Loop-Mediated Isothermal Amplification (LAMP) Assay to De-tect Toxoplasmosis in Schizophrenia Patients

  • Hadi MIRAHMADI Infectious Diseases and Tropical Medicine Research Center, Resistant Tubercluosis Institute, Zahedan University of Medical Sciences, Zahedan, Iran AND Department of Parasitology and Mycology, Faculty of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran
  • Raheleh HASANZADEH Infectious Diseases and Tropical Medicine Research Center, Resistant Tubercluosis Institute, Zahedan University of Medical Sciences, Zahedan, Iran AND Department of Parasitology and Mycology, Faculty of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran
  • Hamid MALEK RAEESI Infectious Diseases and Tropical Medicine Research Center, Resistant Tubercluosis Institute, Zahedan University of Medical Sciences, Zahedan, Iran
  • Shirzad FALLAHI Department of Parasitology and Mycology, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran
  • Mahdi KHOSHSIMA SHAHRAKI Department of Medical Parasitology, Zabol University of Medical Sciences, Zabol, Iran
  • Alireza BADIRZADEH Mail Department of Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
Keywords:
Loop-mediated isother-mal amplification (LAMP), Toxoplasma gondii, Toxoplasmosis, Schizophrenia

Abstract

Background: Toxoplasma gondii (T. gondii) causes an important parasitic infection known as toxoplasmosis, which is a globally distributed important zoonosis. One of the major serious characteristics of T. gondii is its ability to manipulate the behavior of intermediate hosts. We performed a cross-sectional study to determine toxoplasmosis in schizophrenic patients, as one of the major neuropsychiatric disorders, using loop-mediated isothermal amplification (LAMP) technic by targeting parasite B1 gene.

Methods: Blood samples were taken from 118 schizophrenic patients hospitalized in tow hospitals including Baharan, Clinic of Psychiatric Ali-ibn-Abi-Talib Hospital (in Zahedan City), and Amir-al Momenin Psychiatric Hospital (in Zabol City), Sistan and Baluchestan Province, southeast Iran in 2016. They were analyzed using LAMP, and compared with the previous data of nested-PCR and serology.

Results: Out of the 118 schizophrenic individuals, 56 patients (47.4%) were found to be infected with T. gondii. The diagnosis of toxoplasmosis was confirmed in 41 patients (34.7%) via the nested-PCR. The seroprevalence of toxoplasmosis in schizophrenic patients was 55.9% (66/118).

Conclusion: We found a high efficiency of LAMP method in identifying toxoplasmosis and its high prevalence among schizophrenic patients. Our findings could provide viable offer implications for the prevention of schizophrenia.

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Published
2020-09-12
How to Cite
1.
MIRAHMADI H, HASANZADEH R, MALEK RAEESI H, FALLAHI S, KHOSHSIMA SHAHRAKI M, BADIRZADEH A. Loop-Mediated Isothermal Amplification (LAMP) Assay to De-tect Toxoplasmosis in Schizophrenia Patients. Iran J Parasitol. 15(3):299-306.
Section
Original Article(s)