Following Up of Surgical Treated Human Liver Cystic Echino-coccosis: A Proteomics Approach
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Abstract
Background: Cystic echinococcosis (CE) is one of the most important parasitic zoonosis in the world. Post-surgery follow up in CE patients is an important non-solved problem up to now. Therefore, the investigations on this problematic issue would be very applicable in the view of CE clinical treatment.
Methods: A total of 24 confirmed liver CE patients sera including eight sera before surgery (BS), eight sera three months post-surgery (3MPS), and eight sera six months post-surgery (6MPS) were used in the present study. Proteomics methods including 2DE and LC-MS/MS were performed on the specimens followed by bioinformatics analysis such as Gene Ontology (GO) and Protein-Protein Interaction (PPI) network analysis.
Results: A total of 235 proteins were detected of which 12 differentially expressed proteins (DEP) were identified by LC-MS/MS in all sera. The proteins were presented in BS and suppressed after surgery as follows: HPX, SERPINA1, SERPINC1, CP, HBD, and HBA2. Comparisons of the protein expression in sera of patients BS, 3MPS, and 6MPS revealed that GC, IGJ, AHSG, CD5L, FGG, and APOC3 have been overexpressed in 3MPS and 6MPS. PPI network analysis demonstrated that SERPINC1 and AHSG with more connection in the network could be considered as hub proteins and potential prognostic biomarkers in response to surgical treatment of liver CE.
Conclusion: Application of proteomics methods on patient’s sera could be used as a novel biomarker tool for following-up liver CE patients. In this regards, proteomics and, application of bioinformatics analysis including GO and PPI showed that SERPINC1, AHSG and HPX are of more value as a potential follow up biomarkers in response to surgical treatment.
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24. Sadjjadi FS, Rezaie-Tavirani M, Ahmadi NA, et al. Proteome evaluation of human cystic echinococ-cosis sera using two dimensional gel electrophore-sis. Gastroenterol Hepatol Bed Bench. 2018; 11(1):75–82.
25. Garcia-Obregon S, Azkargorta M, Seijas I, et al. Identification of a panel of serum protein markers in early stage of sepsis and its validation in a cohort of patients. J Microbiol Immunol Infect. 2018; 51(4):465–72.
26. Da’dara AA, Siddons G, Icaza M, et al. How schis-tosomes alter the human serum proteome. Mol Bi-ochem Parasitol. 2017; 215:40–6.
27. Haddad JG. Plasma vitamin D-binding protein (Gc-globulin): Multiple tasks. J Steroid Biochem Mol Biol. 1995; 53(1–6):579–82.
28. Perga S, Albo AG, Lis K, et al. Vitamin D binding protein isoforms and apolipoprotein E in cerebro-spinal fluid as prognostic biomarkers of multiple sclerosis. PLoS One. 2015; 10(6):e0129291.
29. Zhao ZW, Lin CG, Wu LZ, et al. Serum fetuin-A levels are associated with the presence and severity of coronary artery disease in patients with type 2 di-abetes. Biomarkers. 2013; 18(2):160–4.
30. Delanghe JR, Langlois MR. Hemopexin: a review of biological aspects and the role in laboratory med-icine. Clin Chim Acta. 2001; 312(1–2):13–23.
31. Aziz A, Zhang W, Li J, et al. Proteomic characteri-zation of Echinococcus granulosus hydatid cyst fluid from sheep, cattle and humans. J Proteomics. 2011; 74(9):1560–72.
32. Hunt JM, Tuder R. Alpha 1 anti-trypsin: one pro-tein, many functions. Curr Mol Med. 2012; 12(7):827–35.
33. He T, Hu JY, Han J, et al. Identification of differ-entially expressed serum proteins in infectious pur-pura fulminans. Dis Markers. 2014; 2014:698383.
34. Zeghir-Bouteldja R, Polomé A, Bousbata S, et al. Comparative proteome profiling of hydatid fluid from Algerian patients reveals cyst location-related variation in Echinococcus granulosus. Acta Trop. 2017; 171:199–206.
35. Ahn C-S, Kim J-G, Han X, et al. Comparison of Echinococcus multilocularis and Echinococcus granulosus hy-datid fluid proteome provides molecular strategies for specialized host-parasite interactions. Oncotar-get. 2017; 8(57):97009–24.
2. Ahmadi N, Dalimi A. Characterization of Echinococ-cus granulosus isolates from human, sheep and camel in Iran. Infect Genet Evol. 2006; 6(2):85–90.
3. Ahmadi NA, Hamidi M. Unusual localizations of human hydatid disease in Hamedan province, west of Iran. Helminthologia. 2010;47(2): 94-98.
4. Manzano-Román R, Sánchez-Ovejero C, Hernán-dez-González A, et al. Serological Diagnosis and Follow-Up of Human Cystic Echinococcosis: A New Hope for the Future? Biomed Res Int. 2015; 2015:428205.
5. Mirzapour A, Seyyed Tabaei S, Bandehpour M, et al. Designing a Recombinant Multi-Epitope Anti-gen of Echinococcus granulosus to Diagnose Human Cystic Echinococcosis. Iran J Parasitol. 2020; 15(1):1–10.
6. Noordin R, Khanbabaie S, Yunus MH, et al. Eval-uation of the Diagnostic Performance of Recombi-nant Antigen B1 for Detection of Cystic Echino-coccosis Using Lateral Flow Dipstick Test. Iran J Parasitol. 2020; 15(3):290–8.
7. Stojković M, Weber TF, Junghanss T. Clinical man-agement of cystic echinococcosis: State of the art and perspectives. Curr Opin Infect Dis. 2018; 31(5):383–92.
8. Brunetti E, Kern P, Vuitton DA, et al. Expert con-sensus for the diagnosis and treatment of cystic and alveolar echinococcosis in humans. Acta Trop. 2010; 114(1):1–16.
9. Neumayr A, Tamarozzi F, Goblirsch S, et al. Spinal Cystic Echinococcosis – A Systematic Analysis and Review of the Literature: Part 2. Treatment, Follow-up and Outcome. PLoS Negl Trop Dis. 2013; 7(9):e2458.
10. Pagnozzi D, Addis MF, Biosa G, et al. Diagnostic accuracy of antigen 5-based ELISAs for human cystic echinococcosis. PLoS Negl Trop Dis. 2016; 10(3):e0004585.
11. Zhang W, Wen H, Li J, et al. Immunology and immunodiagnosis of cystic echinococcosis: an up-date. Clin Dev Immunol. 2012; 2012:101895.
12. Khalilpour A, Kilic T, Khalilpour S, et al. Proteo-mic-based biomarker discovery for development of next generation diagnostics. Appl Microbiol Bio-technol. 2017; 101(2):475–91.
13. Ahn C-S, Han X, Bae Y-A, et al. Alteration of im-munoproteome profile of Echinococcus granulosus hy-datid fluid with progression of cystic echinococ-cosis. Parasit Vectors. 2015; 8:10.
14. Manterola C, García N, Rojas C. Aspectos gener-ales del perfil proteómico del Echinococcus granulosus. Int J Morphol. 2019; 37(2):773–9.
15. Neuhoff V, Arold N, Taube D, et al. Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G‐250 and R‐250. Electrophoresis. 1988; 9(6):255–62.
16. Hajjaran H, Mousavi P, Burchmore R, et al. Com-parative Proteomic Profiling of Leishmania tropica: In-vestigation of a Case Infected with Simultaneous Cutaneous and Viscerotropic Leishmaniasis by 2-Dimentional Electrophoresis and Mass Spectrome-try. Iran J Parasitol. 2015; 10(3):366–80.
17. Szklarczyk D, Gable AL, Lyon D, et al. STRING v11: protein-protein association networks with in-creased coverage, supporting functional discovery in genome-wide experimental datasets. Nucleic Ac-ids Res. 2019; 47(D1):D607–D13.
18. Killcoyne S, Carter GW, Smith J, et al. Cytoscape: a community-based framework for network model-ing. Methods Mol Biol. 2009; 563:219–39.
19. Rafiei A, Jahanshahi A, Talaeizadeh A. Evaluation of specific IgG antibody detection in diagnosis and post-surgical monitoring of cystic echinococcosis. Iran J Parasitol. 2008; 3(2):10–4.
20. Meng CR, Zhang Q, Zhang ZX, et al. Serum pro-teomic marker of hepatic echinococcosis screened by surface enhanced laser desorption/ionization time of flight mass spectrometry. Chinese J En-dem. 2010; 29(4):461–5.
21. Stojkovic M, Adt HM, Rosenberger K, et al. Fol-low-up of surgically treated patients with cystic echinococcosis: can novel recombinant antigens compete with imaging? Analysis of a patient cohort. Trop Med Int Health. 2017; 22(5):614–21.
22. Nouir N Ben, Gianinazzi C, Gorcii M, et al. Isola-tion and molecular characterization of recombinant Echinococcus granulosus P29 protein (recP29) and its as-sessment for the post-surgical serological follow-up of human cystic echinococcosis in young patients. Trans R Soc Trop Med Hyg. 2009; 103(4):355–64.
23. Wen H, Vuitton L, Tuxun T, et al. Echinococcosis: Advances in the 21st Century. Clin Microbiol Rev. 2019; 32(2): e00075-18.
24. Sadjjadi FS, Rezaie-Tavirani M, Ahmadi NA, et al. Proteome evaluation of human cystic echinococ-cosis sera using two dimensional gel electrophore-sis. Gastroenterol Hepatol Bed Bench. 2018; 11(1):75–82.
25. Garcia-Obregon S, Azkargorta M, Seijas I, et al. Identification of a panel of serum protein markers in early stage of sepsis and its validation in a cohort of patients. J Microbiol Immunol Infect. 2018; 51(4):465–72.
26. Da’dara AA, Siddons G, Icaza M, et al. How schis-tosomes alter the human serum proteome. Mol Bi-ochem Parasitol. 2017; 215:40–6.
27. Haddad JG. Plasma vitamin D-binding protein (Gc-globulin): Multiple tasks. J Steroid Biochem Mol Biol. 1995; 53(1–6):579–82.
28. Perga S, Albo AG, Lis K, et al. Vitamin D binding protein isoforms and apolipoprotein E in cerebro-spinal fluid as prognostic biomarkers of multiple sclerosis. PLoS One. 2015; 10(6):e0129291.
29. Zhao ZW, Lin CG, Wu LZ, et al. Serum fetuin-A levels are associated with the presence and severity of coronary artery disease in patients with type 2 di-abetes. Biomarkers. 2013; 18(2):160–4.
30. Delanghe JR, Langlois MR. Hemopexin: a review of biological aspects and the role in laboratory med-icine. Clin Chim Acta. 2001; 312(1–2):13–23.
31. Aziz A, Zhang W, Li J, et al. Proteomic characteri-zation of Echinococcus granulosus hydatid cyst fluid from sheep, cattle and humans. J Proteomics. 2011; 74(9):1560–72.
32. Hunt JM, Tuder R. Alpha 1 anti-trypsin: one pro-tein, many functions. Curr Mol Med. 2012; 12(7):827–35.
33. He T, Hu JY, Han J, et al. Identification of differ-entially expressed serum proteins in infectious pur-pura fulminans. Dis Markers. 2014; 2014:698383.
34. Zeghir-Bouteldja R, Polomé A, Bousbata S, et al. Comparative proteome profiling of hydatid fluid from Algerian patients reveals cyst location-related variation in Echinococcus granulosus. Acta Trop. 2017; 171:199–206.
35. Ahn C-S, Kim J-G, Han X, et al. Comparison of Echinococcus multilocularis and Echinococcus granulosus hy-datid fluid proteome provides molecular strategies for specialized host-parasite interactions. Oncotar-get. 2017; 8(57):97009–24.
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| Issue | Vol 16 No 1 (2021) | |
| Section | Original Article(s) | |
| DOI | https://doi.org/10.18502/ijpa.v16i1.5507 | |
| Keywords | ||
| Proteomics Cystic echinococcosis Hydatidosis | ||
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How to Cite
1.
SADJJADI FS, AHMADI NA, REZAIE-TAVIRANI M, ZALI H. Following Up of Surgical Treated Human Liver Cystic Echino-coccosis: A Proteomics Approach. Iran J Parasitol. 2021;16(1):11-12.
