Immunological Analysis of Aerobic Bioreactor Bovine Theileriosis Vaccine
Background: In this study, the pilot production of aerobic bioreactor tropical theileriosis vaccine was optimized with the aim of immunological assays for further mass production.
Methods: We have shown earlier the delayed type hypersensitivity (DTH) assay could be used for evaluating the immunity and memory cells against specific Theileria antigen in vaccinated animals. In addition, TNF-α is the principle cytokine in modulating the cytotoxic activity of cytotoxic T-lymphocytes (CTL). Immunological analysis of the vaccine was performed by using two cell mediated immunity(CMI) in vitro and in vivo DTH test (Theilerin) and TNF-α assay.
Results: The results of immune responses of susceptible immunized cattle by bioreactor vaccine in comparison with conventional flask vaccine revealed a significant stimulation of immune cells by transcription of high level of TNF-α and positive reaction against Theileria antigen in Theilerin skin test (DTH).
Conclusion: The equal immunological results achieved in both above mentioned vaccines verified the satisfactory immunity for aerobic bioreactor theileriosis vaccine for advance mass vaccination in the field on a large scale
Dolan TT. Theileriosis: a comprehensive review. Rev Sci Tech Off Int Epiz.1989; 8:11.
Brown CGD. Control of tropical theileriosis (Theileria annulata infection) of cattle. Parasitologia. 1990; 32:23–31.
Glass EJ, Innes EA, Spooner RL, Brown CGD. Infection of bovine monocyte/macrophage populations with Theileria annulata and Theileria parva. Vet Immunol Immunopathol. 1989; 22:355–368.
Hall FR. Antigens and immunity in Theileria annulata. Parasitol Today. 1988; 4(9):257-61.
Hashemi-Fesharki R. Chemotherapeutic value of parvaquone and buparvaquone against Theileria annulata infection of cattle. Res Vet Sci. 1991; 50(2):204-7.
Brown CGD. Application of in vitro cultures to vaccination against theileriosis. In Advances in the control of theileriosis (A.D. Irvin, M.P. Cunningham, A.S. Young, eds.). Martinus Nijhoff, The Hague, 1981. p. 104-119.
Brown CGD, Crawford JG, Kanhai GK, Njuguna LN, Stagg DA. Immunisation of cattle against East Coast fever with lymphoblastoid cell lines infected and transformed by Theileria parva. In Tick-borne diseases and their vectors (J.K.H. Wilde, ed.). Centre for Tropical Veterinary Medicine, Edinburgh, 1978. p. 331-333.
Pipano E. Bovine theileriosis in Israel. Rev Sci Tech Off Int Epiz. 1989; 8(1):79-87.
Hashemi-Fesharki R. Control of Theileria annulata in Iran. Parasitol Today. 1988; 4:36-40.
Singh DK, Thakur M, Raghav PRS, Varshney BC. Chemotherapeutic trials with four drugs in crossbred calves experimentally infected with Theileria annulata. Res Vet Sci. 1993; 54(1):68–71.
Stepanova NI, Zablotskii VT. Bovine theileriosis in the USSR. Rev Sci Tech Off Int Epiz. 1989; 8(1):89-92.
Innes EA, Millar P, Glass EJ, Brown CGD. In vitro infection of bovine alloreactive cytotoxic T-cell lines with sporozoites of T. annulata and T. parva. Res Vet Sci. 1989; 46: 367-374.
Preston PM, Brown CGD. Macrophage-mediated cytostasis and lymphocyte cytotoxicity in cattle immunized with Theileria annulata sporozoites or macroschizont-infected cell lines. Parasite Immunol. 1988; 10:631-647.
Hashemi-Fesharki R, Golchinfar F, Madani R, Esmaeilnia K. Comparative evaluation of antibody positive titer by ELISA and IFA in Theileria annulata vaccinated cattle in Iran. Parasite. 2006; 13(1):71-4.
Tait A, Hall FR. Theileria annulata: control measures, diagnosis and the potential use of subunit vaccines. Rev Sci Tech Off Int Epiz. 1990; 9(2):387-403
OIE - World Organization for Animal Health (International Office of Epizootics) 2008. Available: www.oie.int/fileadmin/Home/eng/Health.../ 2.04.16_THEILIERIOSIS.pdf
Mosmann TR, Coffman RL. TH1 and TH2 cells: different patterns of lymphokine secretion lead to different functional properties. Annu Rev Immunol. 1989; 7:145–173.
Broere F, Apasov SG, Sitkovsky MV, van Eden W. T cell subsets and T cell-mediated immunity. In Nijkamp FP, Parnham MJ. Principles of Immunopharmacology 3rd revised and extended edition. 2011. DOI 10.1007/978-3- 0346-0136-8_2, © Springer Basel AG 2011. p. 15-27
Derouich-Guergour D, Brenier-Pinchart MP, Ambroise-Thomas P, Pelloux H. Tumour necrosis factor alpha receptors: role in the physiopathology of protozoan parasite infections. Int J Parasitol. 2001; 31(8):763-9.
Prendergast BJ, Bilbo SD, Nelson RJ. Photoperiod controls the induction, retention, and retrieval of antigen-specific immunological memory. Am J Physiol Regul Integr Comp Physiol. 2004; 286(1):R54-60.
Habibi G, Bozorgi S, Hatami A, Esmaei-Nia K, Afshari A. Study on Cryo-preservation of Theileria annulata Schizont Infected Cell Line Vaccine Strain S15. Arch Razi Inst. 2012a; 67(2):167-172.
Bovine theileriosis Vaccine Master File (VMF), Razi Vaccine and Serum Research Institute, Karaj, Hessarak, Iran 1390.
Sambrook J, Fritsch EF, Maniatis T. Molecular Cloning: A Laboratory Manual, Volume 3, Cold Spring Harbor Laboratory, Cold Spring Harbor; NY, B.16. 1989.
Habibi G, Bozorgi S, Esmaeil-Nia K, Najjar E, Mohammadipour AR. Detection and Discrimination of Theileria annulata and Theileria lestoquardi by using a Single PCR. Arch Razi Inst. 2008; 63(1):47-52.
Habibi GR, Khamesipour A, McMaster WR, Mahboudi F. Cytokine Gene Expression in healing and Non-healing cases of cutaneous Leishmaniasis in response to in vitro stimulation with recombinant gp63 using sqRT-PCR Scand J Immunol. 2001; 54(4):414-20.
Habibi GR, Esmaeil-Nia K, Izadi H, Afshari A, Bozorgi S. Evaluation of Tumor Necrosis Factor Alpha (TNF-α) Gene Expression in Immunized Cattle with Two Types of Bovine theileriosis Vaccine Preparation. The 11th International Congress of Immunology and Allergy of Iran. Milad Tower, Tehran, Iran. 2012. 26-29 April.
Mallone R, Mannering SI, Brooks-Worrell BM, Durinovic-Belló I, Cilio CM, Wong FS, Schloot NC. Isolation and preservation of peripheral blood mononuclear cells for analysis of islet antigen- reactive T cell responses: position statement of the T-Cell Workshop Committee of the Immunology of Diabetes Society. Clin Exp Immunol. 2011; 163(1):33–49.
Habibi GR, Esmaeil-Nia K, Bozorgi S, Hashemi- Fesharki R, Bordbar N. Semi-quantitative Analysis of Expression of Various Genes in relation to Possible Markers for Theileria annulata Attenuation. Arch Razi Inst. 2009; 64(1):9-17
Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate- phenol-chloroform extraction. Anal Biochem. 1987; 162(1):156-9.
Balcerzak D, Querengesser L, Dixon W T, Baracos V E. Coordinate expression of matrixdegrading proteinases and their activators and inhibitors in bovine skeletal muscle. J Anim Sci. 2011; 79:94–107
Hashemi-Fesharki R, Habibi GR, Ahourai P. Delayed type hypersensitivity Theilerin test in cattle vaccinated against Theileria annulata infection. Vet Parasitol. 1998; 75(2-3):261-3.
Handman E, Chester PM, Remington JS. Delayed hypersensitivity to Toxoplasma and unrelated antigens in Toxoplasma-infected mice: induction and elicitation of delayed-type hypersensitivity by antigen-pulsed macrophages. Infect Immun. 1980; 28(2):524-31.
Oliveira F, Traoré B, Gomes R, et al. Delayedtype hypersensitivity to sand fly saliva in humans from a leishmaniasis-endemic area of Mali is Th1-mediated and persists to midlife. J Invest Dermatol. 2013; 133(2):452-9.
Mutiso JM, Macharia JC, Taracha E, Wafula K, Rikoi H, Gicheru MM. Safety and skin delayedtype hypersensitivity response in vervet monkeys immunized with Leishmania donovani sonicate antigen delivered with adjuvants. Rev Inst Med Trop Sao Paulo. 2012; 54(1):37-41.
de Waard R, Claassen E, Bokken GC, Buiting B, Garssen J, Vos JG. Enhanced immunological memory responses to Listeria monocytogenes in rodents, as measured by delayed-type hypersensitivity (DTH), adoptive transfer of DTH, and protective immunity, following Lactobacillus casei Shirota ingestion. Clin Diagn Lab Immunol. 2003; 10(1):59-65.
Miyauchi M, Murata M, Fukushima A, Sato T, Nakagawa M, Fujii T, Koseki N, Chiba N, Kashiwazaki Y. Optimization of cell-wall skeleton derived from Mycobacterium bovis BCG Tokyo 172 (SMP-105) emulsion in delayed-type hypersensitivity and antitumor models. Drug Discov Ther. 2012; 6(4):218-25.
Namavari MM, Shad-Del F, Hosseini SMH, Hashemi-Fesharaki R, Seghatol-Eslam A. A survey on delayed hypersensitivity in the sheep immunized against theileriosis. Iran J Vet Res. University of Shiraz. 2004; 5(2)10:102-104.
Stewart VA, Walsh DS, McGrath SM, et al. Cutaneous delayed-type hypersensitivity (DTH) in a multi-formulation comparator trial of the antifalciparum malaria vaccine candidate RTS,S in rhesus macaques. Vaccine. 2006; 24(42-43): 6493-502.
Preston PM, Brown CGD, Spooner RL. Cellmediated cytotoxicity in Theileria annulata infections of cattle with evidence for BoLA restriction. Clin Exp Immunol. 1983; 53:88-100.
Taracha ELN, Goddeeris BM, Teale AJ, Kemp SJ, Morrison WI. Parasite strain specificity of bovine cytotoxic T cell responses to Theileria parva is determined primarily by immunodominance. J Immunol. 1995; 155:4854-4860.
Ahmed JS, Rothert M, Steuber S, Schein E. In vitro proliferative and cytotoxic responses of PBL from Theileria annulata-immune cattle. JVet Med (B). 1989; 36:584–592.
Preston PM, Brown CG, Richardson W. Cytokines inhibit the development of trophozoiteinfected cells of Theileria annulata and Theileria parva but enhance the proliferation of macroschizont- infected cell lines. Parasite Immunol. 1992; 14:125–141.
Visser AE, Abraham A, Sakyi LJ, Brown CG, Preston PM. Nitric oxide inhibits establishment of macroschizont-infected cell lines and is produced by macrophages of calves undergoing bovine tropical theileriosis or East Coast fever. Parasite Immunol. 1995; 17:91–102.
Campbell JD, Brown DJ, Nichani AK, Howie SE, Spooner RL, Glass EJ. A non-protective T helper 1 response against the intra-macrophage protozoan Theileria annulata. Clin Exp Immunol. 1997; 108(3):463-70.
Denkers EY, Gazzinelli RT. Regulation and Function of T-Cell-Mediated Immunity during Toxoplasma gondii Infection. Clin Microbiol Rev. 1998; 11(4):569-88.
Darghouth MA. Review on the experience with live attenuated vaccines against tropical theileriosis in Tunisia: considerations for the present and implications for the future. Vaccine. 2008; 26 (Suppl 6):G4-G10.
Pipano E, Tsur I. Experimental immunisation against Theileria annulata using a tissue culture vaccine. Refuah Veterinarith. 1966; 23:186–94.
Zhang ZH. Theileria annulata and its control in China. In: Singh DK, Varshney BC, editors. Proceedings of the second EEC workshop on orientation and coordination of research on tropical theileriosis. Anand, India: National Dairy Development Board; 1991. p. 11–14.,
Guo G, Shang J, Yu M, Zhao J, Ma S, Ali N, et al. Research on the schizonts cell culture vaccine against Theileria annulata infection in Xinjiang, China. Trop Anim Health Prod. 1997; 29(Supplement):98S–100S.
Boulter NR, Hall R. Immunity and vaccine development in the bovine theileriosis. Adv Parasitol. 2000; 44:41–97.
Darghouth MA, Ben Miled L, Bouattour A, Melrose TR, Brown CGD, Kilani M. A preliminary study on the attenuation of Tunisian infected cell lines of Theileria annulata. Parasitol Res. 1996; 82:647–55.
Onar E, Alp HG. Immunisation of cattle against Theileria annulata using different numbers of attenuated vaccine cells. In: Singh DK, Varshney BC, editors. Proceedings of the second EEC workshop on orientation and coordination of research on tropical theileriosis. Anand, India: National Dairy Development Board; 1991. p. 55–59.
Darghouth MA, Boulter N, Gharbi M, Sassi L, Tait A, Hall R. Vaccination of calves with and an attenuated cell line of Theileria annulata and the sporozoite antigen SPAG1 produces a synergistic effect. Vet Parasitol. 2006; 142:54–62.
Pipano E, Weisman Y, Benado A. Virulence of four local strains of Theileria annulata. Refuah Veterinarith. 1974; 31(2):59-63.
McKeever DJ. Bovine immunity – a driver for diversity in Theileria parasites? Trends Parasitol. 2009; 25(6): 269-276.
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