146.
9. Moyle PM, Toth I. "Modern subunit vaccines: development, components and research opportunities". ChemMedChem. 2013; 8(3): 360-376.
10. Abedini F, Rahmanian N, Heidari Z, Feizi A, Sherkat R, Rezaei M. Diversity of HLA class I and class II alleles in Iran populations: Systematic review and Meta-Analaysis. Transpl Immunol. 2021; 69: 101472.
11. Dariushnejad H, Ghorbanzadeh V, Akbari S, Hashemzadeh P. Design of a Novel Recombinant Multi-Epitope Vaccine against Triple-Negative Breast Cancer. Iran Biomed J. 2022; 26(2): 160-74.
12. Minhas V, Shrestha A, Wadhwa N, et al. Novel sperm and gonadotropin-releasing hormone-based recombinant fusion protein: Achievement of 100% contraceptive efficacy by co-immunization of male and female mice. Mol Reprod Dev. 2016; 83(12): 1048-1059.
13. da Silva L F, Fajardo E, Chang K, et al. Leishmania Exosomes/extracellular vesicles containing GP63 are essential for enhance cutaneous leishmaniasis development upon co-inoculation of Leishmania amazonensis and its exosomes. Front Cell Infect Microbiol. 2022; 11:709258.
14. Beauvillain C, Ruiz S, Guiton R, et al. A vaccine based on exosomes secreted by a dendritic cell line confers protection against T. gondii infection in syngeneic and allogeneic mice. Microbes Infect. 2007; 9(14-15): 1614-22.
15. Hao S, Bai O, Fang Li, et al. Mature dendritic cells pulsed with exosomes stimulate efficient cytotoxic T-lymphocyte responses and antitumour immunity. Immunology. 2007; 120(1): 90-102.
16. Misra N, Panda PK, Shah K, et al. Population coverage analysis of T-cell epitopes of Neisseria meningitidis serogroup b from iron acquisition proteins for vaccine design. Bioinformation. 2011; 6(7):255–61.
17. Longmate J, York J, La Rosa C, et al. Population coverage by HLA class-I restricted cytotoxic T-lymphocyte epitopes. Immunogenetics. 2001; 52(3-4): 165–73.
18. Ishwarlall Z, Adeleke T , Maharaj L, et al. Identification of potential candidate vaccines against Mycobacterium ulcerans based on the major facilitator superfamily transporter protein, Front Immunol. 2022; 13:1023558.
19. Naz A, Shahid F , Tahir Butt T, et al. Designing multi-epitope vaccines to combat emerging Coronavirus disease 2019 (COVID-19) by employing immuno-informatics approach. Front Immunol. 2020; 11: 1663.
20. Doolan D L, Southwood S, Chesnut R , et al. HLA-DR-promiscuous T cell epitopes from Plasmodium falciparum pre-erythrocytic-stage antigens restricted by multiple HLA class II alleles. J Immunol. 2000; 165(2):1123-37.
21. Andre F, Chaput N, Schartz NE, et al. Exosomes as potent cell-free peptide-based vaccine. I. Dendritic cell-derived exosomes transfer functional MHC class I/peptide complexes to dendritic cells. J Immunol. 2004; 172(4): 2126–2136.
22. Hiltbrunner S, Larssen P, Eldh M, et al. Exosomal cancer immunotherapy is independent of MHC molecules on exosomes. Oncotarget. 2016; 7(25): 38707-38717.
| Files | ||
| Issue | Vol 19 No 2 (2024) | |
| Section | Original Article(s) | |
| DOI | https://doi.org/10.18502/ijpa.v19i2.15851 | |
| Keywords | ||
| Leishmania Vaccine Epitopes Exosome | ||
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
How to Cite
1.
Moazezi Ghavihelm A, Nabian S, Jamshidi S, Taheri M, Soltani M, Mazaheri Nezhad Fard R, Akbari Pazoki A. Designing a Multiple-Epitope Vaccine Candidate against Leishmania major and Leishmania infantum for Monocyte-Derived Exosome Preparation. Iran J Parasitol. 2024;19(2):153-161.
