Short Communication

The effects of Ostertagia occidentalis somatic antigens on ovine TLR2 and TLR4 expression

Abstract

Background: Recognition of helminth-derived pathogen associated molecular patterns (PAMPs) by pattern recognition receptors (PRRs), including toll like recep­tors (TLRs) is the first step towards initiating anti–helminth immune re­sponses.

Methods: Using somatic antigens of Ostertagia occidentalis, an important abomasal parasite of ruminants, the expression of ovine TLR2 and TLR4 in peripheral blood mononuclear cells (PBMCs) was analyzed by real-time quatitative reverse-transcrip­tion polymerase chain reaction (qRT-PCR). Somatic antigens of O. occidentalis were prepared to stimulate ovine PBMCs in a time and dose dependent manner.

Results: A high expression of TLR2 and TLR4 was observed in PBMCs cultured with somatic antigens of the parasites specially when PBMCs were cultured with 100 µg/ml of somatic antigens and incubated for 2h. Up-regulation of TLR2 expres­sion was more pronounced and evident in our study.

Conclsusion: Somatic antigens of O. occidentalis have immunostimulatory and domi­nant role on peripheral immune cells. This study provide for the first time evidence of induction of TLRs in ovine PBMCs by somatic antigen of O. occidentalis

Simpson HV. Pathophysiology of abomasal parasitism: is the host or parasite responsible? Vet J. 2000; 160: 177–191.

Simpson HV, Przemeck SM, Scott I, Thomas DG, Green RS, Reynolds GW. Pathophysiol-ogy in Teladorsagia circumcincta-infected sheep se-lected for high fleece weight. Vet Parasitol. 2009; 163(1-2):73-80.

Shayan P, Eslami A, Borji H. Innovative re-striction site created PCR-RFLP for detection of benzimidazole resistance in Teladorsagia cir-cumcincta. Parasitol Res. 2007; 100(5):1063-8.

Papadopoulos E, Gallidis E, Ptochos S. Anthel-mintic resistance in sheep in Europe: a selected review. Vet Parasitol. 2012; 189(1):85-8.

Beraldi D, Craig BH, Bishop SC, Hopkins J, Pemberton JM. Phenotypic analysis of host-parasite interactions in lambs infected with Teladorsagia circumcincta. Int J Parasitol. 2008;38: 1567–1577.

McNeilly TN, Devaney E, Matthews JB. Teladorsagia circumcincta in the sheep abomasum: defining the role of dendritic cells in T cell reg-ulation and protective immunity. Parasite Im-munol. 2009; 31(7):347-56.

McGuinness DH, Dehal PK, Pleass R J . Pat-tern recognition molecules and innate immun-ity to parasites. Trends Parasitol. 2003; 19 :7.

Iwasaki A, Medzhitov R. Toll-like receptor con-trol of the adaptive immune responses. Nat Immunol. 2004; 5: 987–995.

Kawai T, Akira S. The role of pattern-recogni-tion receptors in innate immunity: update on Toll-like receptors. Nat Immunol. 2010; 11: 373–384.

Pfaffl MW. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 2001; 29: 2002-2007.

Kane CM, Jung E, Pearce EJ. Schistosoma man-soni egg antigen-mediated modulation of Toll-like receptor (TLR)-induced activation occurs independently of TLR2, TLR4, and MyD88. Infect Immun. 2008; 76: 5754–5759.

Chen XM, O’Hara SP, Nelson JB, Splinter PL, Small AJ, Tietz PS, Limper AH, LaRusso NF. Multiple TLRs are expressed in human cholan-giocytes and mediate host epithelial defense re-sponses to Cryptosporidium parvum via activation of NF-kappaB. J Immunol. 2005; 175:7447–7456.

Shan JY, Ji WZ, Li HT, Tuxun T, Lin RY, Wen H. TLR2 and TLR4 expression in periph-eral blood mononuclear cells of patients with chronic cystic echinococcosis and its relation-ship with IL-10. Parasite Immun. 2011; 33: 692–696.

Kosik-Bogacka DI, Wojtkowiak-Giera A, Ko-lasa A, Czernomysy-Furowicz D, Lanocha N, Wandurska-Nowak E, Salamatin R, Jag-odzinski PP. Hymenolepis diminuta: analysis of the expression of Toll-like receptor genes (TLR2 and TLR4) in the small and large intestines of rats. Part II. Exp Parasitol. 2013; 135(2):437-45.

Van Riet E, Everts B, Retra K. Combined TLR2 and TLR4 ligation in the context of bac-terial or helminth extracts in human monocyte derived dendritic cells: molecular correlates for Th1/Th2 polarization. BMC Immunol. 2009; 10: 9–20.

Mun HS, Aosai F, Norose K, Piao LX, Fang H, Akira S, Yano A . Toll-like receptor 4 mediates tolerance in macrophages stimulated with Toxo-plasma gondii-derived heat shock protein 70. In-fect Immun. 2005; 73: 4634–4642.

Seo M, Guk SM, Han ET, Chai JY. Role of intestinal goblet cells in the expulsion of Gym-nophalloides seoi from mice. J Parasitol. 2003; 89: 1080–1082.

Cario E, Rosenberg IM, Brandwein SL, Beck PL, Reinecker HC, Podolsky DK. Lipopolysac-charide activates distinct signaling pathways in intestinal epithelial cell lines expressing TLRs. J Immunol. 2000; 164: 966–972.

Moncada DM, Kammanadiminti SJ, Chadee K. Mucin and Toll-like receptors in host defense against intestinal parasites. Trends Parasitol. 2003; 19:7.

Peterson CY, Costantini TW, Loomis WH, Putnam JG, Wolf P, Bansal V, Eliceiri BP, Baird A, Coimbra R. Toll-like receptor-4 medi-ates intestinal barrier breakdown after thermal injury. Surg Infect. 2010; 11: 137–144.

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IssueVol 10 No 3 (2015) QRcode
SectionShort Communication(s)
Keywords
Ostertagia occidentalis Peripheral blood mononuclear cells Toll-like receptors
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Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
How to Cite
1.
BORJI H, HAGHPARAST A, SOLEIMANI N, AZIZZADEH M, NAZEMSHIRAZI MH. The effects of Ostertagia occidentalis somatic antigens on ovine TLR2 and TLR4 expression. Iran J Parasitol. 2015;10(3):498-504.