Original Article

Anti-Toxoplasma Activity of 2-(Naphthalene-2-γlthiol)-1H Indole

Abstract

Background:This study was undertaken to evaluate the viability, infectivity and immunity of Toxoplasma gondii tachyzoites exposed to 2-(naphthalene-2-ylthio)-1H-indole.
 Methods:Tachyzoites of RH strain were incubated in various concentrations of 2- (naphthalene-2-ylthio)-1H-indole (25-800μM) for 1.5 hours. Then, they were stained by PI and analyzed by Fluorescence-activated cell sorting (FACS). To eval-uate the infectivity, the tachyzoites exposed to the different concentrations of the compound were inoculated to 10 BALB/c mice groups. For Control, parasites ex-posed to DMSO (0.2% v/v) were also intraperitoneally inoculated into two groups of mice. The immunity of the exposed tachyzoites was evaluated by inoculation of the naïve parasite to the survived mice.
Results:The LD50 of 2-(naphthalene-2-ylthio)-1H-indole was 57 μmol. The lon-gevity of mice was dose dependent. Five mice out of group 400μmol and 3 out of group 800μmol showed immunization to the parasite.
 Conclusion:Our findings demonstrated the toxoplasmocidal activity of the com-pound. The presence of a well-organized transporter mechanism for indole com-pounds within the parasite in conjunction with several effective mechanisms of these compounds on Toxoplasma viability would open a window for production of new drugs and vaccines.

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SectionOriginal Article(s)
Keywords
2-(naphthalene-2-ylthio)-1H-indole Immunity Infectivity Toxoplasma gondii Viability
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1.
Asgari Q, Keshavarz H, Rezaeian M, Sadeghpour H, Miri R, Motazedian MH. Anti-Toxoplasma Activity of 2-(Naphthalene-2-γlthiol)-1H Indole. Iran J Parasitol. 1;10(2):171-180.