Evaluation of Toll-Like Receptor 11 Agonist Adjuvant Activity in Immunization of BALB/c Mice with Total Lysate Antigens of Toxoplasma gondii RH Strain

  • Meysam SHOKRI Department of Parasitology and Mycology, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran
  • Khosro HAZRATI TAPPEH Department of Parasitology and Mycology, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran
  • Elyar MESHKINI Department of Parasitology and Mycology, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran
  • Arash AMINPOUR Mail Department of Parasitology and Mycology, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran
Keywords:
Toxoplasma gondii, Adjuvant, Toll-like receptor 11, Immunization, Toxoplasma lysate antigen

Abstract

Background: In this study, the effect of total lysate antigen (TLA) of Toxoplasma gondii on spleen lymphocyte prolifration, secretion of IL5, INF-γ, and mice survival time was evaluated using agonist of toll-like receptor (TLR) 11, as an adjuvant.

Results: Mice immunized with TLA + adjuvant showed higher immunization index than the two other groups and combination of TLR11 (as an adjuvant) and TLA significantly elevated the effect of TLA by increasing the production of INF-γ and IL-5 and by the shift of the immune system to Th1. In addition, the combination of TLA and TLR11 adjuvant increased the proliferation of lymphocytes and survival time in mice against T. gondii.

Conclusion: Profilin (as an adjuvant) in combination with TLA could be a potent vaccine candidate that evokes a powerful specific immune response and significantly improves the efficacy of TLA vaccine by increasing the induction of INF-γ production and by shifting the immune responses to Th1 profile through increasing the INF-γ/IL-5 ratio. It causes significant protection against T. gondii after i.p. injection.

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Published
2020-09-12
How to Cite
1.
SHOKRI M, HAZRATI TAPPEH K, MESHKINI E, AMINPOUR A. Evaluation of Toll-Like Receptor 11 Agonist Adjuvant Activity in Immunization of BALB/c Mice with Total Lysate Antigens of Toxoplasma gondii RH Strain. Iran J Parasitol. 15(3):349-356.
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Original Article(s)