Immunological Analysis of Aerobic Bioreactor Bovine Theileriosis Vaccine

  • Gholamreza Habibi Mail Department of Parasite Vaccine Research and Production, Razi Vaccine and Serum Research Institute, Karaj, Iran.
  • Kasra Esmaeil-Nia Department of Parasite Vaccine Research and Production, Razi Vaccine and Serum Research Institute, Karaj, Iran.
  • Hasan Izadi Department of Foot and Mouth Disease Vaccine production, Razi Vaccine and Serum Research Institute, Karaj, Iran.
  • Orang Ataie Amarloie Department of Clinical Sciences, Faculty of Veterinary Sciences, Islamic Azad University, Karaj, Iran.
  • Asghar Afshari Department of Parasite Vaccine Research and Production, Razi Vaccine and Serum Research Institute, Karaj, Iran.
  • Nadia Bordbar Department of Quality Control of Biological Products, Razi Vaccine and Serum Research Institute, Karaj, Iran.
Aerobic Bioreactor, DTH, TNF-α, Theileriosis Vaccine


Background: In this study, the pilot production of aerobic bioreactor tropical theileriosis vaccine was optimized with the aim of immunological assays for further mass production.

Methods: We have shown earlier the delayed type hypersensitivity (DTH) assay could be used for evaluating the immunity and memory cells against specific Theileria antigen in vaccinated animals. In addition, TNF-α is the principle cytokine in modulating the cytotoxic activity of cytotoxic T-lymphocytes (CTL). Immunological analysis of the vaccine was performed by using two cell mediated immunity(CMI) in vitro and in vivo DTH test (Theilerin) and TNF-α assay.

Results: The results of immune responses of susceptible immunized cattle by bioreactor vaccine in comparison with conventional flask vaccine revealed a significant stimulation of immune cells by transcription of high level of TNF-α and positive reaction against Theileria antigen in Theilerin skin test (DTH).

Conclusion: The equal immunological results achieved in both above mentioned vaccines verified the satisfactory immunity for aerobic bioreactor theileriosis vaccine for advance mass vaccination in the field on a large scale


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How to Cite
Habibi G, Esmaeil-Nia K, Izadi H, Ataie Amarloie O, Afshari A, Bordbar N. Immunological Analysis of Aerobic Bioreactor Bovine Theileriosis Vaccine. Iran J Parasitol. 9(3):382-393.