Inhibitory Effects of Leishmania Mexicana infection on MHC-I Expression in Bone Marrow Derived Dendritic Cells
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Abstract
Background: Leishmania is a parasite causing leishmaniasis with different clinical manifestations depending on the infectious species in many countries worldwide. Although different studies have been taken place to clear the interaction of the parasite with the immune system, many aspects of immunology of leishmaniasis is remained uncertain.
Methods: Bone marrow derived dendritic cells (DCs) were cultured in vitro and divided into different groups (Nottingham Trent University, Nottingham, UK). The groups were separately infected with live or autoclaved L. mexicana or loaded with Soluble Leishmania Antigen (SLA). The expression of major histocompatibility complex class I (MHC-I) molecule was checked and compared on the cultured DCs using flow cytometry.
Results: Infection of L. mexicana caused a significant downregulation in expression of molecules where killed Leishmania or SLA could not induce suppression in expression of these molecules.
Conclusion: L. mexicana infection results in downregulation of MHC-I expression on bone marrow-derived dendritic cells.
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18. Tiburcio R, Nunes S, Nunes I, et al. Molecular Aspects of Dendritic Cell Activation in Leishmaniasis: An Immunobiological View. Front Immunol. 2019;10:227.
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23. Prina E, Jouanne C, de Souza Lao S, et al. Antigen presentation capacity of murine macrophages infected with Leishmania amazonensis amastigotes. J Immunol. 1993;151(4):2050-61.
24. Meier CL, Svensson M, Kaye PM. Leishmania-induced inhibition of macrophage antigen presentation analyzed at the single-cell level. J Immunol. 2003;171(12):6706-13.
25. Argueta Donohué J, Wilkins Rodríguez AA, Aguirre García M, Gutiérrez Kobeh L. Differential phagocytosis of Leishmania mexicana promastigotes and amastigotes by monocyte derived dendritic cells. Microbiol Immunol. 2016;60(6):369-81.
26. Contreras I, Estrada JA, Guak H, et al. Impact of Leishmania mexicana infection on dendritic cell signaling and functions. PLoS Negl Trop Dis. 2014;8(9):e3202.
2. Al-Ashmawy G. Dendritic cell subsets, maturation and function. Dendritic Cells. 2018;2018:11-24.
3. Rivera-Fernández I, Argueta-Donohué J, Wilkins-Rodríguez AA, Gutiérrez-Kobeh L. Effect of two different isolates of Leishmania mexicana in the production of cytokines and phagocytosis by murine dendritic cells. J Parasitol. 2019;105(2):359-70.
4. Favali C, Tavares N, Clarencio J, Barral A, Barral-Netto M, Brodskyn C. Leishmania amazonensis infection impairs differentiation and function of human dendritic cells. J Leukoc Biol. 2007;82(6):1401-6.
5. Weiss DJ, Evanson OA, McClenahan DJ, Abrahamsen MS, Walcheck BK. Regulation of expression of major histocompatibility antigens by bovine macrophages infected with Mycobacterium avium subsp. paratuberculosis or Mycobacterium avium subsp. avium. Infect Immun. 2001;69(2):1002-8.
6. Caspar-Bauguil S, Puissant B, Nazzal D, et al. Chlamydia pneumoniae induces interleukin-10 production that down-regulates major histocompatibility complex class I expression. J Infect Dis. 2000;182(5):1394-401.
7. Antoniou AN, Powis SJ. Pathogen evasion strategies for the major histocompatibility complex class I assembly pathway. Immunol. 2008;124(1):1-12.
8. Deitz SB, Dodd DA, Cooper S, Parham P, Kirkegaard K. MHC I-dependent antigen presentation is inhibited by poliovirus protein 3A. Proc Natl Acad Sci USA. 2000;97(25):13790-5.
9. Neumeister B, Faigle M, Spitznagel D, Mainka A, Ograbek A, Wieland H, et al. Legionella pneumophila down-regulates MHC class I expression of human monocytic host cells and thereby inhibits T cell activation. Cell Mol Life Sci. 2005;62(5):578-88.
10. Ali SA, Rezvan H, McArdle SE, Khodadadi A, Asteal FA, Rees RC. CTL responses to Leishmania mexicana gp63-cDNA vaccine in a murine model. Parasite Immunol. 2009;31(7):373-83.
11. Arango Duque G, Descoteaux A. Macrophage cytokines: involvement in immunity and infectious diseases. Front Immunol. 2014;5:491.
12. Dumonteil E, Maria Jesus RS, Javier EO, Maria del Rosario GM. DNA vaccines induce partial protection against Leishmania mexicana. Vaccine. 2003;21(17-18):2161-8.
13. Inaba K, Inaba M, Romani N, et al. Generation of large numbers of dendritic cells from mouse bone marrow cultures supplemented with granulocyte/macrophage colony-stimulating factor. J Exp Med. 1992;176(6):1693-702.
14. Bennett CL, Misslitz A, Colledge L, Aebischer T, Blackburn CC. Silent infection of bone marrow-derived dendritic cells by Leishmania mexicana amastigotes. Eur J Immunol. 2001;31(3):876-83.
15. Lucas M, Karrer U, Lucas A, Klenerman P. Viral escape mechanisms--escapology taught by viruses. Int J Exp Pathol. 2001;82(5):269-86.
16. Silverman JM, Clos J, Horakova E, et al. Leishmania exosomes modulate innate and adaptive immune responses through effects on monocytes and dendritic cells. J Immunol. 2010;185(9):5011-22.
17. Luder CG, Lang T, Beuerle B, Gross U. Down-regulation of MHC class II molecules and inability to up-regulate class I molecules in murine macrophages after infection with Toxoplasma gondii. Clin Exp Immunol. 1998;112(2):308-16.
18. Tiburcio R, Nunes S, Nunes I, et al. Molecular Aspects of Dendritic Cell Activation in Leishmaniasis: An Immunobiological View. Front Immunol. 2019;10:227.
19. Roy K, Naskar K, Ghosh M, Roy S. Class II MHC/peptide interaction in Leishmania donovani infection: implications in vaccine design. J Immunol. 2014;192(12):5873-80.
20. Chakraborty D, Banerjee S, Sen A, Banerjee KK, Das P, Roy S. Leishmania donovani affects antigen presentation of macrophage by disrupting lipid rafts. J Immunol. 2005;175(5):3214-24.
21. Roy K, Mandloi S, Chakrabarti S, Roy S. Cholesterol Corrects Altered Conformation of MHC-II Protein in Leishmania donovani Infected Macrophages: Implication in Therapy. PLoS Negl Trop Dis. 2016;10(5):e0004710.
22. Kima PE, Soong L, Chicharro C, Ruddle NH, McMahon-Pratt D. Leishmania-infected macrophages sequester endogenously synthesized parasite antigens from presentation to CD4+ T cells. Eur J Immunol. 1996;26(12):3163-9.
23. Prina E, Jouanne C, de Souza Lao S, et al. Antigen presentation capacity of murine macrophages infected with Leishmania amazonensis amastigotes. J Immunol. 1993;151(4):2050-61.
24. Meier CL, Svensson M, Kaye PM. Leishmania-induced inhibition of macrophage antigen presentation analyzed at the single-cell level. J Immunol. 2003;171(12):6706-13.
25. Argueta Donohué J, Wilkins Rodríguez AA, Aguirre García M, Gutiérrez Kobeh L. Differential phagocytosis of Leishmania mexicana promastigotes and amastigotes by monocyte derived dendritic cells. Microbiol Immunol. 2016;60(6):369-81.
26. Contreras I, Estrada JA, Guak H, et al. Impact of Leishmania mexicana infection on dendritic cell signaling and functions. PLoS Negl Trop Dis. 2014;8(9):e3202.
| Files | ||
| Issue | Vol 17 No 4 (2022) | |
| Section | Original Article(s) | |
| DOI | https://doi.org/10.18502/ijpa.v17i4.11284 | |
| Keywords | ||
| Bone marrow Dendritic cells Leishmania mexicana MHC-I Peptide | ||
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How to Cite
1.
Rezvan H, Ali S, Hamoon Navard S. Inhibitory Effects of Leishmania Mexicana infection on MHC-I Expression in Bone Marrow Derived Dendritic Cells. Iran J Parasitol. 2022;17(4):562-572.
