Isolation and Molecular Identification of Acanthamoeba and Naegleria from Agricultural Water Canal in Qazvin, Iran

  • Mandana KHORSANDI RAFSANJANI Student Research Committee, Qazvin University of Medical Sciences, Qazvin, Iran
  • Elham HAJIALILO Mail Cellular and Molecular Research Center, Research Institute for Prevention of Non-Communicable Diseases, Qazvin University of Medical Sciences, Qazvin, Iran AND Department of Parasitology and Mycology, Qazvin University of Medical Sciences, Qazvin, Iran
  • Mehrzad SARAEI Cellular and Molecular Research Center, Research Institute for Prevention of Non-Communicable Diseases, Qazvin University of Medical Sciences, Qazvin, Iran AND Department of Parasitology and Mycology, Qazvin University of Medical Sciences, Qazvin, Iran
  • Safar Ali ALIZADEH Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran AND Department of Microbiology, Qazvin University of Medical Sciences, Qazvin, Iran
  • Amir JAVADI Department of Social Sciences, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran
Acanthamoeba, Naegleria, Genotype, Agricultural water, Iran


Background: Free-living amoeba (FLA) are widely distributed in different environmental sources. The most genera of the amoeba are Acanthamoeba, Naegleria and Vermamoeba. The most common consequences of the infections in immune-deficient and immunocompetent persons are amoebic encephalitis and keratitis. The aim of this study was to investigate the presence of Acanthamoeba spp. and Naegleria spp., isolated from the main agricultural water canal in Qazvin.

Methods: Totally, 120 water specimens were collected and later the specimens were cultured and cloned to identify positive samples. PCR amplification and sequencing were carried out to identify the isolated species as well as the genotypes of amoeba.

Results: According to morphological surveys, 41.7% (50/120) of water specimens were positive for FLA. Molecular analysis revealed that 68.6% and 31.4% of Acanthamoeba specimens were identified as T3 and T4 genotypes, respectively. Also, two species of Naegleria named as N. lovaniensis (57.1%) and Naegleria spp. (42.8%) were identified. The results of pathogenicity assays demonstrated that 38.5% of T3 and 61.5% of T4 genotypes of Acanthamoeba were highly pathogenic parasites.

Conclusion: The water flowing in the agricultural canal of the area is contaminated with potential pathogenic FLA, therefore, it is recommended that more attention to be paid towards proper treatment of water sources to prevent possible risk of the disease.


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How to Cite
KHORSANDI RAFSANJANI M, HAJIALILO E, SARAEI M, ALIZADEH SA, JAVADI A. Isolation and Molecular Identification of Acanthamoeba and Naegleria from Agricultural Water Canal in Qazvin, Iran. Iran J Parasitol. 15(3):393-402.
Original Article(s)