Recombinant LPG3 Stimulates IFN-Γ and TNF-Α Secretion by Human NK Cells
-
Sanaz RASOLZADEH
,
Mostafa HAJI FATAHALIHA
,
Maryam HOSSEINI
,
Reza JAFARI
,
Abolfazl MIAHIPOUR
,
Sevil SADREDDINI
,
Zohreh BABALO
,
Hossein SAMADI KAFIL
,
Mehdi YOUSEFI
Abstract
Background:Natural killer (NK) cells play an important role in early stages of innate immune responses against viral and tumoral attacks. Activation of NK cells by leishmaniasis results in secretion of cytokines such as interferon (IFN)-γ and tumor necrosis factor (TNF)-α, which enhance the phagocytosis and clearance of parasite. Lipophosphoglycan 3 (LPG3), the Leishmania homologous with GRP94 (glucose regulated protein 94), a member of HSP90 family, contributes to LPG assembly as the most abundant macromolecule on the surface of Leishmania promastigotes.
Methods:We purified NK cells from healthy individuals (n=10) using magnetic-activated cell sorting (MACS) technology. Purified NK cells were co-incubated with different concentrations of recombinant LPG3 (rLPG3), and its N-terminal (NT) and C-terminal (CT) fragments. Finally, the production of IFN-γ and TNF-α by NK cells were measured by ELISA.
Results:Recombinant LPG3 but not its fragments (CT and NT), could significantly enhance the production of TNF-α by NK cells (P<0.05). Moreover, rLPG3, CT, and NT fragments were markedly stimulated the secretion of IFN-γ by NK cells (P<0.001).
Conclusion: The Leishmania LPG3 antigen could effectively activate NK cells, in vitro. Leishmania LPG3 participates in the innate immunity against leishmaniasis and thereby improves the effective parasite destruction.However, its efficiency should be tested in vivo.Alvar J, Velez ID, Bern C, Herrero M, Desjeux P, Cano J, Jannin J, den Boer M, Team WLC. Leishmaniasis worldwide and global estimates of its incidence. PloS ONE. 2012;7:e35671.
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| Issue | Vol 10 No 3 (2015) | |
| Section | Original Article(s) | |
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| Leishmania LPG3 Natural killer cell TLR | ||
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