Detection of Blastocystis Subtypes in Children with Functional Abdominal Pain and Celiac Disease in Çorum, Turkey
-
Ayşe Semra Gureser
,
Atakan Comba
,
Djursun Karasartova
,
Nezahat Koşar
,
Adem Keskin
,
Christen Rune Stensvold
,
Ayşegül Taylan-Ozkan
Abstract
Background: Blastocystis has been associated with various symptoms of the gastrointestinal tract. We aimed to investigate the prevalence of Blastocystis in children with celiac disease (CeD) or functional abdominal pain (FAP) and to evaluate its subtypes (STs) with respect to demographic, socioeconomic and epidemiological factors.
Methods: Overall, 161 fecal samples were collected from healthy children and patients with FAP or CeD in Hitit University Erol Olçok Research and Training Hospital, Corum, Turkey between 2016-2018. Samples were examined using both native-Lugol (NL) and trichrome-stained (TS) smears, and further analyses by PCR and Sanger sequencing were performed. A standard questionnaire was applied to obtain demographic, socioeconomic, epidemiological data.
Results: Blastocystis was found in 10.6% of the total study population. Neither bacteria nor any other parasites were found, except for one Giardia (0.6%) in the CeD group. The presence/absence of the parasite was not found to be associated with demographic, socioeconomic and epidemiological factors. Blastocysis was detected in 11.5% (6/52) of the CeD, 7.7% (4/52) of the FAP, and 12.3% (7/57) of the healthy group. Diagnostic methods were similar in terms of Blastocystis detection (P= 0.671), and there was fair agreement between the NL, TS and PCR (Fleiss' Kappa=0.847, P=0.001). ST2 (42.8%) and ST3 (35.7%) were the predominant STs followed by ST1 (21.4%).
Conclusion: We observed no difference between study groups in terms of Blastocystis prevalence. ST1, ST2 and ST3 subtypes were detected. Blastocystis prevalence and STs were not related to any of the demographic, socioeconomic and epidemiological factors.
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11. Seyer A, Karasartova D, Ruh E, et al. Is "dried stool spots on filter paper method (DSSFP)" more sensitive and effective for detecting Blastocystis spp. and their subtypes by PCR and sequencing? Parasitol Res. 2016;115(12):4449-55.
12. Scicluna SM, Tawari B, Clark CG. DNA barcoding of blastocystis. Protist. 2006;157(1):77-85.
13. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987;4(4):406-25.
14. Anonymus. Avaialble from: http://entamoeba.lshtm.ac.uk/ref.blasto.txt
15. Guandalini S, Setty M. Celiac Disease.Curr Opin Gastroenterol. 2008;24(6):707-12.
16. Pecora F, Persico F, Gismondi P, et al. Gut Microbiota in Celiac Disease: Is There Any Role for Probiotics?. Front Immunol. 2020;11:957. Published 2020 May 15. doi:10.3389/fimmu.2020.00957.
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18. Petersen AM, Stensvold CR, Mirsepasi H, et al. Active ulcerative colitis associated with low prevalence of Blastocystis and Dientamoeba fragilis infection. Scand J Gastroenterol. 2013;48(5):638-9.
19. Rossen NG, Bart A, Verhaar N, et al. Low prevalence of Blastocystis sp. in active ulcerative colitis patients. Eur J Clin Microbiol Infect Dis. 2015;34(5):1039-44.
20. Beghini F, Pasolli E, Truong TD, et al. Large-scale comparative metagenomics of Blastocystis, a common member of the human gut microbiome. ISME J. 2017;11(12):2848-63.
21. LaBeaud AD, Nayakwadi Singer M, McKibben M, et al. Parasitism in children aged three years and under: Relationship between infection and growth in rural coastal Kenya. PLoS Negl Trop Dis. 2015;9(5):e0003721.
22. Degerli S, Celiksoz A, Aslan A, et al. Comparison of the results of examination of fecal samples from students at six months intervals in the Alahaci village primary school in Sivas. Turkiye Parazitol Derg. 2006;30(4):305-7.
23. Dogan N, Aydin M, Tuzemen NU, et al. Subtype distribution of Blastocystis spp. isolated from children in Eskisehir, Turkey. Parasitol Int. 2017;66(1):948-51.
24. Cabrine-Santos M, Cintra Edo N, do Carmo RA,et al. Occurrence of Blastocystis spp. in uberaba, minas gerais, Brazil. Rev Inst Med Trop Sao Paulo. 2015;57(3):211-4.
25. Forsell J, Granlund M, Samuelsson L, et al. High occurrence of Blastocystis sp. subtypes 1-3 and Giardia intestinalis assemblage B among patients in Zanzibar, Tanzania. Parasit Vectors. 2016;9(1):370.
26. Beyhan YE, Yilmaz H, Cengiz ZT, et al. Clinical significance and prevalence of Blastocystis hominis in Van, Turkey. Saudi Med J. 2015;36(9):1118-21.
27. Cociancic P, Torrusio SE, Zonta ML, et al. Risk factors for intestinal parasitoses among children and youth of Buenos Aires, Argentina. One Health. 2019;9:100116.
28. Oliveira-Arbex AP, David EB, Guimaraes S. Blastocystis genetic diversity among children of low-income daycare center in Southeastern Brazil. Infect Genet Evol. 2018;57:59-63.
29. Yoshikawa H, Tokoro M, Nagamoto T, et al. Molecular survey of Blastocystis sp. from humans and associated animals in an Indonesian community with poor hygiene. Parasitol Int. 2016;65(6 Pt B):780-4.
30. Basak S, Rajurkar MN, Mallick SK. Detection of Blastocystis hominis: a controversial human pathogen. Parasitol Res. 2014;113(1):261-5.
31. Parkar U, Traub RJ, Vitali S, et al. Molecular characterization of Blastocystis isolates from zoo animals and their animal-keepers. Vet Parasitol. 2010;169(1-2):8-17.
32. Rajah Salim H, Suresh Kumar G, Vellayan S, et al. Blastocystis in animal handlers. Parasitol Res. 1999;85(12):1032-3.
33. Assavapongpaiboon B, Bunkasem U, Sanprasert V, et al. A Cross-Sectional Study on Intestinal Parasitic Infections in Children in Suburban Public Primary Schools, Saraburi, the Central Region of Thailand. Am J Trop Med Hyg. 2018;98(3):763-7.
34. Stensvold CR, Clark CG. Molecular identification and subtype analysis of Blastocystis. Curr Protoc Microbiol. 2016;43:20A. 2. 1-20A. 2 .10.
35. Dogruman-Al F, Dagci H, Yoshikawa H, et al. A possible link between subtype 2 and asymptomatic infections of Blastocystis hominis. Parasitol Res. 2008;103(3):685-9.
36. Ertug S, Malatyali E, Ertabaklar H, et al. Subtype distribution of Blastocystis isolates and evaluation of clinical symptoms detected in Aydin province, Turkey. Mikrobiyol Bul. 2015;49(1):98-104.
37. Yersal O, Malatyali E, Ertabaklar H, et al. Blastocystis subtypes in cancer patients: Analysis of possible risk factors and clinical characteristics. Parasitol Int. 2016;65(6 Pt B):792-6.
2. Rosinach M, Esteve M, Gonzalez C, et al. Lymphocytic duodenosis: aetiology and long-term response to specific treatment. Dig Liver Dis. 2012;44(8):643-8.
3. Tan KS. New insights on classification, identification, and clinical relevance of Blastocystis spp. Clin Microbiol Rev. 2008;21(4):639-65.
4. Sagnuankiat S, Wanichsuwan M, Bhunnachet E, et al. Health status of immigrant children and environmental survey of child daycare centers in Samut Sakhon Province, Thailand. J Immigr Minor Health. 2016;18(1):21-7.
5. Rebolla MF, Silva EM, Gomes JF, et al. High prevalence of Blastocystis spp. infection in children and staff members attending public urban schools in Sao Paulo State, Brazil. Rev Inst Med Trop Sao Paulo. 2016;58:31.
6. Ozcakir O, Gureser S, Erguven S, et al. Characteristics of Blastocystis hominis infection in a Turkish university hospital. Turkiye Parazitol Derg. 2007;31(4):277-82.
7. Scanlan PD. Blastocystis: past pitfalls and future perspectives. Trends Parasitol. 2012;28(8):327-34.
8. Dogruman-Al F, Yoshikawa H, Kustimur S, et al. PCR-based subtyping of Blastocystis isolates from symptomatic and asymptomatic individuals in a major hospital in Ankara, Turkey. Parasitol Res. 2009;106(1):263-8.
9. Comba A, Güreser AS, Karasartova D, et al. Thiol-disulfide homeostasis in children with celiac disease. Pediatr Int. 2020 Aug;62(8):950-956. doi: 10.1111/ped.14243. PMID: 32239752.
10. Seyer A, Karasartova D, Ruh E, et al. Epidemiology and prevalence of Blastocystis spp. in North Cyprus. Am J Trop Med Hyg. 2017;96(5):1164-70.
11. Seyer A, Karasartova D, Ruh E, et al. Is "dried stool spots on filter paper method (DSSFP)" more sensitive and effective for detecting Blastocystis spp. and their subtypes by PCR and sequencing? Parasitol Res. 2016;115(12):4449-55.
12. Scicluna SM, Tawari B, Clark CG. DNA barcoding of blastocystis. Protist. 2006;157(1):77-85.
13. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987;4(4):406-25.
14. Anonymus. Avaialble from: http://entamoeba.lshtm.ac.uk/ref.blasto.txt
15. Guandalini S, Setty M. Celiac Disease.Curr Opin Gastroenterol. 2008;24(6):707-12.
16. Pecora F, Persico F, Gismondi P, et al. Gut Microbiota in Celiac Disease: Is There Any Role for Probiotics?. Front Immunol. 2020;11:957. Published 2020 May 15. doi:10.3389/fimmu.2020.00957.
17. Chibbar R, Dieleman LA. The Gut Microbiota in Celiac Disease and probiotics. Nutrients.2019;11(10):2375. Published 2019 Oct 5. doi:10.3390/nu11102375 .
18. Petersen AM, Stensvold CR, Mirsepasi H, et al. Active ulcerative colitis associated with low prevalence of Blastocystis and Dientamoeba fragilis infection. Scand J Gastroenterol. 2013;48(5):638-9.
19. Rossen NG, Bart A, Verhaar N, et al. Low prevalence of Blastocystis sp. in active ulcerative colitis patients. Eur J Clin Microbiol Infect Dis. 2015;34(5):1039-44.
20. Beghini F, Pasolli E, Truong TD, et al. Large-scale comparative metagenomics of Blastocystis, a common member of the human gut microbiome. ISME J. 2017;11(12):2848-63.
21. LaBeaud AD, Nayakwadi Singer M, McKibben M, et al. Parasitism in children aged three years and under: Relationship between infection and growth in rural coastal Kenya. PLoS Negl Trop Dis. 2015;9(5):e0003721.
22. Degerli S, Celiksoz A, Aslan A, et al. Comparison of the results of examination of fecal samples from students at six months intervals in the Alahaci village primary school in Sivas. Turkiye Parazitol Derg. 2006;30(4):305-7.
23. Dogan N, Aydin M, Tuzemen NU, et al. Subtype distribution of Blastocystis spp. isolated from children in Eskisehir, Turkey. Parasitol Int. 2017;66(1):948-51.
24. Cabrine-Santos M, Cintra Edo N, do Carmo RA,et al. Occurrence of Blastocystis spp. in uberaba, minas gerais, Brazil. Rev Inst Med Trop Sao Paulo. 2015;57(3):211-4.
25. Forsell J, Granlund M, Samuelsson L, et al. High occurrence of Blastocystis sp. subtypes 1-3 and Giardia intestinalis assemblage B among patients in Zanzibar, Tanzania. Parasit Vectors. 2016;9(1):370.
26. Beyhan YE, Yilmaz H, Cengiz ZT, et al. Clinical significance and prevalence of Blastocystis hominis in Van, Turkey. Saudi Med J. 2015;36(9):1118-21.
27. Cociancic P, Torrusio SE, Zonta ML, et al. Risk factors for intestinal parasitoses among children and youth of Buenos Aires, Argentina. One Health. 2019;9:100116.
28. Oliveira-Arbex AP, David EB, Guimaraes S. Blastocystis genetic diversity among children of low-income daycare center in Southeastern Brazil. Infect Genet Evol. 2018;57:59-63.
29. Yoshikawa H, Tokoro M, Nagamoto T, et al. Molecular survey of Blastocystis sp. from humans and associated animals in an Indonesian community with poor hygiene. Parasitol Int. 2016;65(6 Pt B):780-4.
30. Basak S, Rajurkar MN, Mallick SK. Detection of Blastocystis hominis: a controversial human pathogen. Parasitol Res. 2014;113(1):261-5.
31. Parkar U, Traub RJ, Vitali S, et al. Molecular characterization of Blastocystis isolates from zoo animals and their animal-keepers. Vet Parasitol. 2010;169(1-2):8-17.
32. Rajah Salim H, Suresh Kumar G, Vellayan S, et al. Blastocystis in animal handlers. Parasitol Res. 1999;85(12):1032-3.
33. Assavapongpaiboon B, Bunkasem U, Sanprasert V, et al. A Cross-Sectional Study on Intestinal Parasitic Infections in Children in Suburban Public Primary Schools, Saraburi, the Central Region of Thailand. Am J Trop Med Hyg. 2018;98(3):763-7.
34. Stensvold CR, Clark CG. Molecular identification and subtype analysis of Blastocystis. Curr Protoc Microbiol. 2016;43:20A. 2. 1-20A. 2 .10.
35. Dogruman-Al F, Dagci H, Yoshikawa H, et al. A possible link between subtype 2 and asymptomatic infections of Blastocystis hominis. Parasitol Res. 2008;103(3):685-9.
36. Ertug S, Malatyali E, Ertabaklar H, et al. Subtype distribution of Blastocystis isolates and evaluation of clinical symptoms detected in Aydin province, Turkey. Mikrobiyol Bul. 2015;49(1):98-104.
37. Yersal O, Malatyali E, Ertabaklar H, et al. Blastocystis subtypes in cancer patients: Analysis of possible risk factors and clinical characteristics. Parasitol Int. 2016;65(6 Pt B):792-6.
| Files | ||
| Issue | Vol 17 No 3 (2022) | |
| Section | Original Article(s) | |
| DOI | https://doi.org/10.18502/ijpa.v17i3.10619 | |
| Keywords | ||
| Blastocystis Celiac disease Functional abdominal pain Children Gastroenterology | ||
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How to Cite
1.
Gureser A, Comba A, Karasartova D, Koşar N, Keskin A, Stensvold C, Taylan-Ozkan A. Detection of Blastocystis Subtypes in Children with Functional Abdominal Pain and Celiac Disease in Çorum, Turkey. Iran J Parasitol. 2022;17(3):296-305.
