Seroepidemiological Study of Visceral Leishmaniasis (Kala-azar) in Children under 12 Years Old in North of Iran: An Observational Study in 2019-2020
Background: Visceral leishmaniasis (VL) caused by the Leishmania donovani complex that is transmitted by the bites of female sandflies. Mediterranean type of VL caused by L. infantum. While, Roudbar County of Guilan Province has been introduced as a suspected cutaneous leishmaniasis focus; there are no published data on the seroprevalence of VL in Guilan Province. We aimed to investigate the seroprevalence of this disease in Roudbar County.
Methods: This descriptive cross-sectional study was carried out in 2019-2020 among children less than 12 years of age to determine the seroprevalence of VL by direct agglutination test (DAT). Blood samples were randomly collected from 918 children under 12 years of age refers to the public health center in the clusters.
Results: Out of 918 children, 14 (1.52%) showed anti-Leishmania antibodies, with 4 (0.43%), 2 (0.21%), 8 (0.87%) anti-L. infantum antibodies at titers 1:800, 1:1600 and ≥1: 3200 respectively. All children with anti-Leishmania antibody titers of ≥1:800 were evaluated by a physician. Clinical manifestation of VL including fever, anemia and hepatosplenomegaly observed in a 6-year-old boy from Defraz village with anti-Leishmania antibody of titers ≥102400.This patient was admitted to the pediatric hospital in Rasht, capital of Guilan province, Iran and was successfully treated.
Conclusion: VL is being circulated with low prevalence in children up to 12 years old in Roudbar, northern part of Iran. Accordingly, it is critical to improve the awareness of physicians and public health supervisors about the importance of this fatal disease in Guilan province and especially in Roudbar area.
2. Pavli A, Maltezou HC. Leishmaniasis, an emerging infection in travelers. Int J Infect Dis. 2010;14(12):e1032-9.
3. Nadim A, Navid Hamidi A, Javadian E, Bidruni GT, Amini H. Present status of Kala-azar in Iran. Am J Trop Med Hyg. 1978;27:25-8.
4. Mohebali M. Visceral leishmaniasis in Iran: Review of the Epidemiological and Clinical Features. Iran J Parasitol. 2013;8(3):348-58.
5. Mohebali M, Hajjaran H, Hamzavi Y, et al. Epidemiological aspects of canine visceral leishmaniosis in the Islamic Republic of Iran. Vet Parasitol. 2005;129(3-4):243-51.
6. Nadim A, Javadian E, ZamenMomeni A, Mohebali M. Leishmania parasite and leishmaniasis: Tehran: University Publishing Center,; 2008.
7. Shirzadi MR, Esfahania SB, Mohebalia M, et al. Epidemiological status of leishmaniasis in the Islamic Republic of Iran, 1983-2012. East Mediterr Health J. 2015;21(10):736-42.
8. Mohebali M, Keshavarz H, Shirmohammad S, et al. The diagnostic accuracy of direct agglutination test for serodiagnosis of human visceral leishmaniasis: a systematic review with meta-analysis. BMC Infect Dis. 2020;20(1):946.
9. Sarkari B, Rezaei Z, Mohebali M. Immunodiagnosis of Visceral Leishmaniasis: Current Status and Challenges: A Review Article. Iran J Parasitol. 2018;13(3):331-41.
10. Norouzi B, Hanafi-Bojd AA, Moin-Vaziri V, Noorallahi A, Azari-Hamidian S. An Inventory of the Sand Flies (Diptera: Psychodidae) of Rudbar County, a New Focus of Leishmaniasis in Northern Iran, with a Taxonomic Note on the Subgenus Larroussius. J Arthropod Borne Dis. 2020;14(3):302-16.
11. Iran data portal. Census of the Islamic Republic of Iran. https://irandataportal.syr.edu/census
12. Heidari A, Mohebali M, Kabir K, et al. Visceral Leishmaniasis in Rural Areas of Alborz Province of Iran and Implication to Health Policy. Korean J Parasitol. 2015;53(4):379-83.
13. Mahami M, Mohebali M, Keshavarz H, Hajaran H, Akhoondi B, ZZea. A seroepidemiological survey of visceral leishmaniasis (KALA-AZAR) in Germi district, Ardabil Province. SJSPH. 2006;4(1):45-55.
14. Mahmoudvand H, Mohebali M, Sharifi I, et al. Epidemiological aspects of visceral leishmaniasis in baft district, kerman province, southeast of Iran. Iran J Parasitol 2011;6(1):1-11.
15. Torabi V, Mohebali M, Edrissian G, et al. Seroepidemiological Survey of Visceral Leishmaniasis by Direct Agglutination Test in Bojnoord District, North Khorasan Province in 2007. Iran J Epidmiol. 2009;4(3 and 4):43-50 (In Persian).
16. Hamzavi Y, Hamzeh B, Mohebali M, et al. Human visceral leishmaniasis in Kermanshah Province, Western iran, during 2011-2012. Iran J Parasitol. 2012;7(4):49-56.
17. Badaró R, Jones TC, Lorenço R, et al. A prospective study of visceral leishmaniasis in an endemic area of Brazil. J Infect Dis. 1986;154(4):639-49.
18. Gidwani K, Kumar R, Rai M, Sundar S. Longitudinal seroepidemiologic study of visceral leishmaniasis in hyperendemic regions of Bihar, India. Am J Trop Med Hyg. 2009;80(3):345-6.
19. Adel A, Boughoufalah A, Saegerman C, et al. Epidemiology of visceral leishmaniasis in Algeria: an update. PLoS One. 2014;9(6):e99207.
20. Mohebali M, Edrissian GH, Shirzadi MR, et al. An observational study on the current distribution of visceral leishmaniasis in different geographical zones of Iran and implication to health policy. Travel Med Infect Dis. 2011;9(2):67-74.
21. Tanir G, Taylan Ozkan A, Dağlar E. Pediatric visceral Leishmaniasis in Turkey. Pediatr Int. 2006;48(1):66-9.
22. Patil RR, Muliyil JP, Nandy A, Addy A, Maji AK, Chatterjee P. Dynamics of the antibodies in cohorts of cured cases of visceral leishmaniasis: its implication on the validity of serological test, value in prognosis and in post therapeutic assessment. Hum Vaccin Immunother. 2012;8(6):725-30.
23. Chegeni Sharafy A, Ormazdi H, Mohebali M, Akhlaghi L, Sharafy M M, Akhondi B. Seroepidemiological study of visceral Leishmaniasis (human infection) in East Myankooh area, in Lorestan Province by Direct Agglutination Test (DAT). Yafteh Journal. 2005;7(3):31-5.
24. Sarkari B, Pedram N, Mohebali M, et al. Seroepidemiological study of visceral leishmaniasis in Booyerahmad district, south-west Islamic Republic of Iran. East Mediterr Health J. 2010;16(11):1133-6.
25. Rey LC, Martins CV, Ribeiro HB, Lima AA. American visceral leishmaniasis (kala-azar) in hospitalized children from an endemic area. J Pediatr (Rio J) 2005;81(1):73-8.
26. Sharma MC, Gupta AK, Saran R, Sinha SP. The effect of age and sex on incidence of kala-azar. J Commun Dis. 1990;22(4):277-8.
27. Rêgo FD, Rugani JM, Shimabukuro PH, Tonelli GB, Quaresma PF, Gontijo CM. Molecular detection of Leishmania in phlebotomine sand flies (Diptera: Psychodidae) from a coetaneous leishmaniasis focus atXakriabá Indigenous Reserve, Brazil. PLoS One. 2015;10(4):e0122038.
|Issue||Vol 17 No 3 (2022)|
|Visceral leishmaniasis Seroepidemiology Direct agglutination test Children Iran|
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