Molecular Identification and Phylogenetic Analysis of Leishmania major Isolated from Zoonotic Cutaneous Leishmaniasis Foci in Northeast Iran
Abstract
Background: Epidemiological studies, classification and genetic studies of Leishmania species are effective in treatment, control and prevention in endemic areas. We aimed to investigate the genetic diversity and phylogeny of Leishmania in Zoonotic foci located in northeastern Iran using nagt gene for the first time.
Method: DNA of 100 confirmed positive slides collected from the health centers of Sarkhes, Darghez, Fariman, Esfarayen, and Sabzevar were extracted during 2020-2021. The partial sequence of kDNA was amplified to identify the species. Twenty-five DNA samples were randomly subjected to amplify by nagt gene primes and were sequenced. The sequences were aligned with reference sequences in National Center for Biotechnology Information (NCBI). Then, the genetic similarities of the sequences were checked using Clustalx2.1 software and the phylogenetic tree was drawn by Mega 7 software.
Result: All the positive samples were diagnosed as L. major. Approximately, half of the sequences of species were similar to two reference genes JX103550.1:404-712 L. major Esfahan and KX759012.1:568-807 L. Major Ilam (more than 90% similarity). According to the results of the phylogeny tree, the closest genotype to our study samples was JX103550.1:404-712 L. major Esfahan.
Conclusion: The most causative agent CL in these areas was L. major. The genetic diversity of L. major was high such as other zoonotic foci in Iran. Due to the high similarity of the strains in the study areas with the strains of Isfahan and Ilam, similar control and prevention methods is suggested in these areas.
2. Bamorovat M, Sharifi I, Afshari SAK, et al. Poor adherence is a major barrier to the proper treatment of cutaneous leishmaniasis: A case-control field assessment in Iran. Int J Parasitol Drugs Drug Resist. 2023;21:21-27.
3. Mazaherifar S, Solhjoo K, Rasti S, Heidarnejadi SM, Abdoli A. Patterns of cutaneous leishmaniasis during the covid-19 pandemic in four endemic regions of Iran. Trans R Soc Trop Med Hyg. 2023;117:38-44.
4. Wamai RG, Kahn J, McGloin J, Ziaggi G. Visceral leishmaniasis: A global overview. Journal of Global Health Science. 2020;2
5. Jalali H, Enayati AA, Fakhar M, et al. Reemergence of zoonotic cutaneous leishmaniasis in an endemic focus, northeastern Iran. Parasite Epidemiol Control. 2021;13:e00206.
6. Jafari R, Abdoli H, Arandian MH, et al. Emerging of cutaneous leishmaniais due to Leishmania major in a new focus in esfahan province, central Iran. J Arthropod Borne Dis. 2020;14:134-143.
7. Ulusan Ö, Mert U, Sadıqova A, Öztürk S, Caner A. Identification of gene expression profiles in Leishmania major infection by integrated bioinformatics analyses. Acta Trop. 2020;208:105517.
8. El-Beshbishy HA, Al-Ali KH, El-Badry AA. Molecular characterization of cutaneous leishmaniasis in al-madinah al-munawarah province, western saudi arabia. Int J Infect Dis. 2013;17:e334-e338.
9. Farrokhi-Karibozorg M, Hajjaran H, Ghayour-Najafabadi Z, Hejazi SH, Ataei-Pirkooh A, Mohebali M. Identification and phylogenic analysis of Leishmania species among patients and reservoir hosts based on n-acetylglucosamine-1-phosphate transferase gene in central Iran. Ann Parasitol. 2022;68:461-471.
10. Waki K, Dutta S, Ray D, et al. Transmembrane molecules for phylogenetic analyses of pathogenic protists: Leishmania-specific informative sites in hydrophilic loops of trans-endoplasmic reticulum n-acetylglucosamine-1-phosphate transferase. Eukaryot Cell. 2007;6:198-210.
11. Ghatee MA, Taylor WR, Karamian M. The geographical distribution of cutaneous leishmaniasis causative agents in Iran and its neighboring countries, a review. Front Public Health. 2020;8:11.
12. Hajjaran H, Mohebali M, Teimouri A, et al. Identification and phylogenetic relationship of Iranian strains of various Leishmania species isolated from cutaneous and visceral cases of leishmaniasis based on n-acetylglucosamine-1-phosphate transferase gene. Infect Genet Evol. 2014;26:203-212.
13. Sarkari B, Ahmadpour NB, Motazedian MH, et al. Inter-and intraspecific variations of Leishmania strains isolated from patients with cutaneous and visceral leishmaniases in Fars province, south of Iran. Iran J Med Sci. 2016;41:209.
14. Chaouch M, Fathallah-Mili A, Driss M, et al. Identification of tunisian Leishmania spp. by PCR amplification of cysteine proteinase b (cpb) genes and phylogenetic analysis. Acta Trop. 2013;125:357-365.
15. Fakhar M, Ghohe HP, Rasooli SA, et al. Genetic diversity of Leishmania tropica strains isolated from clinical forms of cutaneous leishmaniasis in rural districts of herat province, western afghanistan, based on its1-rdna. Infect Genet Evol. 2016;41:120-127.
16. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. Mega5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol. 2011;28:2731-2739.
17. Saberi R, Moin-Vaziri V, Hajjaran H, et al. Identification of Leishmania species using n-acetylglucosamine-1-phosphate transferase gene in a zoonotic cutaneous leishmaniasis focus of Iran. J Vector Borne Dis. 2018;55:14-19.
18. Karami M, Doudi M, Setorki M. Assessing epidemiology of cutaneous leishmaniasis in Isfahan, Iran. J Vector Borne Dis. 2013;50:30-37.
19. Mohammadiha A, Dalimi A, Mohebali M, et al. Molecular identification and phylogenetic classification of Leishmania spp. Isolated from human cutaneous leishmaniasis in Iran: A cross-sectional study. Iran J Parasitol. 2018;13:351-61.
20. Farash BRH, Shamsian SAA, Mohajery M, et al. Changes in the epidemiology of cutaneous leishmaniasis in northeastern Iran. Turkiye Parazitolojii Dergisi. 2020;44:52-57.
21. Mahnaz T, Al-Jawabreh A, Kuhls K, Schönian G. Multilocus microsatellite typing shows three different genetic clusters of Leishmania major in Iran. Microb Infect. 2011;13:937-942.
22. Mahmoudzadeh‐Niknam H, Ajdary S, Riazi‐Rad F, et al. Molecular epidemiology of cutaneous leishmaniasis and heterogeneity of Leishmania major strains in Iran. Trop Med Int Health. 2012;17:1335-1344.
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Issue | Vol 18 No 3 (2023) | |
Section | Original Article(s) | |
DOI | https://doi.org/10.18502/ijpa.v18i3.13752 | |
Keywords | ||
Leishmania Zoonotic cutaneous leishmaniasis Phylogeny Northeastern Iran |
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