Investigation of Neospora caninum Seroprevalence and Asso-ciation with Reproductive Problems in Cows in Burdur Province of Turkey
,
Abstract
Background: An apicomplexan protozoon Neospora caninum, causative agent of neosporosis, is recognized as one of the most common and important cause of sporadic and endemic bovine abortion and reduced reproductivity in dairy and beef cattle worldwide. The present study was conducted to investigate the relationship between N. caninum seroprevalence and infertility problems in 400 cows in Burdur city, Turkey.
Methods: Blood samples were collected from vena jugularis into sterile serum tubes from 49 aborted, 58 infertil, 48 pregnant and 245 healthy cows for the findings of reproductive anamnesis during a period of March 2010 to March 2011. Sera samples were analyzed by competitive ELISA kit.
Results: The seroprevalences were 7.7%, 6.4% and 4.2% in 2-4, ≤2 and ≥4 age groups respectively and no statistically significance observed between age groups. Seropositivity rates were 5.7%, 5.1%, 4.5%, 3.6% in Holstein, Montofon, cross-breeds and Simental breeds respectively. Seroprevalence differences was not statistically significant among cattle breeds. Antibodies to N. caninum were found in rates of 16.3%, 6.9%, 6.3%, 2.4% in aborted, infertile, pregnant and healthy cows respectively and there was a significant difference (P<0.01) between aborted and healthy animals. Seroprevalences were Yeşilova 10%, Gölhisar and Ağlasun 8%, Bucak, Çavdır and Kemer 4%, Karamanlı and Burdur Centrum 2%, according to districts.
Conclusion: The seroprevalence of N. caninum was revealed in Burdur region. It was emphasized that N. caninum infection should not be ignored in reproductive problems, especially in abortion cases.
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8. Ortega-Mora LM, Fernandez-Garcia A, Gomez-Bautista M. Diagnosis of bovine neosporosis: Recent advances and per-spectives. Acta Parasitol. 2006; 51(1):1-14.
9. Alvarez-Garcia G, Garcia-Culebras A, Gutierrez-Exposito D, et al. Serological di-agnosis of bovine neosporosis: a compar-ative study of commercially available ELI-SA tests. Vet Parasitol.2013; 198(1-2):85-95.
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11. Gondim LFP, Sartor IF, Hasegawa M. Seroprevalance of Neospora caninum in dairy cattle in Bahia, Brazil. Vet Parasitol.1999; 86(1):71-5.
12. Atkinson R, Harper PAW, Reichel MP, et al. Progress in the serodiagnosis of Neospo-ra caninum infections of cattle. Parasitol To-day. 2000; 16(3):110-4.
13. Baszler TV, Adams S, Schalie JV, et al. Val-idation of a commercially available mono-clonal antibody-based competitive-inhibition enzyme-linked immunosorbent assay for detection of serum antibodies to Neospora caninum in cattle. J Clin Microbiol. 2001; 39(11):3851-7.
14. Haddad JP, Dohoo IR, VanLeewen JA. A review of Neospora caninum in dairy and beef cattle-a Canadian perspective. Can Vet J. 2005; 46(3):230-43.
15. Quintanilla-Gozalo A, Pereira-Bueno J, Tabares E, et al. Seroprevalence of Neospo-ra caninum infection in dairy and beef cattle in Spain. Int J Parasitol, 1999; 29(8):1201-8.
16. Vazquez ZG, Vazquez CC, Espinosa LM, et al. Serological survey of Neospora caninum infection in dairy cattle herds in Aquasca-lientes, Mexica. Vet Parasitol.2002; 106(2):115-20.
17. Gonzalez JJG, Cruz-Vazquez C, Esparza LM, et al. Management factors associated with seroprevalence to Neospora caninum in-fection in dairy cattle in Aguascalientes, Mexico. Vet Mexico.2007; 38:261-70.
18. Canada N, Carvalheira J, Meireles CS, et al. Prevalence of Neospora caninum infection in dairy cows and its consequences for re-productive management. Theriogenology. 2004; 62(7):1229-35.
19. Koiwai M, Hamaoka T, Haritani M, et al. Nationwide seroprevalence of Neospora caninum among dairy cattle in Japan. Vet Parasitol. 2006; 135(2):175-9.
20. Sotiraki S, Brozos C, Samartzi F, et al. Ne-ospora caninum infection in Greek dairy cattle herds detected by two antibody assays in individual milk samples. Vet Parasitol.2008; 152(1-2):79-84.
21. Mitrea IL, Enachescu V, Radulescu R, et al. Seroprevalence of Neospora caninum infec-tion on dairy cattle in farms from southern Romania. J Parasitol.2012; 98(1):69-72.
22. Padilha MAC, Wasen G, Souza AP, et al. Neospora caninum: Seroprevalence in beef cattle in the mountainous region of Santa Catarina, Brazil. Semin Cienc Agrar.2017; 38(1):273-82.
23. Norollahi-Fard SR, Khalili M, Fazli O, et al. Seroprevalence of Neospora caninum in cattle of Neishabour, Northeast Iran. Slov Vet Res.2017; 54(1): 5-9.
24. Noori M, Rasekh M, Ganjali M, et al. Se-roprevalence of Neospora caninum infection and associated risk factors in cattle of Sis-tan areas, Southeastern Iran in 2016. Iran J Parasitol.2019; 14(2): 340-6.
25. Cedeno QD, Benavides BB. Seropreva-lence and risk factors associated to Neospo-ra caninum in dairy cattle herds in the mu-nicipality of Pasto, Colombia. Rev MVZ Cordoba.2013; 18(1):3311-6.
26. Llano HAB, Guimaraes MS, Soares RM, et al. Seroprevalence and risk factors for Ne-ospora caninum infection in cattle from the eastern Antioquia, Colombia. Vet Anim Sci. 2018; 6:69-74.DOI: 10.1016/j.vas.2018.03.001
27. Semango G, Hamilton CM, Kreppel K, et al. The Sero-epidemiology of Neospora cani-num in Cattle in Northern Tanzania. Front Vet Sci.2019; 6:327.
28. Oncel T, Biyikoglu G. Neosporosis in dairy cattle in Sakarya, Turkey. J Res Vet Med. 2003; 22:87-9.
29. Biyikoglu G, Bagci O, Oncel T.Serological Survey of Neospora caninum Infection. Indi-an Vet J.2005; 82(3):345-6.
30. Sevgili M, Altas MG, Keskin O. Sero-prevalence of Neospora caninum in cattle in the province of Sanliurfa. Turkish J Vet Anim Sci, 2005; 29:127-30.
31. Ica A, Yildirim A, Duzlu O, et al. Sero-prevalence of Neospora caninum in Cattle in the Region of Kayseri. Türkiye Parasitol Derg.2006; 30(2):92-4.
32. Vural G, Aksoy E, Bozkir M, et al. Sero-prevalence of Neospora caninum in dairy cat-tle herds in Central Anatolia, Turkey. Vet Arh.2006; 76:343-9.
33. Aktas M, Saki CE, Altay K, et al. Survey of Neospora caninum in cattle in some provinces in the Eastern Anatolian region. Turkiye Parasitol Derg.2005; 29(1):22-5.
34. Simsek S, Utuk AE, Koroglu E, et al. Se-roprevalence of Neospora caninum in repeat breeder dairy cows in Turkey. Arch Tierz.2008; 51(2):143-8.DOI: 10.5194/aab-51-143-2008
35. Alan M, Cetin Y, Sendag S, et al. Seroprev-alence of antibodies against Neospora cani-num in cows in Van province. Kafkas Univ Vet Fak Derg.2011; 17(5):767-71.
36. Akca A, Gokce HI, Guy CS, et al. Preva-lence of antibodies to Neospora caninum in local and imported cattle breeds in the Kars province of Turkey. Res Vet Sci. 2005; 78(2):123-6.DOI: 10.1016/j.rvsc.2004.08.006
37. Mor N, Akca A. Kars Yöresinde sığır ve köpeklerde Neospora caninum üzerine epi-demiyolojik araştırmalar: Gruplararası çalışma. Kafkas Univ Vet Fak Derg. 2012; 18(Suppl-A), A193-9.DOI: 10.9775/kvfd.2012.6181
38. Kul O, Kabakci N, Yildiz K, et al. Neospora caninum associated with epidemic abortions in dairy cattle: the first clinical neosporosis report in Turkey. Vet Parasitol.2009; 159(1):69-72.DOI: 10.1016/j.vetpar. 2008.10.019
39. Balkaya I, Bastem Z, Avcioglu H, et al. Se-roprevalence of Neospora caninum Antibod-ies in Cattle in Eastern Turkey. Isr J Vet Med.2009; 67(2):109-12.
40. Celik HA, Kozan E, Eser M, et al. A re-search on seroprevalence of Neospora cani-num in cattle. Ankara Univ Vet Fak Derg, 2013; 60:99-102.DOI: 10.1501/Vetfak_0000002561
41. Karatepe B, Karatepe M. Seroprevalence of Neospora caninum in cattle in Nigde Prov-ince, Turkey. Isr J Vet Med.2016; 71:39-42.
42. Yildiz K, Gokpinar S, Sursal N, et al. Sero-prevalence of Neospora caninum in Dairy Cattle Raised in Cicekdagi District of Kirsehir Province. Turkiye Parasitol Derg.2017; 41(3):135-8.DOI: 10.5152/tpd.2017.5218
43. Eski F, Utuk AE. Detection of Anti-Neospora caninum Antibodies in Cattle in Adana Province of Turkey. Van Vet J. 2018; 29(2):93-9.
44. Kasap S, Ertunc S, Temizel EM, et al. A study of Neospora caninum antibody sero-prevalence in dairy cows in Turkey. J Hel-lenıc Vet Med Soc, 2020; 71:2019-22.DOI: 10.12681/jhvms.22950
45. Wouda W, Moen AR, Schukken YH. Abortion risk in progeny of cows after a Neospora caninum epidemic. Theriogenolo-gy.1998; 49(7):1311-6.DOI: 10.1016/S0093-691X(98)00078-8
46. Thurmond MC, Hietala SK. Culling asso-ciated with Neospora caninum in dairy cows. Am J Vet Res.1996; 57:1559-62.
47. Sanderson MW, Gay JM, Baszler TV. Ne-ospora caninum seroprevalence and associat-ed risk factors in beef cattle in the North-western United States. Vet Parasitol.2000; 90:15-24.DOI: 10.1016/s0304-4017(00)00234-x
48. Sadrebazzaz A, Haddadzadeh H, Esmailnia K, et al. Serological prevalence of Neospora caninum in healthy and aborted dairy cattle in Mashhad, Iran. Vet Parasitol. 2004; 124: 201-4. DOI: 10.1016/j.vetpar.2004.06.027
49. Dumanli N, Aktas M. Toxoplasmatidae (Toxoplasma, Neospora). In: Dumanli N, Karaer Z, editors. Veteriner Protozooloji. Ankara; Medisan:2010. p. 128-35.
2. Thilsted JP, Dubey JP. Neosporosis-like abortions in a herd of dairy cattle. J Vet Diag Invest.1989; 1(3):205-209.
3. Barr BC, Anderson ML, Blanchard PC, et al. Bovine fetal encephalitis and myocardi-tis associated with protozoal infections. Vet Pathol. 1990; 27(5):354-61.
4. Dubey JP, Schares G. Neosporosis in an-imals-The last five years. Vet Parasi-tol.2011; 180(1-2):90-108.DOI: 10.1016/j.vetpar.2011.05.031
5. Anderson ML, Andrianarivo AG, Conrad PA. Neosporosis in cattle. Anim Reprod Sci. 2000; 60-61:417-31.
6. Dubey JP, Schares G, Ortega-Mora LM. Epidemiology and Control of Neosporo-sis and Neospora caninum. Clin Microbiol Rev.2007; 20(2):323-67.
7. Lopez‐Gatius F, Lopez‐Bejar M, Muru-gavel K, et al. Neospora‐associated Abortion Episode over a 1‐Year Period in a Dairy Herd in North‐east Spain. J Vet Med B In-fect Dis Vet Public Health. 2004; 51(7):348-52.
8. Ortega-Mora LM, Fernandez-Garcia A, Gomez-Bautista M. Diagnosis of bovine neosporosis: Recent advances and per-spectives. Acta Parasitol. 2006; 51(1):1-14.
9. Alvarez-Garcia G, Garcia-Culebras A, Gutierrez-Exposito D, et al. Serological di-agnosis of bovine neosporosis: a compar-ative study of commercially available ELI-SA tests. Vet Parasitol.2013; 198(1-2):85-95.
10. Dubey JP, Schares G. Diagnosis of bovine neosporosis. Vet Parasitol. 2006; 140(1-2):1-34.
11. Gondim LFP, Sartor IF, Hasegawa M. Seroprevalance of Neospora caninum in dairy cattle in Bahia, Brazil. Vet Parasitol.1999; 86(1):71-5.
12. Atkinson R, Harper PAW, Reichel MP, et al. Progress in the serodiagnosis of Neospo-ra caninum infections of cattle. Parasitol To-day. 2000; 16(3):110-4.
13. Baszler TV, Adams S, Schalie JV, et al. Val-idation of a commercially available mono-clonal antibody-based competitive-inhibition enzyme-linked immunosorbent assay for detection of serum antibodies to Neospora caninum in cattle. J Clin Microbiol. 2001; 39(11):3851-7.
14. Haddad JP, Dohoo IR, VanLeewen JA. A review of Neospora caninum in dairy and beef cattle-a Canadian perspective. Can Vet J. 2005; 46(3):230-43.
15. Quintanilla-Gozalo A, Pereira-Bueno J, Tabares E, et al. Seroprevalence of Neospo-ra caninum infection in dairy and beef cattle in Spain. Int J Parasitol, 1999; 29(8):1201-8.
16. Vazquez ZG, Vazquez CC, Espinosa LM, et al. Serological survey of Neospora caninum infection in dairy cattle herds in Aquasca-lientes, Mexica. Vet Parasitol.2002; 106(2):115-20.
17. Gonzalez JJG, Cruz-Vazquez C, Esparza LM, et al. Management factors associated with seroprevalence to Neospora caninum in-fection in dairy cattle in Aguascalientes, Mexico. Vet Mexico.2007; 38:261-70.
18. Canada N, Carvalheira J, Meireles CS, et al. Prevalence of Neospora caninum infection in dairy cows and its consequences for re-productive management. Theriogenology. 2004; 62(7):1229-35.
19. Koiwai M, Hamaoka T, Haritani M, et al. Nationwide seroprevalence of Neospora caninum among dairy cattle in Japan. Vet Parasitol. 2006; 135(2):175-9.
20. Sotiraki S, Brozos C, Samartzi F, et al. Ne-ospora caninum infection in Greek dairy cattle herds detected by two antibody assays in individual milk samples. Vet Parasitol.2008; 152(1-2):79-84.
21. Mitrea IL, Enachescu V, Radulescu R, et al. Seroprevalence of Neospora caninum infec-tion on dairy cattle in farms from southern Romania. J Parasitol.2012; 98(1):69-72.
22. Padilha MAC, Wasen G, Souza AP, et al. Neospora caninum: Seroprevalence in beef cattle in the mountainous region of Santa Catarina, Brazil. Semin Cienc Agrar.2017; 38(1):273-82.
23. Norollahi-Fard SR, Khalili M, Fazli O, et al. Seroprevalence of Neospora caninum in cattle of Neishabour, Northeast Iran. Slov Vet Res.2017; 54(1): 5-9.
24. Noori M, Rasekh M, Ganjali M, et al. Se-roprevalence of Neospora caninum infection and associated risk factors in cattle of Sis-tan areas, Southeastern Iran in 2016. Iran J Parasitol.2019; 14(2): 340-6.
25. Cedeno QD, Benavides BB. Seropreva-lence and risk factors associated to Neospo-ra caninum in dairy cattle herds in the mu-nicipality of Pasto, Colombia. Rev MVZ Cordoba.2013; 18(1):3311-6.
26. Llano HAB, Guimaraes MS, Soares RM, et al. Seroprevalence and risk factors for Ne-ospora caninum infection in cattle from the eastern Antioquia, Colombia. Vet Anim Sci. 2018; 6:69-74.DOI: 10.1016/j.vas.2018.03.001
27. Semango G, Hamilton CM, Kreppel K, et al. The Sero-epidemiology of Neospora cani-num in Cattle in Northern Tanzania. Front Vet Sci.2019; 6:327.
28. Oncel T, Biyikoglu G. Neosporosis in dairy cattle in Sakarya, Turkey. J Res Vet Med. 2003; 22:87-9.
29. Biyikoglu G, Bagci O, Oncel T.Serological Survey of Neospora caninum Infection. Indi-an Vet J.2005; 82(3):345-6.
30. Sevgili M, Altas MG, Keskin O. Sero-prevalence of Neospora caninum in cattle in the province of Sanliurfa. Turkish J Vet Anim Sci, 2005; 29:127-30.
31. Ica A, Yildirim A, Duzlu O, et al. Sero-prevalence of Neospora caninum in Cattle in the Region of Kayseri. Türkiye Parasitol Derg.2006; 30(2):92-4.
32. Vural G, Aksoy E, Bozkir M, et al. Sero-prevalence of Neospora caninum in dairy cat-tle herds in Central Anatolia, Turkey. Vet Arh.2006; 76:343-9.
33. Aktas M, Saki CE, Altay K, et al. Survey of Neospora caninum in cattle in some provinces in the Eastern Anatolian region. Turkiye Parasitol Derg.2005; 29(1):22-5.
34. Simsek S, Utuk AE, Koroglu E, et al. Se-roprevalence of Neospora caninum in repeat breeder dairy cows in Turkey. Arch Tierz.2008; 51(2):143-8.DOI: 10.5194/aab-51-143-2008
35. Alan M, Cetin Y, Sendag S, et al. Seroprev-alence of antibodies against Neospora cani-num in cows in Van province. Kafkas Univ Vet Fak Derg.2011; 17(5):767-71.
36. Akca A, Gokce HI, Guy CS, et al. Preva-lence of antibodies to Neospora caninum in local and imported cattle breeds in the Kars province of Turkey. Res Vet Sci. 2005; 78(2):123-6.DOI: 10.1016/j.rvsc.2004.08.006
37. Mor N, Akca A. Kars Yöresinde sığır ve köpeklerde Neospora caninum üzerine epi-demiyolojik araştırmalar: Gruplararası çalışma. Kafkas Univ Vet Fak Derg. 2012; 18(Suppl-A), A193-9.DOI: 10.9775/kvfd.2012.6181
38. Kul O, Kabakci N, Yildiz K, et al. Neospora caninum associated with epidemic abortions in dairy cattle: the first clinical neosporosis report in Turkey. Vet Parasitol.2009; 159(1):69-72.DOI: 10.1016/j.vetpar. 2008.10.019
39. Balkaya I, Bastem Z, Avcioglu H, et al. Se-roprevalence of Neospora caninum Antibod-ies in Cattle in Eastern Turkey. Isr J Vet Med.2009; 67(2):109-12.
40. Celik HA, Kozan E, Eser M, et al. A re-search on seroprevalence of Neospora cani-num in cattle. Ankara Univ Vet Fak Derg, 2013; 60:99-102.DOI: 10.1501/Vetfak_0000002561
41. Karatepe B, Karatepe M. Seroprevalence of Neospora caninum in cattle in Nigde Prov-ince, Turkey. Isr J Vet Med.2016; 71:39-42.
42. Yildiz K, Gokpinar S, Sursal N, et al. Sero-prevalence of Neospora caninum in Dairy Cattle Raised in Cicekdagi District of Kirsehir Province. Turkiye Parasitol Derg.2017; 41(3):135-8.DOI: 10.5152/tpd.2017.5218
43. Eski F, Utuk AE. Detection of Anti-Neospora caninum Antibodies in Cattle in Adana Province of Turkey. Van Vet J. 2018; 29(2):93-9.
44. Kasap S, Ertunc S, Temizel EM, et al. A study of Neospora caninum antibody sero-prevalence in dairy cows in Turkey. J Hel-lenıc Vet Med Soc, 2020; 71:2019-22.DOI: 10.12681/jhvms.22950
45. Wouda W, Moen AR, Schukken YH. Abortion risk in progeny of cows after a Neospora caninum epidemic. Theriogenolo-gy.1998; 49(7):1311-6.DOI: 10.1016/S0093-691X(98)00078-8
46. Thurmond MC, Hietala SK. Culling asso-ciated with Neospora caninum in dairy cows. Am J Vet Res.1996; 57:1559-62.
47. Sanderson MW, Gay JM, Baszler TV. Ne-ospora caninum seroprevalence and associat-ed risk factors in beef cattle in the North-western United States. Vet Parasitol.2000; 90:15-24.DOI: 10.1016/s0304-4017(00)00234-x
48. Sadrebazzaz A, Haddadzadeh H, Esmailnia K, et al. Serological prevalence of Neospora caninum in healthy and aborted dairy cattle in Mashhad, Iran. Vet Parasitol. 2004; 124: 201-4. DOI: 10.1016/j.vetpar.2004.06.027
49. Dumanli N, Aktas M. Toxoplasmatidae (Toxoplasma, Neospora). In: Dumanli N, Karaer Z, editors. Veteriner Protozooloji. Ankara; Medisan:2010. p. 128-35.
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| Issue | Vol 16 No 3 (2021) | |
| Section | Original Article(s) | |
| DOI | https://doi.org/10.18502/ijpa.v16i3.7091 | |
| Keywords | ||
| Abortion Cow İnfertility Neospora caninum Turkey | ||
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How to Cite
1.
Köse O, Adanır R, Kocamüftüoğlu M, Çetin Y. Investigation of Neospora caninum Seroprevalence and Asso-ciation with Reproductive Problems in Cows in Burdur Province of Turkey. Iran J Parasitol. 2021;16(3):386-393.
