Protoscolecidal Effect of Berberis vulgaris Root Extract and Its Main Compound, Berberine in Cystic Echinococcosis
-
Hossein Mahmoudvand
,
Ebrahim Saedi Dezaki
,
Fariba Sharififar
,
Behrouz Ezatpour
,
Sareh Jahanbakhsh
,
Majid Fasihi Harandi
Abstract
Background: Cystic echinococcosis (CE), a zoonotic parasitic infection caused by the metacestode (larvae) stage of dog tapeworm Echinococcus granulosus and recognized as a major economic and public health concern in the world. This study aimed to investigate the in vitro scolicidal effect of methanolic extract of Berberis vulgaris L. roots and its main compound, berberine against protoscoleces of hydatid cysts.
Methods: For this purpose, protoscoleces were aseptically aspirated from sheep livers having hydatid cysts. Various concentrations of the methanolic extract (0.25-2 mg/ml) and berberine (0.062- 0.5 mg/ml) were used for 5 to 30 min. Viability of protoscoleces was confirmed by eosin exclusive test.
Results: In the present study, all of the various concentrations of the B. vulgaris methanolic extract (0.25, 0.5, 1 and 2 mg/ml) and berberine (0.062, 0.125, 0.25 and 0.5 mg/ml) revealed significant (P<0.05) scolicidal effects against protoscoleces of E. granulosus in a dose-dependent manner. Both berberine and methanolic extract exhibited 100% inhibition against protoscoleces of E. granulosus at the concentration of 2.0 and 0.5 mg/ml after 10 min incubation respectively.
Conclusion: According to the results, both B. vulgaris methanolic extract and berberine alone demonstrated high scolicidal activities against protoscoleces of hydatid cysts in low concentration and short exposure time on in vitro model.However, in vivo efficacy of B. vulgaris and berberine also requires to be evaluated using an animal model with hydatid infection.
Fasihi Harandi M, Budke CM, Rostami S. The Monetary Burden of Cystic Echinococcosis in Iran. PLOS Negl Trop Dis. 2012; 6(11) e1915.
Torgerson PR, Budke CM. Echinococcosis – an international public health challenge. Res Vet Sci. 2003; 74: 191-202.
Eckert J, Deplazes P. Biological, epidemiological, and clinical aspects of echinococcosis, a zoonosis of increasing concern. Clin Microbiol Rev. 2004; 17: 107-35.
Brunetti E, Kern P, Vuitton DA. Writing Panel for the WHO-IWGE. Expert consensus for the diagnosis and treatment of cystic and alveolar echinococcosis in humans. Acta Trop. 2010; 114(1):1-16.
Rajabi MA. Fatal reactions and methaemoglobinaemia after silver nitrate irrigation of hydatid cyst. Surg Pract. 2009; 13: 2-7.
Topcu O, Sumer Z, Tuncer E, Aydin C, Koyuncu A. Efficacy of chlorhexidine gluconate during surgery for hydatid cyst. World J Surg. 2009; 33: 1274–80.
McManus DP, Zhang W, Li J. Echinococcosis. Lancet. 2003; 362:1295-304.
Adas G, Arikan S, Kemik O, Oner A, Sahip N, Karatepe O. Use of albendazole sulfoxide, albendazole sulfone, and combined solutions as scolicidal agents on hydatid cysts (in vitrostudy). World J Gastroenterol. 2009; 15: 112-6.
Zibaei M, Sarlak A, Delfan B, Ezatpour B, Azargoon A. Scolicidal effects of Olea europaea and Satureja khuzestanica extracts onprotoscolices of hydatid cysts. Korean J Parasitol. 2012; 50(1): 53-6.
Rocha LG, Almeida JR, Macedo RO, Barbosa-Filho JM. A review of natural products with antileishmanial activity. Phytomedicin. 2005; 12: 514-35.
Imanshahidi H, Hosseinzadeh H. Pharmacological and therapeutic effects of Berberis vulgaris and its active constituent, Berberine. Phytother Res. 2008; 22: 999-1012.
Ivanovska N, Philipov S. Study of the antiinflammatory action of Berberis vulgaris root extract, alkaloid fractions and pure alkaloids. Int J Immunopharmacol. 1996; 18: 553-61.
Küpeli E, Koar M, Yeilada E, Hüsnü K, Baer C. A comparative study on the antiinflammatory, antinociceptive and antipyretic effects of isoquinoline alkaloids from the roots of Turkish Berberis species. Life Sci. 2002; 72: 645-7.
Nakamoto K, Sadamori S, Hamada T. Effects of crude drugs and berberine hydrochloride on the activities of fungi. J Prosthet Dent. 1990; 64(6): 691-4.
Freile ML, Giannini F, Pucci G, Sturniolo A, Rodero L, Pucci O. Antimicrobial activity of aqueous extracts and of berberine isolated from Berberis heterophylla. Fitoterapia. 2003;74:702-5.
Ghaderi R, Maleki Nejad P. evaluation of anticandidal effects of Berberis vulgaris root extracts (methanolic and aqueous) and comparing their effects with those clotrimazole. J Birjand Univ Med Sci. 2006; 13(2): 42-8.
Vennerstrom JL, Lovelace JK, Waits VB, Hanson WL, Klayman DL. Berberine derivatives as anti-leishmanial drugs. Antimicrob Agents Chemother. 2005; 34(5): 198-211.
Kaneda Y, Torii M, Tanaka T, Aikawa M. In vitro effects of berberine sulphate on the growth and structure of Entamoeba histolytica, Giardia lamblia and Trichomonas vaginalis. Ann Trop Med Parasitol. 1991; 85: 417-25.
Fata A, Rakhshandeh H, Berenji F, Jalalifard A. Ttreatment of cutaneous leishmaniasis in murine model by alcoholic extract of Berberis vulgaris. Iran J Parasitol. 2006; 1(1): 39-42.
Mahmoudvand H, Sharififar F, Sharifi I, Ezatpour B, Fasihi Harandi M, Makki M, Ziaali N, Jahanbakhsh S. In vitro inhibitory effect of Berberis vulgaris (Berberidaceae) and its main component, berberine against different Leishmania species. Iran J Parasitol. 2014; 9(1):28-36.
Sharififar F, Mozaffarian V, Moshafi MH. Chemical composition and biological activities of Zhumeria majdae Resh. f. &Wendelbo. Jundishapur J Nat Pharm Prod. 2008; 3(1): 8-18.
Moazeni M, Saharkhiz MJ, Hosseini AA. In vitro lethal effect of ajowan (Trachyspermum ammi L.) essential oil on hydatid cyst protoscoleces. Vet Parasitol. 2012; 187: 203-8.
Smyth JD, Barrett NJ. Procedures for testing the viability of human hydatid cysts following surgical removal, especially after chemotherapy. Trans R Soc Trop Med Hyg. 1980; 74: 649-52.
Moro PL, Schantz PM. Echinococcosis:historical landmarks and progress in research and control. Ann Trop Med Parasitol. 2006; 100:703-14.
Kayaalp C, Balkan M, Aydin C, Ozgurtas T, Tanyuksel M, Kirimlioglu V, Akoglu M, Oner K, Pekcan M. Hypertonic saline in hydatid disease. World J Surg. 2001; 25: 975-9.
Caglar R, Yuzbasioglu MF, Bulbuloglu E, Gul M, Ezberci F, Kale I. In vitro effectiveness of different chemical agents on scolices of hydatid cyst. J Invest Surg. 2001; 21:71-5
Besim H, Karayalcin K, Hamamci O, Güngör C, Korkmaz A. Scolicidal agents in hydatid cyst surgery. HPB Surg. 1998; 10: 347-51.
Erzurumlu K, Hokelek M, Baris S, Sahin M, Birinci A, Amanvermez R, Tac K. Effect of albendazole sulfoxide solution on the scolices and the hepatobiliary system. Eur Surg Res. 1998; 30: 433-8.
Landa Garcı´a JI, Alonso E, Gonzalez-Uriarte J. Evaluation of scolicidal agents in an experimental hydatid disease model. Eur Surg Res. 1997; 29: 202-8.
Paksoy Y, Odev K, Sahin M. Percutaneous treatment of hydatid cysts: comparison of direct injection of albendazole and hypertonic saline solution. AJR Am J Roentgenol. 2005; 185(3): 727-34.
Puryan K, Karadayi K, Topcu O. Chlorhexidine gluconate: an ideal scolicidal agent in the treatment of intraperitoneal hydatidosis. World J Surg. 2005; 29: 227-30.
Kilicoglu B, Kismet K, Koru O. Tanyuksel M, Oruc MT, Sorkun K, Akkus MA. The scolicidal effects of honey. Adv Ther. 2006; 23: 1077-83.
Mahmoudvand H, Fasihi Harandi M, Shakibaie M, Aflatoonian MR, Makki MS, Jahanbakhsh S. Scolicidal effects of biogenic selenium nanoparticles against protoscolices of hydatid cysts. Int J Surg. 2014 Mar 28. doi: 10.1016/j. ijsu.2014.03.017. [Epub ahead of print].
Cowan MM. Plant products as antimicrobial agents. Clin Microb Rev. 1999; 12: 564-82.
Jones FA. Herbs-useful plants. Their role in history and today. Eur J Gastroenterol Hepatol. 1996; 8: 1227-31.
McCutcheon AR, Ellis SM, Hancock REW, Tower GN. Antibiotic screening of medicinal plants of the British Columbian native peoples. J Ethnopharmacol. 1992; 37: 213-23.
Rouhani S, Salehi N, M Kamalinejad M, Zayeri F. Efficacy of Berberis vulgaris aqueous extract
on viability of Echinococcus granulosus protoscolices. J Invest Surg. 2013; 26(6): 347-51.
Peychev L. Pharmacological investigation on the cardiovascular effects of Berberis vulgaris on tested animals. Pharmacia. 2005; 52: 118-21.
Lin CC, Ng LT, Hsu FF, Shieh DE, Chiang LC. Cytotoxic effects of Coptis chinensis and
Epimedium sagittatum extracts and their major constituents (berberine, coptisine and icariin)on hepatoma and leukaemia cell growth. Clin Exp Pharmacol Physiol. 2004; 31: 65-9.
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| Issue | Vol 9 No 4 (2014) | |
| Section | Articles | |
| Keywords | ||
| Echinococcus granulosus European barberry Hydatid cyst Scolicidal | ||
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