In-Vitro Effect of Kalanchoe daigremontiana and Its Main Component, Quercetin against Entamoeba histolytica and Trichomonas vaginalis
Background: Parasitic infections represent one of the main public health problems in humans according to the WHO. Therefore, the need has arisen to find new treatments that can be used as an alternative cure to parasitosis. We aimed to investigate the in-vitro effects of the methanolic extract of Kalanchoe daigremontiana as well as its main component, quercetin against Entamoeba histolytica and Trichomonas vaginalis.
Methods: For this purpose, the in-vitro activity of the methanol extract of K. daigremontiana also its main component, quercetin, against trophozoites of E. histolytica and T. vaginalis was evaluated, using the microassay technique. Furthermore, the antioxidant activity was determined. Finally, the cytotoxic and cytoprotective capacity was determined using the hemolysis technique.
Results: The IC50 indicated that quercetin significantly (P < 0.05) inhibited the growth rate of the trophozoite stage of E. histolytica and T. vaginalis in comparison to the methanolic extract of K. daigremontiana (KalL). Also, quercetin significantly (P < 0.05) was a better antioxidant as compared with the positive control. In the evaluation of cytotoxicity effects, it could be observed that KalL as compared with quercetin exhibited more cytotoxicity against human erythrocytes. Quercetin significantly (P < 0.001) exhibited better cytoprotective activity compared to KalL.
Conclusion: Both K. daigremontiana methanolic extract and quercetin alone demonstrated high antiparasitic activity against E. histolytica and T. vaginalis. However, the in-vivo efficacy of K. daigremontiana and quercetin also requires to be evaluated using an animal model.
2. Theel ES, Pritt BS. Parasites. Microbiol Spectr. 2016;4(4): 10.1128/microbiolspec.DMIH2-0013-2015.
3. Ramos F, Morán P, González E, et al. En-tamoeba histolytica and Entamoeba dispar: Pre-valence infection in a rural mexican com-munity. Exp Parasitol. 2005;110(3):327–30.
4. Mundodi V, Kucknoor AS, Chang TH, Alderete JF. A novel surface protein of Trichomonas vaginalis is regulated inde-pendently by low iron and contact with vaginal epithelial cells. BMC Microbiol. 2006;6:6.
5. Pal D, Banerjee S, Cui J, Schwartz A, Ghosh SK, Samuelson J. Giardia, Entamoe-ba, and Trichomonas enzymes activate met-ronidazole (nitroreductases) and inactivate metronidazole (nitroimidazole reductases). Antimicrob Agents Chemother. 2009;53(2):458–64.
6. Humberto CH-M, Ricardo G-F, Patricia T-G, et al. Antitumor activity of Pachycereus marginatus (DC.) Britton Rose extracts against murine lymphoma L5178Y-R and skin melanoma B16F10 cells. J Med Plants Res. 2016;10(36):635–9.
7. Gomez-Flores R, Verastegui-Rodriguez L, Quintanilla-Licea R, et al. Antitumor pro-perties of Gymnosperma Glutinosum leaf ex-tracts. Cancer Invest. 2009;27(2):149–55.
8. Fürer K, Simões-Wüst AP, Von Mandach U, Hamburger M, Potterat O. Bryophyllum pinnatum and Related Species Used in Anthroposophic Medicine: Constituents, Pharmacological Activities, and Clinical Efficacy. Planta Med. 2016;82(11–12):930–41.
9. Kolodziejczyk-Czepas J, Stochmal A. Bufadienolides of Kalanchoe species: an overview of chemical structure, biological activity and prospects for pharmacological use. Phytochem Rev. 2017;16(6):1155–71.
10. Rad MS, Epifano F, Fiorito S, Suarez JMÁ. Phytochemical Analysis and Biological In-vestigation of Nepeta juncea Benth. Differ-ent Extracts. Plants (Basel). 2020;9(5)646.
11. Muzitano MF, Tinoco LW, Guette C, Kai-ser CR, Rossi-Bergmann B, Costa SS. The antileishmanial activity assessment of unu-sual flavonoids from Kalanchoe pinnata. Phy-tochemistry. 2006;67(18):2071–7.
12. Milad R. Genus Kalanchoe (Crassulaceae): A Review of Its Ethnomedicinal, Botanical, Chemical and Pharmacological Properties. European J Med Plants. 2014;4(1):86–104.
13. Elizondo-Luévano JH, Castro-Ríos R, Sánchez-García E, et al. In Vitro Study of Antiamoebic Activity of Methanol Extracts of Argemone mexicana on Trophozoites of Entamoeba histolytica HM1-IMSS. Can J In-fect Dis Med Microbiol. 2018;2018:7453787.
14. Elizondo-Luévano JH, Hernández-García ME, Pérez-Narváez OA, Castro-Ríos R, Chávez-Montes A. Berberine, curcumin and quercetin as potential antiparasitic agents. Rev Biol Trop. 2020;68(4):1241–9.
15. Elizondo-Luevano JH, Castro-Ríos R, López-Abán J, et al. Berberine: A nemato-cidal alkaloid from Argemone mexicana against Strongyloides venezuelensis. Exp Parasi-tol. 2021;220(November 2020).
16. Elizondo-Luévano JH, Castro-Ríos R, Vi-cente B, et al. In vitro antischistosomal ac-tivity of the Argemone mexicana methanolic extract and its main component berberine. Iran J Parasitol. 2021;16(1):91–100.
17. Rodríguez-Luis O, Verde-Star J, González-Horta A, et al. Preparation of polymer na-noparticles loaded with syzygium aromati-cum essential oil: An oral potential applica-tion. Bol Latinoam y del Caribe Plantas Med y Aromat. 2020;19(1):65–76.
18. White CM, Pasupuleti V, Roman YM, Li Y, Hernandez A V. Oral turmer-ic/curcumin effects on inflammatory markers in chronic inflammatory diseases: A systematic review and meta-analysis of randomized controlled trials. Pharmacol Res. 2019;146:104280 .
19. Martínez-Delgado AJ, Castillo-Velázquez U, Báez-González JG, et al. Evaluation of the Essential Oil of Citrus paradisi as an Al-ternative Treatment against Candida albicans. Open J Stomatol. 2020;10(09):258–70.
20. Costa SS, Muzitano MF, Camargo LMM, Coutinho MAS. Therapeutic potential of Kalanchoe species: Flavonoids and other secondary metabolites. Nat Prod Com-mun. 2008;3(12):2151–64.
21. El Abdellaoui S, Destandau E, Toribio A, et al. Bioactive molecules in Kalanchoe pinna-ta leaves: Extraction, purification, and identification. Anal Bioanal Chem. 2010;398(3):1329–38.
22. Muzitano MF, Cruz EA, De Almeida AP, et al. Quercitrin: An antileishmanial flavo-noid glycoside from Kalanchoe pinnata. Plan-ta Med. 2006;72(1):81–3.
23. Kumar SSD, Houreld NN, Abrahamse H. Therapeutic potential and recent advances of curcumin in the treatment of aging-associated diseases. Molecules. 2018;23(4):835.
24. Goodhead LK, MacMillan FM. Measuring osmosis and hemolysis of red blood cells. Adv Physiol Educ. 2017;41(2):298–305.
25. Elizondo-Luevano JH, Verde-Star J, Gon-zález-Horta A, Castro-Ríos R, Hernández-García ME, Chávez-Montes A. In Vitro Effect of Methanolic Extract of Argemone mexicana against Trichomonas vaginalis. Kore-an J Parasitol. 2020;58(2):135–45.
26. Derman S, Uzunoglu D, Acar T, Arasoglu T, et al. Antioxidant activity and hemo-compatibility study of quercetin loaded plga nanoparticles. Iran J Pharm Res. 2020;19(1):424–35.
27. Dayar E, Cebova M, Lietava J, Panghyova E, Pechanova O. Antioxidant Effect of Lonicera caerulea L. in the Cardiovascular System of Obese Zucker Rats. Vol. 10, Antioxidants. 2021;10(8):1199.
28. Ávila-Blanco ME, Rodríguez MG, Moreno Duque JL, Muñoz-Ortega M, Ventura-Juárez J. Amoebicidal Activity of Essential Oil of Dysphania ambrosioides (L.) Mosyakin & Clemants in an Amoebic Liver Abscess Hamster Model. Evid Based Complement Altern Med. 2014;2014: 930208 .
|Issue||Vol 16 No 3 (2021)|
|Entamoeba histolytica; In-vitro; Kalanchoe daigremontiana; Quercetin; Trichomonas vaginalis|
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