Morphological and Genetic Characteristics of the Liver Hydatid Cyst of a Donkey with Iran Origin

  • Ali Eslami Dept. of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
  • Parviz Shayan Mail Dept. of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
  • Said Bokaei Dept. of Epidemiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
COX I, DNA, Donkey, Echinococcus granulosus, Iran, Morphometric


Background: No data is available on morphology and genetic characteristics of Echinococcus granulosus derived from donkeys of Iran, despite of its existence in donkeys.In the present study morphometric variations of the rostellar hooks of protoscoleces and genotype characteristics of hydatid cyst of donkey from Iran were determined.

Methods: Protoscoleces prepared from hydatid cyst of donkey of Iran were morphometric and genetic analyzed. The genetic analysis was done using Cox 1 gene by comparative sequence analysis.

Results: Our morphometric results showed that donkey of Iran shares 6 out of 7 determined parameters with donkeys of Jordan and 4 out of 7 with 4 available data with Switzerland donkeys. Morphological similarities and dissimilarities were observed with sheep-dog (G1) and camel-dog strains (G6) of Iran. The nucleotide sequence alignment showed that the partial sequence of Cox 1 from donkey had 91% homology with query coverage of 99% to the corresponding sequence of E.equinus, 90% homology to the E. felidis, 90% homology to E. ortleppi, 89% homology to the E. shiquinus, 89% homology to the E. vogeli, 89% homology to the E. oligarthrus, 88% homology to the E. canadensis and 83% homology to the Taenia solium.Additionally, the amino acid sequence of this gene has also some differences between this strain and all known strains of E. granulosus even with E. equinus (G4).

Conclusion: Despite of common morphological characteristics of Iranian donkey hydatid cyst with those of donkeys of other parts of the world, genetically it has its.

own entity.


Eslami A. Veterinary Helminthology, University of Tehran Press, Iran, 2005.

Eslami A, Rahbari S, Meydani M. Cestodes and trematodes of wild sheep,Ovis ammon orientalis and goitered gazelle,Gazella subgutturosa in Iran.Vet Parasitol. 1981; 8:99-101.

Rokni,MB. Echinococcosis/hydatidosis in Iran. Iran J Parasitol. 2009; 4(2):1-16.

Eslami A, Nadealian MG. Cestode and trematode infections of equines in Iran. J Vet Fac Univ Tehran. 1987; 42:33-39.

Eslami A, Farsad Hamdi S. Helminth parasites of wild boar, sus scrofa in Iran. J wildl Dis. 1992; 28:316-318.

Eslami A, Hosseini SH. Echinococcus granulosus in infection of farm dogs of Iran. Parasitol Res. 1998; 84:205-207.

Abdi J, Kazemi B, Haniloo A, Mohebali M, Mahmoudi M, Rezaei S, Bandehpour M, Maghen L, Rokni MB. Serological Evaluation of EgAgB16 kDa, a Recombinant Antigen from Echinococcus granulosus for Diagnosis of Human Hydatidosis. Iran J Parasitol. 2010; 5(3): 1-10.

Taghipour N, Bandepour M, Pazoki R, Haghighi A, Nazari Pouya MR, Kazemi B. Subcloning and Expression of Recombinant Echinococcus granulosus Antigen B, in Pqe-30 Expression Vector. Iran J Parasitol. 2009; 4(4): 1-9.

Rahimi HR, Kia EB, Mirhendi SH, Talebi A, Fasihi Harandi M, Jalali-zand N, Rokni MB. A New Primer Pair in ITS1 Region for Molecular Studies on Echinococcus granulosus. Iran J Public Health. 2007; 36(1): 45-49.

Meshgi B, Eslami A, Bahonar AR, Khrrazian-Moghadam M, Gerami-Sadeghian A. Prevalence of parasitic infections in the red fox (Vulpes vulpes) and golden jackal (Canis aureus) in Iran. Iranian J Vet Res Shiraz Univ. 2009; 16(4): 387-391.

Thompson R, McManus D. Toward a taxonomic revision of the genus Echinococcus. Trends Parasitol. 2002; 18: 452-457.

William RJ, Sweatman GK. On the transmission, biology, morphology of Echinococcus granulosus, a new subspecies of hydatid tapeworm in horse in Great Britain. Parasitology. 1963; 53: 91-407.

Cuesta- Bandera C, McManus DP, Rishi AK. Characterization of Echinococcus granulosus of Spanish origin by DNA restriction endonuclease analysis and Southern blot hybridization. Int J Parasitol. 1988; 18(1):137-141.

Kumaratilake LM, Thompson RCA, Eckert J. Echinococcus granulosus of equine origin from different countries possess uniform morphological chrachteristics. Int J Parasitol. 1986; 16(5): 529-540.

Hoberg EP, Miller S, Browm MA. Echinococcus granulosus (Taniidae)and authocthonous echinococcosis in the North American horse. J Parasitol. 1994; 80(1): 141-144.

Daily MD, Sweatman GK. Taxonomy of Echinococcus granulosus in the donkey and dromedary in Lebanon and Syria. Ann Trop Med Parasitol. 1965; 59: 463-477.

Mukbel RM, Torgerson, PR, Abo-Shehada, MN. Prevalence of hydatidosis among donkeys in northern Jordan. Vet Parasitol. 2000; 88:35-42.

Ihasan M, Abdel-Hafez SK, Fadwa M, Al-Yaman, M. Morphological variation of Echinococcus granulosus protoscoleces from hydatid cyst of human and various domestic animals in Jordan. Int J Parasitol. 1988; 18(8): 1111-1114.

Azlaf A, Dakkak A. Epidemiological study of the cystic echinococcosis in Morocco. Vet Parasitol. 2006; 137:83-89.

Varcasia A, Garippa G, ,Pipia AP, Scala A, Brianti E, Giannetto S, Batelli G, Poglayen G, Micagni G. Cystic echinococcosis in equids in Italy. Parasitol Res. 2008; 102:815-818.

Zhang L, Eslami A, Hosseini SH, McManus DP. Indication of the presence of two distinct strains of Echinicoccus granulosus in Iran by mitochondrial DNA markers. Am J Trop Med Hyg. 1998; 59: 171-174.

Polydorou K. Animal health and economy. Case study:Echinococcosis with a reference to Cyprus. Bull Int Epiz. 1981; 93: 981-992.

Suothwell T. Experimental infection of the cat and the fox with adult Echinococcus granulosus. Ann Trop Med Parasit. 1927; 21:151.

Gallo C. L,echinococcosi et la distomatosidegli equine in Sicilia. Att Soc Ital Sci Vet. 1955; 9: 689-691.

Zhdanova MG. Hydatidosis in odd toed ungulates in the Uzbec S.S.R. (In Russian)Nauchnye Trudy Samarkandskogo Sel'skokhozyaistvennogo Institute im V.V. Kuibysheva. 1972; 24:151-154.

Pandey VS. Hydatidosis in donkeys in Morocco. Ann Trop Med Parasitol. 1980; 74(5):519-521.

Corsalini T. Frequenza dell' idatidosi nei soggetti delle diversespecie animalimacellati a Bari. Veter Ital. 1959; 10: 644-647.

Hosseini, SH, Eslami A. Morphological and developmental characteristics of Echinococcus granulosus derived from sheep, cattle and camel in Iran. J Helminthol. 1998; 72:337-341.

Lavikainen A, Lehtinen MJ, Meri T, Hirvela- Koski V, Meri S. Molecular genetic characterization of the Fennoscandian cervid strain a new genotype group (G10) of Echinococcus granulosus. Parasitology. 2003; 124: 207-215.

Nako M, McManus DP, Schantz PM, Craig PS, Ito A. A molecular phylogeny of the genus Echinococcus inferred from complete mithocondrial genomes. Parasitology. 2007: 134: 1-1.

How to Cite
Eslami A, Shayan P, Bokaei S. Morphological and Genetic Characteristics of the Liver Hydatid Cyst of a Donkey with Iran Origin. Iran J Parasitol. 9(3):302-310.