Seroepidemiological Study of Toxocariasis among Volunteers Animal Husbandry Workers and Veterinary in Southern Anatolia in Turkey in 2014
-
Hamdi SOZEN
,
Burak E CITIL
,
Selmin CAYLAK
,
Aysegul A GOKMEN
,
Selçuk KAYA
,
Mustafa DEMIRCI
,
Metin KORKMAZ
,
Cem SAHIN
,
Ismail KIRLI
Abstract
Background:Human toxocariasis is a parasitic infection caused by the larvae of Toxocaracanis. We examine the Toxocara seroprevalance in veterinarians and animal husbandry workers living in the Mugla Province, Turkey to evaluate better the risk factors for Toxocara exposure.
Methods: In 2014, 376 volunteers participated in the study in 2014. All blood specimens were tested using a commercial enzyme immunoassay kit and ELISA positive samples were confirmed by Western Blot (WB) method.
Results: The seroprevalence of Toxocara, as determined by WB, was 8%. A statistically significant correlation was evident between patient age and Toxocara positivity among animal husbandry workers (P = 0.029). A strong association was also evident between sex and seropositivity in the animal husbandry group (P=0.024). Veterinarians working in pet clinics did in fact exhibit higher Toxocara seropositivities relative to those of other groups (P = 0.029). A statistically significant difference was detected between the rural geographic areas surveyed (P = 0.04).
Conclusion: In Mugla Province, seroprevalence of Toxocara is lower than other regions. Despite the low seroprevalence observed, especially in high risk professions toxocariasis remains an important medical concern within the region.
Selek MB, Baylan O. Human Toxocariasis. Turk Hij Den Biyol Derg. 2013; 70(2): 113–34.
Khazan H, Khazaei M, Seyyid Tabaee SJ, Mehrabi A. Prevalence of Toxocara spp. eggs in public parks in Tehran City, Iran. Iran J Parasitol. 2012;7(3):38-42.
Despommier D. Toxocariasis: clinical as-pects, epidemiology, medical ecology, and molecular aspects. Clin Microbiol Rev. 2003; 16(2): 265-72.
Won KY, Kruszon-Moran D, Schantz PM, Jones JL. National seroprevalence and risk factors for Zoonotic Toxocara spp. infec-tion. Am J Trop Med Hyg. 2008;79(4):552-7.
Aydenizöz-Ozkayhan M, Yağci BB, Erat S. The investigation of Toxocara canis eggs in coats of different dog breeds as a potential transmission route in human toxocariasis. Vet Parasitol. 2008;152(1-2): 94-100.
Pawlowski Z. Toxocariasis in humans: clinical expression and treatment dilemma. J Helminthol. 2001; 75(4): 299-305.
Yamasaki H, Araki K, Lim PK, Zasmy N, Mak JW, Taib R, Aoki T. Development of a highly specific recombinant Toxocara canis second-stage larva excretory-secretory anti-gen for immunodiagnosis of human toxo-cariasis. J Clin Microbiol. 2000;38(4): 1409-13.
Magnaval JF, Fabre R, Maurieres P, Char-let JP, de Larrard B. Application of the western blotting procedure for the immu-nodiagnosis of human toxocariasis. Par-asitol Res. 1991; 77(8): 697-702.
Lee AC, Schantz PM, Kazacos KR, Mont-gomery SP, Bowman DD. Epidemiologic and zoonotic aspects of ascarid infections in dogs and cats. Trends Parasitol. 2010 Apr;26(4):155-161.
Korkmaz M. Visceral larva migrans. İkinci evre Toxocara canis larvalarının in vitro kültürü, ekskretuvar/sekretuvar antijenin elde edilmesi ve ELISA yöntemi ile tanısı (Uzmanlık tezi). Ege Üniversitesi Tıp Fakültesi, Parazitoloji Anabilim Dalı, İzmir. (1998)
Laemmli UK. Cleavage of structural pro-teins during the assembly of the head of bacteriophage T4. Nature. 1970;227:680–5.
Yazar S, Yaman O, Cetinkaya U, Hamamci B, Sahin I. Investigation of anti-Toxocara ca-nis IgG antibodies in patients presenting at the Erciyes University Medical Faculty, Department of Parasitology. Turkiye Parazitol Derg. 2010; 34(1):24-6.
Demirci M, Kaya S, Cetin E, Arıdoğan B, Onal S, Korkmaz M. Seroepidemiological investigation of toxocariasis in the isparta region of Turkey. Iran J Parasitol. 2010;5(2):52-9.
Büyükbaba O, Ozkan E, Buget E. Toxocara canis ve çocuklardaki seroprevalansının ELISA ile araştırılması. Turkish J Infect. 1996;10(1):7-11.
Cong W, Zhang XX, Zhou N, Yu CZ, Chen J, Wang XY, Li B, Qian AD, Zhu XQ. Toxocara seroprevalence among clini-cally healthy individuals, pregnant women and psychiatric patients and associated risk factors in Shandong Province, Eastern China. PLoS Negl Trop Dis. 2014;8(8):e3082.
Sadjjadi SM, Khosravi M, Mehrabani D, & Oryan A. Seroprevalence of Toxocara infec-tion in school children in Shiraz, Southern Iran. J Trop Pediatr. 2000; 46: 327-30.
Fallah M, Azimi A, Taherkhani H. Sero-prevalence of toxocariasis in children aged 1-9 years in western Islamic Republic of Iran, 2003. Eastern Mediterr Health J. 2007;13(5), 1073-7.
Zwolinski J. The risk factors of Toxocara canis infestation in population of patients from the Lublin region. Wiad Parazytol. 2000; 46(4): 63-73.
Kaplan M, Kalkan A, Kuk S, Demirdag K, Ozden M, Kilic SS. Toxocara seropreva-lence in schizophrenic patients in Turkey. Yonsei Med J. 2008; 49(2):224-9.
Chiodo P, Basualdo J, Ciarmela L, Pezzani B, Apezteguia M, Minvielle M. Related fac-tors to human toxocariasis in a rural com-munity of Argentina. Mem Inst Oswaldo Cruz. 2006;101(4):397-400.
Romano N, Nor Azah MO, Rahmah N, Yal L, Rohela M. Seroprevalence of toxo-cariasis among orang asli (indigenous peo-ple) in Malaysia using two immunoassays. Trop Biomed. 2010; 27(3):585-94.
Stensvold CR, Skov J, Moller LN, Jensen PM, Kapel CM, Petersen E, et al. Sero-prevalence of human toxocariasis in Den-mark. Clin Vaccine Immunol. 2009; 16(9): 1372-3.
Fan CK, Hung CC, Du WY, Liao CW, Su KE. Seroepidemiology of Toxocara canis in-fection among mountain aboriginal school-children living in contaminated districts in eastern Taiwan. Trop Med Int Health, 2004; 9(12): 1312-8.
Santarem VA, Leli FN, Rubinsky-Elefant G, Giuffrida R. Protective and risk factors for toxocariasis in children from two dif-ferent social classes of Brazil. Rev Inst Med Trop Sao Paulo. 2011; 53(2): 66-72.
Doğan N, Dinleyici EC, Bor O, Töz SO, Ozbel Y. Seroepidemiological survey for Toxocara canis infection in the northwestern part of Turkey. Türkiye Parazitol Derg. 2007; 31(4): 288-91.
Smith H, Holland C, Taylor M, Magnaval JF, Schantz P, Maizels R. How common is human toxocariasis? Towards standardiz-ing our knowledge. Trends Parasitol. 2009; 25(4): 182-8.
Castillo D, Paredes C, Zanartu C, Castillo G, Mercado R, Munoz V, et al. Environ-mental contamination with Toxocara sp. eggs in public squares and parks from San-tiago, Chile, 1999. Bol Chil Parasitol. 2000; 55(3-4): 86-91.
Giacometti A, Cirioni O, Fortuna M, Osi-mani P, Antonicelli L, Del Prete MS, et al.
Environmental and serological evidence for the presence of toxocariasis in the ur-ban area of Ancona, Italy. Eur J Epi-demiol. 2000; 16(11): 1023-6.
Rubinsky-Elefant G, da Silva-Nunes M, Malafronte RS, Muniz PT, Ferreira MU. Human toxocariasis in rural Brazilian ama-zonia: seroprevalence, risk factors, and spa-tial distribution Am J Trop Med Hyg. 2008; 79(1): 93-8.
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| Issue | Vol 10 No 3 (2015) | |
| Section | Original Article(s) | |
| Keywords | ||
| Toxocara Seroprevalence Veterinary Animal husbandry workers Turkey | ||
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