Phylogenetic Study of Haemonchus Species from Iran Based On Morpho-Molecular Characterization
Background:Haemonchosis has a negative effect on the farming industry throughout the world, especially in the tropic and sub-tropic countries. The present study was carried out to differentiate Haemonchus species from its main hosts in Iran, including sheep, goat and camel.
Methods:The identification took place based on the morphometrics of the spic-ules and molecular characters. Two hundred seventy adult male nematodes were collected from the abomasums of different ruminants (90 samples from each ani-mal) at the slaughterhouses from different localities in Iran. Samples were morpho-logically identified according to the spicules’ morphometric measurements. In the section on molecular study, 10 samples of each Haemonchus isolates were genetically examined. A simple PCR-restriction fragment length polymorphism (PCR-RFLP) assay of the second internal transcribed spacer of ribosomal DNA (ITS2-rDNA) were described to confirm the PCR results.
Results:PCR-RFLP profile obtained from the restriction enzyme HPa1 in H. con-tortus and H. longistipes indicated 1 (278 bp) and 2 (113 and 135 bp) different frag-ments, respectively. The morphological parameters clearly distinguish H. contortus from H. longistipes. Moreover, regarding the ITS2-rDNA, sequences of 295 bp and 314 bp were obtained from H. contortus and H. longistipes, respectively.
Conclusion:The genotypic results are in agreement with the phenotypic findings of both species.
Nabavi R, Eslami A, ShoKrani HR, Bokaie S, Shayan P, Saadati D. Study on the prevalence, intensity, seasonal dynamic of abomasal hel-minths in sheep from different climatic zones of Iran. World Appl Sci J. 2011; 12(4): 441-445.
Samson-Himmelstjerna G, Harder A, Schnieder T. Quantitive analysis of ITS2 se-quences in trichostringyle parasites. Int J Parasi-tol. 2001; 32: 1529-1535.
Heise M, Epe C, Schnider T. Differences in the second internal transcribed spacer (ITS-2) of eight species of gastrointerstinal nematodes of ruminants. J Parasitol. 1999; 85(3): 431-435.
Dallas JF, Irvine RJ, Halvorsen O. DNA evi-dence that Marshallagia marshalli Ransom, 1907 and M. occidentalis Ransom, 1907. (Nematoda: Ostertagiinae) from Svacibard reindeer are con-specific. Sys Parasitol. 1907; 50: 101-103.
Dallas JF, Irvine RJ, Halvorsen O, Albon SD. Identification by polymerase chain reaction (PCR) of Marshallagia marshalli and Ostertagia gruehneri from Svalbard reindeer. Int J Parasitol. 2000; 30: 863-866.
Abramatov MB, Amirov OO, Euchboev AE, Khalilov IM, Abdurakhmanov IY. Morpholog-ical and molecular characterization of Haemon-chus contortus and H. placei (Nematoda: Tricho-strongylidae) from Uzbekistan by sequences of the second internal transcribed spacer of ribo-somal DNA. Sci Parasitol. 2013; 14: 115-120.
Lichtenfels JR, Pilitt PA, Le Jambre LF. Cu-ticular ridge patterns of Haemonchus contortus and Haemonchus placei (Nematoda: Trichostrongy-loidea). Proc Helminthol Soc Washington. 1985; 53: 94-101.
Lichtenfels JR, Pilitt PA, Le Jambre LF. Spicule lengths of the ruminant stomach nematodes Haemonchus contortus, Haemonchus placei, and their hybrids. Proc Helminthol Soc Washington. 1988; 55: 97-100.
Lichtenfels JR, Pilitt PA, Hoberg EP. New morphological characters for identifying indi-vidual speciments of Haemonchus spp. (Nema-toda: Trichostrongyloidea) and a key to speciesin ruminants of North America. J Parasitol. 1994; 80: 107-119.
Chilton NB, Gasser RB, Beveridge I. Differ-ences in a ribosomal DNA sequence of mor-phologically indistinguishable species within the Hypodontus macropi complex (Nematoda: Stron-gyloidea). Int J Parasitol. 1995; 25: 647-651.
Gasser RB, Hung GC, Chilton NB, Beveridge I. Advance in developing molecular diagnostic tools for strongyloid nematodes of equids: fundamental and applied implication. Mol Cell Prob. 2004; 8: 3-16.
Gasser RB, Newton SE. Genomic and genetic research on bursate nematodes: significance, implication and prospects. Int J Parasitol. 2000; 30: 509-534.
Meshgi B, Saraian I, Mahmoodpor D, Mor-tazavi A. Helminthic parasites fauna of alimen-tary canal of sheep and goat in Shahrekord, Iran. Iran Vet J. 2006; 2(2): 82-87.
Eslami A, Nabavi L. Species of gastrointestinal nematodes of sheep from Iran. Bull Soc Pathol Exo. 1976; 69 (1): 92-95.
Eslami A, Meydani M, Maleki S, Zargarzadeh A. Gastrointestinal nematodes of wild sheep (Ovis orientalis) from Iran. J Wildl Dis. 1976; 15: 265-263.
Stevenson LA, Chilton NB, Gasser B. Differ-entiation of Haemonchus placei from H. contortus (Nematoda: Trichostrogylidae) by the riboso-mal DNA second internal transcribed spacer. Int J Parasitol. 1995; 24(4): 483-488.
|Issue||Vol 10 No 2 (2015)|
|Haemonchus species ITS2 Morphology PCR-RFLP|
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